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The structural stability of an expression plasmid bearing a heterologous cloned gene depends on whether this gene is expressed or not

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Summary

During subculturing of recombinant Escherichia coli under conditions blocking cloned gene expression the plasmid was structurally stable for 200 generations. However, under conditions permitting foreign gene expression only 10% of plasmid containing cells had this gene functional after 100 generations.

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Authors and Affiliations

  1. Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Flemingovo 2, 166 37, Prague 6, Czechoslovakia

    F. Kaprálek & P. Ječmen

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  1. F. Kaprálek
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  2. P. Ječmen
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Kaprálek, F., Ječmen, P. The structural stability of an expression plasmid bearing a heterologous cloned gene depends on whether this gene is expressed or not. Biotechnol Lett 14, 251–256 (1992). https://doi.org/10.1007/BF01022319

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