Skip to main content
SpringerLink
Log in

The influence of mRNA secondary structure on expression of endoglucanase inBacillus subtilis

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

A gene for endoglucanase ofBacillus subtilis has been inserted into a Bacillus expression plasmid containing a strong BJ27 promoter and a synthetic ribosome binding site. Secondary structure analysis of mRNA showed the presence of a strong hairpin loop burying the SD sequence and the initiation codon. Alteration of secondary structure at this site by deletion analysis revealed a correlation between endoglucanase expression and accessibility of the ribosome binding site. Elimination of secondary structures increased endoglucanase expression over five-fold to a level at which endoglucanase occupied 60% of total protein which was secreted into culture medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bradford, P. (1983).Anal. Biochem. 131, 333–336

    Google Scholar 

  • Horinouchi, S., and Weisblum, B. (1980).Proc. Natl. Acad. Sci. USA77, 7079–7083.

    Google Scholar 

  • Kawamura, F., and Doi, R.H. (1984).J. Bacteriol. 160, 442–444.

    Google Scholar 

  • Kim, J.H., and Pack, M.Y. (1993).Biotechnol. Lett. 15, 133–138.

    Google Scholar 

  • Lee, B.R., and Pack, M.Y. (1990).Biotechnol. Lett. 12, 925–930.

    Google Scholar 

  • Maniatis, T., Fritsch, E.F., and Sambrook, J. (1989).Molecular Cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

    Google Scholar 

  • Miller, G.L. (1959).Anal. Chem. 31, 426–428.

    Google Scholar 

  • Moran, C.P., Lang, N., LeGrice, S.F.J., Stephens, M., Sonenshine, A.L., Pero, J., and Losick, R. (1982).Mol. Gen. Genet. 186, 339–346.

    Google Scholar 

  • Shaw, W.N. (1975).Methods in Enzymol. 43, 737–755.

    Google Scholar 

  • Spizizen, J. (1958).Proc. Natl. Acad. Sci. USA.44, 1072–1078.

    Google Scholar 

  • Warburton, N., Boseley, P.G., and Porter, A.G. (1983).Nucleic Acids Res. 11, 5837–5854.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, Inje University, 621-749, Kimhae, Korea

    Baek-Rak Lee

  2. Department of Biotechnology, KAIST, 305-701, Taejon, Korea

    Jeong Hyun Kim & M. Y. Pack

Authors
  1. Baek-Rak Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jeong Hyun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Y. Pack
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, BR., Kim, J.H. & Pack, M.Y. The influence of mRNA secondary structure on expression of endoglucanase inBacillus subtilis . Biotechnol Lett 16, 1001–1006 (1994). https://doi.org/10.1007/BF01022392

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01022392

Keywords

Search

Navigation