Effects of initial medium pH on growth and metabolism ofCatharanthus roseus hairy root cultures —A study with31P and13C NMR spectroscopy
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Hairy root cultures ofCatharanthus roseus were grown for 26 days in half-strength Gamborg's B5 liquid medium at different initial pH values of 4.2, 5.7, 6.5, and 7.3. Maximum growth was obtained for cultures with an initial medium pH 6.5. The lowest growth rate was found in cultures at initial pH values of 4.2 and 7.3. Roots in cultures at initial pH of 4.2 had a thickened and stunted morphology in contrast to the other cultures. Also, cultures at initial medium pH of 4.2 exhibited an increase in medium pH in the first few days instead of the characteristic acidification. All cultures maintained a cytoplasmic pH of 7.4 throughout the growth cycle. However, vacuolar pH was 5.1–5.2 in cultures of initial pH 4.2, as opposed to 5.4–5.5 for other cultures. Sucrose was hydrolyzed completely to glucose and fructose by day 26 except for cultures at initial pH of 7.3. Glucose was the preferred substrate throughout the growth cycle for cultures with initial pH values of 7.3 and 6.5, after day 20 for an initial pH of 5.7, and after day 26 for pH 4.2.
KeywordsSucrose Fructose Liquid Medium Bioorganic Chemistry Maximum Growth
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