Skip to main content
SpringerLink
Log in

Regulated production of recombinant echistatin by yeast

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

The α-mating factor pre-pro-leader sequence under the regulation of theGAL10 promoter was used to direct the secretion of echistatin by recombinant yeasts. Optimization of the culture medium and host strain increased the productivity of shake flask cultures twenty-fold to 8 mg/L. In fermentors, the production of echistatin was greater than 40 mg/L.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bayne, M. L., Applebaum, J., Chicchi, G. G., Hayes, N. S., Green, B. G. and Cascieri, M. A. (1988). Gene 66: 235–244.

    Google Scholar 

  2. Carty, C. E., Kovach, F. X., McAleer, W. J. and Maigetter, R. Z. (1987). J. Indus. Microbiol. 2: 117–121.

    Google Scholar 

  3. Das, R. G. and Schultz, J. L. (1987). Biotechnol. Prog. 3: 43–48.

    Google Scholar 

  4. Can, Z.-R., Condra, J. H., Gould, R. J., Zivin, R. A., Bennett, C. D., Jacobs, J. W., Friedman, P. A. and Polokoff, M. A. (1989). Gene 79: 159–166.

    Google Scholar 

  5. Gan, Z.-R., Gould, R. J., Jacobs, J. W., Friedman, P. A. and M. A. Polokoff. (1988). J. Biol. Chem. 263: 19827–19832.

    Google Scholar 

  6. Garsky, V. M., Lumma, P. K., Freidinger, R. M., Pitzenburger, S. M., Randall, W. C., Veber, D. F., Gould, R. J., and Friedman, P. A. (1989). Proc. Natl. Acad. Sci. USA 86: 4022–4026.

    Google Scholar 

  7. Jacobson, M. A., Forma, F. M., Buenaga, R. F., Hofmann, K. J., Schultz, L. D., Gould, R. J. and Friedman, P. A. (1989). Gene 85: 511–516.

    Google Scholar 

  8. Schekman, R., Novick, P., ferro-Novick, S., Esmon, B., Hansen, W., Etcheverry, T., and Stevens, T. (1982).Rec. Adv. Yeast Mol. Biol., 1: 143–155.

    Google Scholar 

  9. Schultz, L. D., Hofmann, K. J., Mylin, L. M., Montgomery, D. L., Ellis, R. W. and Hopper, J. E. (1987b). Gene 61: 123–133.

    Google Scholar 

  10. Shaw, K. J., Frommer, B. R., Anagnost, J. A., Narula, S. and Leibowitz, P. (1988). DNA 7: 117–126.

    Google Scholar 

  11. St. John, T. P. and Davis, R. W. (1981). J. Mol. Biol. 152: 285–315.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cellular and Molecular Biology, Merck Sharp and Dohme Research Laboratories, 19486, West Point, PA

    C. E. Carty, K. J. Hofmann, P. M. Keller, B. J. Keech, R. Z. Maigetter & L. D. Schultz

  2. Department of Biological Chemistry, Merck Sharp and Dohme Research Laboratories, 19486, West Point, PA

    M. A. Polokoff, R. J. Lynch & R. J. Gould

Authors
  1. C. E. Carty
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. J. Hofmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. M. Keller
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. A. Polokoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. J. Lynch
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. B. J. Keech
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. R. J. Gould
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. R. Z. Maigetter
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. L. D. Schultz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Carty, C.E., Hofmann, K.J., Keller, P.M. et al. Regulated production of recombinant echistatin by yeast. Biotechnol Lett 12, 879–884 (1990). https://doi.org/10.1007/BF01022583

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01022583

Keywords

Search

Navigation