Journal of Chemical Ecology

, Volume 16, Issue 4, pp 1371–1383 | Cite as

Effects of soil nitrogen level on ferulic acid inhibition of cucumber leaf expansion

  • Kristofer Klein
  • Udo Blum
Article

Abstract

It has been suggested that the allelopathic activity of phenolic acids should be primarily important in soils of low fertility. If this is true, then plant growth inhibition by phenolic acids may be unimportant in managed agricultural soils. The objective of this study was to determine how soil nitrogen (N) level might modify phenolic acid inhibition of growth. Cucumber seedlings (Cucumis sativus cv Early Green Cluster) grown in containers in growth chambers under varying N levels (5, 10, 15, 20, and 25 μg N/g soil) in Portsmouth B,-horizon soil material were treated with ferulic acid (0 or 10 μg/g soil). Nitrogen and ferulic acid (FA) were applied every other day to the soil surface. The amount of FA in the soil solution declined with depth in the containers. A more rapid disappearance of FA from the soil solution was observed for the last FA treatment (0% recovered after 10 hr on day 23) than the first treatment (44% recovered after 10 hr on day 13). Both low N (5 μg N/g soil) and FA treatments reduced shoot dry weight, the mean absolute (AGR) and the mean relative (RGR) rates of leaf expansion, and increased the root-shoot ratio. High N treatments reduced shoot dry weight and the AGR. Ferulic acid inhibited cucumber seedling growth over a range of N concentrations, suggesting that the allelopathic activity of phenolic acids may be important in both nutrient limiting and nonlimiting soils for some species.

Key words

Allelopathy ferulic acid Cucumis sativus leaf expansion nitrogen levels growth inhibition 

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References

  1. Bates-Smith, E.C. 1956. The commoner phenolic constituents of plants and their systematic distribution.Proc. R. Dublin Sci. Soc. 27:165–176.Google Scholar
  2. Blum, U., andDalton, B.R. 1985. Effects of ferulic acid, an allelopathic compound, on leaf expansion of cucumber seedlings grown in nutrient culture.J. Chem. Ecol. 11:279–301.Google Scholar
  3. Blum, U., andRebbeck, J. 1988. The inhibition and recovery of cucumber roots given multiple treatments of ferulic acid in nutrient culture.J. Chem. Ecol. 15:917–928.Google Scholar
  4. Blum, U., andShafer, S.R. 1988. Enrichment of Portsmouth soil with simple phenolic acids: Effects on microbial populations and concentrations of water extractable phenolic acids in soil.Soil Biol. Kochem. 20:793–800.Google Scholar
  5. Blum, U., Dalton, B.R., andShann, J.R. 1985a. Effects of various mixtures of ferulic acid and some of its microbial metabolic products on cucumber leaf expansion and dry matter in nutrient culture.J. Chem. Ecol 11:619–641.Google Scholar
  6. Blum, U., Dalton, B.R., andShann, J.R. 1985b. The effects of ferulic andp-coumaric acids in nutrient culture on cucumber leaf expansion as influenced by pH.J. Chem. Ecol. 11:1567–1582.Google Scholar
  7. Blum, U., Weed, S.B., andDalton, B.R. 1987. Influence of various soil factors on the effects of ferulic acid on leaf expansion of cucumber seedlings.Plant Soil 98:111–130.Google Scholar
  8. Dalton, B.R. 1984. The behavior of ferulic acid in soils varying in pH, minerology and organic matter content. MS thesis. N.C.S.U. Raleigh, North Carolina.Google Scholar
  9. Dalton, B.R., Blum, U., andWeed, S.B. 1983. Allelopathic substances in ecosystems: Effectiveness of sterile soil components in altering recovery of ferulic acid.J. Chem. Ecol. 9:1185–1201.Google Scholar
  10. Einhellig, F.A., andEckrich, P.C. 1984. Interactions of temperature and ferulic acid stress on grain sorghum and soybeans.J. Chem. Ecol. 10:161–169.Google Scholar
  11. Glass, A.M. 1973. Influence of phenolic acids on ion uptake. I. Inhibition of phosphate uptake.Plant Physiol. 51:1037–1041.Google Scholar
  12. Glass, A.M. 1974. Influence of phenolic acids on ion uptake. III. Inhibition of potassium absorption.J. Exp. Bot. 89:1104–1113.Google Scholar
  13. Glass, A.M., andDunlop, J. 1974. Influence of phenolic acids on ion uptake. IV. Depolarization of membrane potentials.Plant Physiol. 54:855–858.Google Scholar
  14. Haider, K., andMartin, J.P. 1975. Decomposition of specifically carbon-14 labeled benzoic and cinnamic acid derivatives in soil.Soil Sci. Soc. Am. Proc. 39:657–662.Google Scholar
  15. Hall, A.B., Blum, U.B., andFites, R.C. 1983. Stress modification ofHelianthus annus L. debris on seedling biomass production ofAmaranthus retroflexus L.J. Chem. Ecol. 9:1213–1222.Google Scholar
  16. Harper, J.R., andBalke, N.E. 1981. Characterization of the inhibition of K+ absorbtion in oat roots by salycilic acid.Plant Physiol. 68:1349–1353.Google Scholar
  17. Klein, K., andBlum, U. 1990. Inhibition of cucumber leaf expansion by ferulic acid in split-root experiments.J. Chem. Ecol. 16:455–463.Google Scholar
  18. Liebl, R., andWorsham, A.D. 1983. Inhibition of pitted morning glory (Ipomea lacunosa L.) and certain other weed species by phytotoxic components of wheat (Triticum aestivum L.) straw.J. Chem. Ecol. 9:1027–1043.Google Scholar
  19. Lynch, J.M. 1985. Allelopathy involving microorganisms: Case histories from the United Kingdom.A.C.S. Symp. Ser. 330:44–52.Google Scholar
  20. Martin, J.P., andHaider, K. 1976. Decomposition of specifically carbon-14-labeled ferulic acid: Free and linked into hummic acid-type polymers.Soil Sci. Soc. Am. J. 40:377–380.Google Scholar
  21. Martin, J.P., Haider, K., andWolf, D. 1972. Synthesis of phenols and phenolic polymers byHendersonula toruloidea in relation to humic acid formation.Soil Sci. Soc. Am. Proc. 36:311–315.Google Scholar
  22. Patrick, Z.A., Toussoun, T.A., andKoch, L.W. 1964. Effect of crop residue decomposition products on plant roots.Annu. Rev. Phytopathol. 2:267–292.Google Scholar
  23. Radford, D.T. 1967. Growth analysis formulae- their use and abuse.Crop Sci. 7:171–175.Google Scholar
  24. Rice, E.L. 1984. Allelopathy, 2nd ed. Academic Press, Orlando, Florida.Google Scholar
  25. Shann, J.R., andBlum, U. 1987. The uptake of ferulic andp-coumaric acids byCucumis sativus.Phytochemistry 26:2959–2964.Google Scholar
  26. Stowe, L.G., andOsborn, A. 1980. The influence of nitrogen and phosphorus levels on the phytotoxicity of phenolic compounds.Can. J. Bot. 58:1149–1153.Google Scholar
  27. Turner, J.A., andRice, E.L. 1975. Microbial decomposition of ferulic acid in soil.J. Chem. Ecol. 1:41–58.Google Scholar
  28. Vaughn, D., Sparling, G.P., andOrd, B.C. 1983. Amelioration of the phytotoxicity of phenolic acids by some soil microbes.Soil Biol. Biochem. 15:613–614.Google Scholar
  29. Whitehead, D.C. 1964. Identification ofp- hydroxybenzoic, vanillicp-coumaric and ferulic acids.Nature 202:417–418.Google Scholar
  30. Whitehead, D.C., Dibb, H., andHartley, R.D. 1981. Extradant pH and the release of phenolic compounds from soils, plant roots and leaf litter.Soil Biol. Biochem. 13:343–348.Google Scholar

Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Kristofer Klein
    • 1
  • Udo Blum
    • 1
  1. 1.Department of BotanyNorth Carolina State UniversityRaleigh

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