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One-step synthesis of Gly-Gly-PheNH2 from N-unprotected amino acid derivatives by papain in one-phase liquid media

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Summary

Papain was able to catalyze the one-step synthesis of Gly-Gly-PheNH2, from N-unprotected amino acid derivatives. Maximum synthetic activity was obtained for a pH value of 6.5 and for a ]PheNH2]/[Gly-GlyOEt] ratio of 6. The presence of an organic cosolvent, such as ethylene glycol, influenced the synthetic activity. Synthetic yield was higher than 65% for a 12.5 M cosolvent concentration.

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Authors and Affiliations

  1. Departmento de Bioquímica y Biología Molecular B e Inmunología. Facultad de Química, Universidad de Murcia, Ap 4021, Murcia, España

    Pedro Lozano, José L. Iborra & Arturo Manjón

  2. Department de Genie Biochimique et Alimentaire, INSA. UA-CNRS 544, Av Rangueil, 31077, Toulouse, France

    Didier Combes

Authors
  1. Pedro Lozano
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  2. José L. Iborra
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  3. Arturo Manjón
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  4. Didier Combes
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Lozano, P., Iborra, J.L., Manjón, A. et al. One-step synthesis of Gly-Gly-PheNH2 from N-unprotected amino acid derivatives by papain in one-phase liquid media. Biotechnol Lett 14, 933–936 (1992). https://doi.org/10.1007/BF01020632

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  • DOI: https://doi.org/10.1007/BF01020632

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