Journal of Chemical Ecology

, Volume 12, Issue 8, pp 1765–1775 | Cite as

Elemental changes during sexual maturation inSchistosoma mansoni

  • Michael K. Shaw


Changes in the elemental composition ofSchistosoma mansoni in relation to age and sexual status are described, and studies on the structural localization of calcium in adult worms and the elemental composition of the tegument-subtegumental tissues are reviewed. Some preliminary results on the distribution of Ca2+-dependent ATPase activity in the tegument of worms from both mixed and single-sex infections are also presented.

Key words

Schistosoma mansoni inorganic metabolism elemental composition sexual status calcium localization X-ray probe microanalysis cryosections tegument subtegumental tissues Ca2+-dependent ATPase activity 


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  1. Ando, T., Fujimoto, K., Mayahara, H., andOgawa, K. 1981. A new one-step method for the histochemistry and cytochemistry of Ca-ATPase activity.Acta Histochem. Cytochem. 14:705–726.Google Scholar
  2. Barrett, J. 1981. Biochemistry of Parasitic Helminths. Macmillan, London.Google Scholar
  3. Bennett, J.L., Bueding, E., Timms, A.R., andEngstrom, R.G. 1969. Occurrence and levels of 5-hydroxytryptamine inSchistosoma mansoni.Mol. Pharmacol. 5:542–545.Google Scholar
  4. Brand, T. von. 1966. Biochemistry of Parasites. Academic Press, New York.Google Scholar
  5. Brand, T. von. 1979. Biochemistry and Physiology of Endoparasites. Elsevier/North Holland Biomedical Press, Amsterdam.Google Scholar
  6. Cesari, I.M., Simpson, A.J.G., andEvans, W.H. 1981. Properties of a series of tegumental membrane-bound phosphohydrolase activities ofSchistosoma mansoni.Biochem. J. 198:467–473.Google Scholar
  7. Chandler, J.A. 1978. The application of X-ray microanalysis in TEM to the study of ultrathin biological specimens—a review, pp. 35–93,in D.A. Erasmus (ed.). Electron Probe Microanalysis in Biology. Chapman and Hall, London.Google Scholar
  8. Coles, G.C. 1973. The metabolism of schistosomes: A review.Int. J. Biochem. 4:319–337.Google Scholar
  9. Coles, G.C. 1975. Fluke biochemistry—Fasciola andSchistosoma.Helminthol. Abstr. 44:147–162.Google Scholar
  10. Coles, G.C. 1984. Recent advances in schistosome biochemistry.Parasitology 89:603–637.Google Scholar
  11. Cornford, E.M., andOldendorf, W.H. 1979. Transintegumental uptake of metabolic substrates in male and female schistosomes.J. Parasitol. 69:357–363.Google Scholar
  12. Cornford, E.M., andHuot, M.E. 1981. Glucose transfer from male to female schistosomes.Science 213 1269–1271.Google Scholar
  13. Davies, T.W., andMorgan, A.J. 1976. The application of X-ray analysis in the transmission electron analytical microscope (T.E.A.M.) to the quantitative bulk analysis of biological microsamples.J. Microsc. 107:47–54.Google Scholar
  14. Dei-Cas, E., Dhainaut-Courtois, N., Dhainaut, A., andVernes, A. 1979. Ultrastructural localization of tritiated 5HT in adultSchistosoma mansoni. A preliminary report.Biol. Cell. 39:321–324.Google Scholar
  15. Donnelly, F.A., Appleton, C.C., andSchutte, C.H.J. 1984. The influence of salinity on the cercariae of three species ofSchistosoma.Int. J. Parasitol. 14:13–21.Google Scholar
  16. Dorsey, C.H., andStirewalt, M.A. 1977.Schistosoma mansoni: Localization of calcium-detecting reagents in electron-lucent areas of specific preacetabular gland granules.Z. Parasitenkd. 54:165–173.Google Scholar
  17. Dresden, M.H., andEdlin, E.M. 1975.Schistosoma mansoni: Calcium content of cercariae and its effects on protease activity in vitro.J. Parasitol. 61:398–402.Google Scholar
  18. Erasmus, D.A. 1973. A comparative study of the reproductive system of mature, immature and “unisexual” femaleSchistosoma mansoni.Parasitology 67:165–183.Google Scholar
  19. Erasmus, D.A. 1986. Structural and metabolic changes in femaleS. mansoni following male stimulation.J. Chem. Ecol. 12:1755–1764.Google Scholar
  20. Erasmus, D.A., andDavies, T.W. 1979.Schistosoma mansoni andS. haematobium: Calcium metabolism of the vitelline cell.Exp. Parasitol. 47:91–106.Google Scholar
  21. Erasmus, D.A., Popiel, I., andShaw, J.R. 1982. A comparative study of the vitelline cell inSchistosoma mansoni, S. haematobium, S. japonicum, andS. mattheei.Parasitology 84:283–287.Google Scholar
  22. Fetterer, R.H., Pax, R.A., andBennett, J.L. 1980.Schistosoma mansoni: Characterization of the electrical potential from the tegument of adult males.Exp. Parasitol. 49:353–365.Google Scholar
  23. Gönnert, R. 1955. Schistosomiasis studies II. Über die Eibildung derSchistosoma mansoni und das Schicksal der Eier im Wirtsorganismus.Z. Tropenmed. Parasitol. 6:33–52.Google Scholar
  24. Lawrence, J.D. 1973. The ingestion of red blood cells bySchistosoma mansoni.J. Parasitol. 59:60–63.Google Scholar
  25. Morgan, A.J. 1980. Preparation of specimens: Changes in chemical integrity, pp. 65–165,in M. A. Hayat (ed.). X-Ray Microanalysis in Biology. University Park Press, Baltimore, Maryland.Google Scholar
  26. Morgan, A.J. 1983. The electron microprobe analysis of sprayed microdroplets of solubilized biological tissues: A useful preliminary to localization studies.Scanning Electron Microsc. 1983(II):861–872.Google Scholar
  27. Morgan, A.J., Davies, T.W., andErasmus, D.A. 1978. Specimen preparation, pp. 94– D.A. Erasmus (ed.). Electron Probe Microanalysis in Biology. Chapman and Hall. London.Google Scholar
  28. Pappas, P.W., andRead, C.P. 1975. Membrane transport in helminth parasites: A review.Exp. Parasitol. 37:469–530.Google Scholar
  29. Podesta, R.B., andDean, L.L. 1982. Uptake of galactose by the epithelial syncytium ofSchistosoma mansoni.Mol. Biochem. Parasitol. 5:353–360.Google Scholar
  30. Podesta, R.B., andMcDiarmid, S.S. 1982. Enrichment and partial enzyme characterization of ATPase activity associated with the outward-facing membrane complex and inward-facing membrane of the surface epithelial syncytium ofSchistosoma mansoni.Mol. Biochem. Parasitol. 6:225–235.Google Scholar
  31. Shaw, J.R., andErasmus, D.A. 1981.Schistosoma mansoni: An examination of the reproductive status of females from single-sex infections.Parasitology 82:121–124.Google Scholar
  32. Shaw, M.K., andErasmus, D.A. 1982.Schistosoma mansoni: The presence and ultrastructure of vitelline cells in adult males.J. Helminthol. 56:51–53.Google Scholar
  33. Shaw, M.K., andErasmus, D.A. 1983.Schistosoma mansoni: Changes in elemental composition in relation to the age and sexual status of the worms.Parasitology 86:439–453.Google Scholar
  34. Shaw, M.K., andErasmus, D.A. 1984.Schistosoma mansoni: Electron probe X-ray microanalysis of the elemental composition of the tegument and subtegumental tissues of adult worms.Exp. Parasitol. 58:163–181.Google Scholar
  35. Shaw, M.K., Davies, T.W., andErasmus, D.A. 1982. The demonstration of calcium in the axonal granules of the peripheral nervous system of maleSchistosoma mansoni by X-ray microanalysis.Cell Tissue Res. 222:597–604.Google Scholar

Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • Michael K. Shaw
    • 1
  1. 1.Department of ZoologyUniversity CollegeCardiffWales UK

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