Biotechnology Letters

, Volume 12, Issue 2, pp 81–86 | Cite as

Molecular cloning of non-polyadenylated nuclear hnRNAs

  • Jong Hwa Kim
  • Yang Do Choi
  • Si Myung Byun


cDNA clones for the non-polyadenylated nuclear hnRNA of HeLa cell have been isolated. Nonpolyadenylated hnRNAs was purified by immunoaffinity chromatography and cDNA was synthesized with random hexamer as a primer. Radioactively labeled first strand cDNA was employed as a probe. One of the positive clones, S7S, shows sequence homology to human Alu sequence and another clone, W16W, shows a descrete Northern blot hybridization pattern to nuclear non-polyadenylated hnRNA. The RNA transcript which was encoded by a single copy gene was stable and relatively abundant.


Chromatography HeLa Cell Copy Gene Bioorganic Chemistry Sequence Homology 
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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Jong Hwa Kim
    • 1
  • Yang Do Choi
    • 2
  • Si Myung Byun
    • 1
  1. 1.Department of Biological Science and EngineeringKorea Advanced Institute of Science and TechnologySeoulKorea
  2. 2.Department of Agricultural ChemistrySeoul National UniversitySuwonKorea

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