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Cloning and expression inStreptomyces lividans of a chitosanase-encoding gene from the actinomyceteKitasatosporia N174 isolated from soil

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Summary

The chitosanase gene from a new soil isolate, the actinomyceteKitasatosporia N174, was cloned inStreptomyces lividans TK24. The enzyme expressed from the cloned gene had a molecular weight of approximately 29,500; an isoelectric point of 7.5 and was indistinguishable from the purified N174 chitosanase.

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Abbreviations

CHS:

chitosanase

chs :

chitosanase-encoding gene

kb:

kilobases

PAGE:

polyacrylamide gel electrophoresis

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. Groupe de Recherche en Biologie des Actinomycètes, Département de Biologie, Faculté des Sciences, Université de Sherbrooke, J1K 2R1, Sherbrooke, Québec, Canada

    Dominique Fink, Isabelle Boucher, François Denis & Ryszard Brzezinski

Authors
  1. Dominique Fink
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  2. Isabelle Boucher
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  3. François Denis
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  4. Ryszard Brzezinski
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Fink, D., Boucher, I., Denis, F. et al. Cloning and expression inStreptomyces lividans of a chitosanase-encoding gene from the actinomyceteKitasatosporia N174 isolated from soil. Biotechnol Lett 13, 845–850 (1991). https://doi.org/10.1007/BF01022084

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