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Direct reduction of NAD+ by electrochemical procedure and application of the regenerated NADH to enzyme reaction

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Summary

Enzymatically active NADH was formed from NAD+ directly in the electrochemical system by adopting an anion-charged membrane as a diaphragm and applying the less cathodic potential of −0.7V vs. Ag/AgCl electrode. The direct electrochemical procedure worked favorably as an NADH regenerator in the biochemical reaction system using D-lactate dehydrogenase which transforms pyruvate to D-lactate.

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Authors and Affiliations

  1. Dept. of Food Sci. and Technol., Chonbug National Univ., 560-756, Chonju, Korea

    Se-Eok Yun, Masahito Taya & Setsuji Tone

  2. Dept. of Chem. Eng., Osaka Univ., 560, Toyonaka, Japan

    Se-Eok Yun, Masahito Taya & Setsuji Tone

Authors
  1. Se-Eok Yun
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  2. Masahito Taya
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  3. Setsuji Tone
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Yun, SE., Taya, M. & Tone, S. Direct reduction of NAD+ by electrochemical procedure and application of the regenerated NADH to enzyme reaction. Biotechnol Lett 16, 1053–1058 (1994). https://doi.org/10.1007/BF01022402

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  • DOI: https://doi.org/10.1007/BF01022402

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