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Biotechnology Letters

, Volume 12, Issue 2, pp 81–86 | Cite as

Molecular cloning of non-polyadenylated nuclear hnRNAs

  • Jong Hwa Kim
  • Yang Do Choi
  • Si Myung Byun
Article
  • 19 Downloads

Summary

cDNA clones for the non-polyadenylated nuclear hnRNA of HeLa cell have been isolated. Nonpolyadenylated hnRNAs was purified by immunoaffinity chromatography and cDNA was synthesized with random hexamer as a primer. Radioactively labeled first strand cDNA was employed as a probe. One of the positive clones, S7S, shows sequence homology to human Alu sequence and another clone, W16W, shows a descrete Northern blot hybridization pattern to nuclear non-polyadenylated hnRNA. The RNA transcript which was encoded by a single copy gene was stable and relatively abundant.

Keywords

Chromatography HeLa Cell Copy Gene Bioorganic Chemistry Sequence Homology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Jong Hwa Kim
    • 1
  • Yang Do Choi
    • 2
  • Si Myung Byun
    • 1
  1. 1.Department of Biological Science and EngineeringKorea Advanced Institute of Science and TechnologySeoulKorea
  2. 2.Department of Agricultural ChemistrySeoul National UniversitySuwonKorea

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