Biotechnology Letters

, Volume 16, Issue 10, pp 1081–1086 | Cite as

Characterization ofClostridium bifermentans and its biotransformation of 2,4,6-trinitrotoluene (TNT) and 1,3,5-triaza-1,3,5-trinitrocyclohexane (RDX)

  • K. M. Regan
  • R. L. Crawford


We have determined that an organism able to degrade both RDX and TNT in a pure culture is a strain ofClostridium bifermentans. The consortium from which this organism is derived also degrades these compounds, and we suspect thatC. bifermentans is also the responsible organism within that consortium. The bioconversion of RDX and TNT occurs under anaerobic conditions both in the consortium and in pure culture without the need of an added reductant. The presence of co-metabolites speeded these biotransformations.


Organic Chemistry Bioorganic Chemistry Anaerobic Condition Biotransformation Pure Culture 
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  1. Boopathy, R., and Kulpa, C. F. (1992).Current Microbiol. 25, 235–241.Google Scholar
  2. Boopathy, R., Kulpa, C. F., and M. Wilson (1993).Appl. Microbiol. Biotechnol. 39, 270–275.Google Scholar
  3. EPA (1990). Method 8330, SW-846.Google Scholar
  4. EPA (1994). Fact Sheet, March 1994, SuperFund Innovative Technology Evaluation (SITE).Google Scholar
  5. Funk, S. B., Roberts, D. J., Crawford, D. L., and Crawford, R. L. (1993).Appl. Environ. Microbiol. 59, 2171–2177.Google Scholar
  6. Kaplan, D. L., and Kaplan, A. M. (1982).Appl. Environ. Microbiol. 44, 757–760.Google Scholar
  7. McCormick, N. G., Cornell, J. H., and Kaplan A. M. (1981).Appl. Environ. Microbiol. 42(5), 817–823.Google Scholar
  8. McCormick, N. G., Feeherry, F. E. and Levinson, H. S. (1976).Appl. Environ. Microbiol. 31, 949–958.Google Scholar
  9. Myer and Miller (1988).Computer Appl. Biosci. 4, 11–17.Google Scholar
  10. Parrish, F. W. (1977).Appl. Environ. Microbiol. 34, 232–233.Google Scholar
  11. Preuss, A., Fimpel, J., and Diekert, G. (1993).Arch. Microbiol. 159, 345–353.Google Scholar
  12. Schackmann, A., and Muller, R. (1991).Appl. Microbiol. Biotechnol. 34, 809–813.Google Scholar
  13. Sutter, V. L. (1985). Susceptibility Testing of Anaerobes. In:Manual of Clinical Microbiology, E. H. Lennette, ed., 4th ed., Washington, D.C.: American Society for Microbiology.Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • K. M. Regan
    • 1
    • 2
  • R. L. Crawford
    • 1
    • 2
  1. 1.Department of Microbiology, Molecular Biology, and BiochemistryUniversity of IdahoMoscow
  2. 2.Center for Hazardous Waste Remediation ResearchUniversity of IdahoMoscow

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