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Biotechnology Letters

, Volume 16, Issue 11, pp 1125–1130 | Cite as

The gene (lacA) encoding galactoside acetyltransferase fromLactococcus lactis

  • Hugh G. Griffin
  • Michael J. Gasson
Article

Summary

This paper reports the cloning and characterization of a gene encoding galactoside acetyltransferase from a strain ofLactococcus lactis. AP stI library ofL. lactis strain ATCC7962 DNA was constructed in plasmid pUC18. A clone harbouring a 10 kbp DNA fragment containing part of thelac operon was isolated using a labelled probe generated by PCR. DNA sequence analysis revealed the presence of a gene encoding a protein with 64.5% similarity to the galactoside acetyltransferase fromEscherichia coli. The codon usage pattern of this gene was not typical of lactococcal genes. The lactococcallac operon organization appears to be different to that of other organisms.

Keywords

Codon Organic Chemistry Sequence Analysis Lactis Bioorganic Chemistry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Hugh G. Griffin
    • 1
  • Michael J. Gasson
    • 1
  1. 1.Institute of Food ResearchNorwich Research ParkNorwichUK

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