Biotechnology Letters

, Volume 14, Issue 5, pp 363–366 | Cite as

Enantioselective oxidation of mandelic acid using a phenylmalonate metabolizing pathway of a soil bacteriumAlcaligenesbronchisepticus KU 1201

  • Kenji Miyamoto
  • Hiromichi Ohta
Article

Summary

The metabolic pathway of phenylmalonic acid byAlcaligenesbronchisepticus KU1201, which catalyzes the asymmetric decarboxylation of α-aryl-α-methylmalonic acids to α-arylpropionic acids, is demonstrated. Enantioselective oxidation of mandelic acid was investigated using the metabolizing pathway in this strain. Incubation of (±)-mandelic acid withA.bronchisepticus afforded optically pure (R)-one accompanied by benzoylformic acid and benzoic acid. This enzymatic oxidation is also applicable to various substituted mandelic acids.

Keywords

Oxidation Organic Chemistry Metabolic Pathway Bioorganic Chemistry Benzoic Acid 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Kenji Miyamoto
    • 1
  • Hiromichi Ohta
    • 1
  1. 1.Department of Chemistry, Faculty of Science and TechnologyKeio UniversityYokohamaJapan

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