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Production of acetylcholine receptors from TE671 cells grown on microcarriers

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Summary

When grown in DMEM supplemented with 10 % fetal calf serum and using Cytodex 3® as microcarriers, TE671 cells entering the stationary phase optimally expressed acetylcholine receptors. These, receptors could be conveniently extracted from cell-saturated, microbeads or monodispersed cells obtained by trypsinization of microbeads. Typically, a 500 ml-batch gave 6–7 pmol of receptors which could be used as antigen to assay anti-acetylcholine receptor antibodies in the sera of myasthenic patients.

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References

  • Bradford, M. (1978).Anal. Biochem. 72, 248–254.

    Google Scholar 

  • Braun, S., Askanas, V., Ibrahim, E. and Engel, W.K. (1991)Neurology 41, 154.

    Google Scholar 

  • Braun, S., Transchant, C., Vilquin, J.-T., Labouret, P., Warter, J.-M., and Poindron, P. (1989).J. Neurol. Sci. 92, 119–131.

    Google Scholar 

  • Harcourt, G., and Jermy, A. (1987)Immunol., Today 8, 319–321.

    Google Scholar 

  • Kaplan, I., Blakely, B.T., Pavlath, G.C., Travis, M. and Blau, H. (1990).Proc. Natl. Acad. Sci. USA 87, 8100–8104.

    Google Scholar 

  • Lukas, R.J. (1986).J. Neurochem. 46, 1936–1941.

    Google Scholar 

  • McAllister, R.M., Isaacs, H., Rongey, R., Peer, M., Au, W., Soukup, S.W. and Gardner, M.B. (1977).Int. J. Cancer 20, 206–212.

    Google Scholar 

  • Schoepfer, R., Luther, M. and Lindstrom, J. (1988).FEBS Lett. 226, 235–240.

    Google Scholar 

  • Stratton, M.R., Reeves, B.R. and Cooper, C.S. (1989).Nature 337, 311–312.

    Google Scholar 

  • Syapin, P.J., Salvaterra, P.M. and Engelhardt, J.K. (1982).Brain Res. 231, 365–377.

    Google Scholar 

  • Vilquin, J.-T., Braun, S., Labouret, P., Zuber, G., Tranchant, C., Poindron, P. and Warter, J.-M. (1992).J. Neurosci. Res. 31, 285–293.

    Google Scholar 

  • Walker, R., Vincent, A. and Newsom-Davis, J. (1988).J. Neuroimmunol. 19, 149–157.

    Google Scholar 

  • Waymouth, C. (1982). Methods for obtaining cells in suspension from animal tissues. In:Cell separation. Methods and selected applications, T.G. Pretlow and T.P. Preflow, eds.. vol. 1, pp. 1–29 New York, Ac. Press.

    Google Scholar 

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Authors and Affiliations

  1. Centre de Recherches Pharmaceutiques, Département d'Immunologie Immunopharmacologie et Pathologie [Equipes de Biologie Cellulaire et de Neurologie Clinique], Université Louis Pasteur, BP 24, 67401, Illkirch Cedex, France

    Jean-Thomas Vilquin, Philippe Kennel, Paul Fonteneau, Jean-Marie Warter & Philippe Poindron

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  1. Jean-Thomas Vilquin
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  2. Philippe Kennel
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  3. Paul Fonteneau
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  4. Jean-Marie Warter
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  5. Philippe Poindron
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Vilquin, JT., Kennel, P., Fonteneau, P. et al. Production of acetylcholine receptors from TE671 cells grown on microcarriers. Biotechnol Lett 14, 875–880 (1992). https://doi.org/10.1007/BF01020621

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