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Effect of the enzyme form on the activity, stability and enantioselectivity of lipoprotein lipase in toluene

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Summary

The properties of various forms of lipoprotein lipase (powder, adsorbed onto Celite, covalently linked to PEG, with additives) in toluene were investigated. The form of the enzyme dramatically influenced the activity and stability of the enzyme with the highest activity obtained with PEG-lipase and the highest stability with Celite-immobilized lipase. By contrast, the enantioselectivity was only marginally affected.

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References

  • Cambou, B., Klibanov, A.M. (1984).J. Am. Chem. Soc. 106, 2687–2692.

    Google Scholar 

  • Carrea, G. (1984).Trends Biotechnol. 2, 102–106.

    Google Scholar 

  • Chen, C.-S., Sih, C.J. (1989).Angew. Chem. Int. Ed. Engl. 28, 695–707.

    Google Scholar 

  • De Amici, M., De Micheli, C., Carrea, G., Spezia, S. (1989).J. Org. Chem. 54, 2646–2650.

    Google Scholar 

  • Gaertner, H., Puigserver, A. (1989).Eur. J. Biochem. 181, 207–213.

    Google Scholar 

  • Habeeb, A. (1966).Anal. Biochem. 14, 328–336.

    Google Scholar 

  • Hirano, Y., Terai, T., Goto, K., Nakajima, A. (1991).Agric. Biol. Chem. 55, 2461–2465.

    Google Scholar 

  • Inada, Y., Takahashi, K., Yoshimoto, T., Ajima, A., Matsushima, A., Saito, Y. (1986).Trends Biotechnol. 4, 190–194.

    Google Scholar 

  • Khan, s., Halling, P.J., Bosley, J.A., Clark, A.H., Peilow, A.D., Pelan, E.G., Rowlands, D.W. (1992).Enzyme Microb. Technol. 14, 96–100.

    Google Scholar 

  • Klibanov, A.M. (1990).Acc. Chem. Res. 23, 114–120.

    Google Scholar 

  • Ottolina, G., Carrea, G., Riva, S. (1990).J. Org. Chem. 55, 2366–2369.

    Google Scholar 

  • Reslow, M., Adlercreutz, P., Mattiasson, B. (1988).Eur. J. Biochem. 172, 573–578.

    Google Scholar 

  • Riva, S., Klibanov, A.M. (1988).J. Am. Chem. Soc. 110 3291–3295.

    Google Scholar 

  • Sartore, L., Caliceti, P., Schiavon, O., Monfardini, C., Veronese, F.M. (1991).Appl. Biochem. Biotechnol. 31, 213–222.

    Google Scholar 

  • Secundo, F., Riva, S., Carrea, G. (1992).Tetrahedron Asymmetry 3, 267–280.

    Google Scholar 

  • Yamane, T., Ichiryu, T., Nagata, M., Ueno, A., Shimizu, S. (1990).Biotechnol. Bioeng. 36, 1063–1069.

    Google Scholar 

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Authors and Affiliations

  1. Istituto di Chimica degli Ormoni, C.N.R., Via Mario Bianco 9, 20131, Milano, Italy

    G. Ottolina, G. Carrea & S. Riva

  2. Dipartimento di Scienze Farmaceutiche, Via Marzolo 5, 35131, Padova, Italy

    L. Sartore & F. M. Veronese

Authors
  1. G. Ottolina
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  2. G. Carrea
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  3. S. Riva
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  4. L. Sartore
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  5. F. M. Veronese
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Ottolina, G., Carrea, G., Riva, S. et al. Effect of the enzyme form on the activity, stability and enantioselectivity of lipoprotein lipase in toluene. Biotechnol Lett 14, 947–952 (1992). https://doi.org/10.1007/BF01020635

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  • DOI: https://doi.org/10.1007/BF01020635

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