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Improvement of the catharanthine productivity in hairy root cultures ofCatharanthus roseus by using monosaccharides as a carbon source

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Summary

Sucrose, glucose, and fructose as carbon sources in culture medium were assessed in hairy root cultures ofCatharanthus roseus. The cultures preferentially consumed sucrose, resulting in about 40% (dry wt) higher growth rate. However, fructose enhanced the cathranthine yield about two-fold. The elevated yield was not seemingly ascribed to the higher osmolarity per unit weight of fructose than sucrose. A two stage culture using sucrose (1st) and fructose (2nd) improved volumetric yields of catharanthine about two-fold, i.e. 41 mg/l.

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Authors and Affiliations

  1. Plant Cell Biology Laboratory, Genetic Engineering Research Institute, Korea Institute of Science and Technology, P.O. Box 17, 305-606, Taedok Science Town, Taejon, Korea

    K. H. Jung, S. S. Kwak, S. W. Kim & J. R. Liu

  2. Biochemical Engineering Major, Seoul National University, 151-742, Seoul, Korea

    K. H. Jung & C. Y. Choi

  3. Department of Chemical Engineering, KAIST, Kusung-dong, Yusung-gu, Taejon, Korea

    H. Lee

Authors
  1. K. H. Jung
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  2. S. S. Kwak
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  3. S. W. Kim
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  4. H. Lee
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  5. C. Y. Choi
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  6. J. R. Liu
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Jung, K.H., Kwak, S.S., Kim, S.W. et al. Improvement of the catharanthine productivity in hairy root cultures ofCatharanthus roseus by using monosaccharides as a carbon source. Biotechnol Lett 14, 695–700 (1992). https://doi.org/10.1007/BF01021645

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  • DOI: https://doi.org/10.1007/BF01021645

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