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Pseudomonas cepacia lipase: Studies on aggregation, purification and on the cleavage of olive oil

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Summary

To purify the extracellular lipase ofPseudomonas cepacia DSM 50181 and to disintegrate molecular aggregation, the presence of i-propanol (70% per volume) was necessary. The molecular weight was determined to be 33,000 D. Concerning the hydrolytic activity of the pure enzyme towards olive oil additional free fatty acids inhibited the reaction.

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Authors and Affiliations

  1. Institute of Biochemistry and Biotechnology, Technical University, D-3300, Braunschweig, Germany

    Armin Dünhaupt, Siegmund Lang & Fritz Wagner

Authors
  1. Armin Dünhaupt
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  2. Siegmund Lang
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  3. Fritz Wagner
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Dünhaupt, A., Lang, S. & Wagner, F. Pseudomonas cepacia lipase: Studies on aggregation, purification and on the cleavage of olive oil. Biotechnol Lett 14, 953–958 (1992). https://doi.org/10.1007/BF01020636

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  • DOI: https://doi.org/10.1007/BF01020636

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