Advertisement

Endurance training decreases the alkaline proteolytic activity in mouse skeletal muscles

  • A. Salminen
  • M. Kihlström
  • H. Kainulainen
  • T. Takala
  • V. Vihko
Article

Summary

Alkaline and myofibrillar protease activities of rectus femoris, soleus, and tibialis anterior muscles and the pooled sample of gastrocnemius and plantaris muscles were analyzed in male NMRI-mice during a running-training program of 3, 10, or 20 daily 1-h sessions. The activity of citrate synthase increased during the endurance training, reflecting the increased oxidative capacity of skeletal muscles. The activities of alkaline and myofibrillar proteases continually decreased in the course of the training program in all muscles studied. Instead, the activity ofΒ-glucuronidase (a marker of lysosomal hydrolases) increased in all muscles. The highest activities were observed at the beginning of the training program. Present results, together with our earlier observations, show that the type of training, running as opposed to swimming, modulates the training responses in alkaline protease activities. Further, diverse adaptations in the activities of alkaline proteases and a lysosomal hydrolase suggest differences in the function of different proteolytic systems.

Key words

Endurance training Muscles Alkaline proteinases Mouse 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ballard FJ (1977) Intracellular protein degradation. Essays Biochem 13: 1–37Google Scholar
  2. Brinkworth RI, Masters CJ (1978) The turnover of lactate dehydrogenase in skeletal muscle of the mouse. Influence of fibre type and exercise regimen. Biochim Biophys Acta 540: 1–12Google Scholar
  3. Dahlmann B, Reinauer H (1978) Purification and some properties of an alkaline proteinase from rat skeletal muscle. Biochem J 171: 803–810Google Scholar
  4. Dahlmann B, Schroeter C, Herbertz L, Reinauer H (1979) Myofibrillar protein degradation and muscle proteinases in normal and diabetic rats. Biochem Med 21: 33–39Google Scholar
  5. Dahlmann B, Widjaja A, Reinauer H (1981) Antagonistic effects of endurance training and testosterone on alkaline proteolytic activity in rat skeletal muscles. Eur J Appl Physiol 46: 229–235Google Scholar
  6. Dayton WR, Reville WJ, Goll DE, Stromer MH (1976) A Ca2+-activated protease possibly involved in myofibrillar protein turnover. Partial characterization of the purified enzyme. Biochemistry 15: 2159–2167Google Scholar
  7. Dohm GL, Beecher GR, Hecker AL, Puente FR, Klain GJ, Askew EW (1977) Changes in protein synthesis in rats in response to endurance training. Life Sci 21: 189–198Google Scholar
  8. Drabikowski W, Gorecka A, Jakubiec-Puka A (1977) Endogenous proteinases in vertebrate skeletal muscle. Int J Biochem 8: 61–71Google Scholar
  9. Edmunds T, Pennington RJT (1981) Mast cell origin of myofibrillar protease of rat skeletal and heart muscle. Biochim Biophys Acta 661: 28–31Google Scholar
  10. Howald H (1982) Training-induced morphological and functional changes in skeletal muscle. Int J Sports Med 3: 1–12Google Scholar
  11. Jaweed MM, Gordon EE, Herbison GJ, Kowalski K (1974) Endurance and strengthening exercise adaptations: I. Protein changes in skeletal muscles. Arch Phys Med Rehabil 55: 513–517Google Scholar
  12. Kar NC, Pearson CM (1978) Muscular dystrophy and activation of proteinases. Muscle Nerve 1: 308–313Google Scholar
  13. Katunuma N, Kominami E (1977) Group-specific proteinases for apoproteins of pyridoxal enzymes. In: Barrett AJ (ed) Proteinases in mammalian cells and tissues. North-Holland, Amsterdam, pp 151–180Google Scholar
  14. Kelly FJ, Goldspink DF (1982) The differing responses of four muscle types to dexamethasone treatment in the rat. Biochem J 208: 147–151Google Scholar
  15. Lernau OZ, Nissan S, Neufeld B, Mayer M (1980) Myofibrillar protease activity in muscle tissue from patients in catabolic conditions. Eur J Clin Invest 10: 357–361Google Scholar
  16. Lojda Z, Gutman E (1976) Histochemistry of some acid hydrolases in striated muscle of the rat. Histochemistry 49: 337–342Google Scholar
  17. Mayer M, Amin R, Shafrir E (1974) Rat myofibrillar protease: enzyme properties and adaptive changes in conditions of muscle protein degradation. Arch Biochem Biophys 161: 20–25Google Scholar
  18. Mayer M, Amin R, Milholland RJ, Rosen F (1976) Possible significance of myofibrillar protease in muscle catabolism. Enzyme activity in dystrophic, tumor-bearing, and glucocorticoid-treated animals. Exp Mol Pathol 25: 9–19Google Scholar
  19. McElligott MA, Bird JWC (1981) Muscle proteolytic enzyme activities in diabetic rats. Am J Physiol 241: E378–384Google Scholar
  20. Murakami U, Uchida K (1978) Purification and characterization of a myosin-cleaving protease from rat heart myofibrils. Biochim Biophys Acta 525: 219–229Google Scholar
  21. Pennington RJT (1977) Proteinases of muscle. In: Barrett AJ (ed) Proteinases in mammalian cells and tissues. North-Holland, Amsterdam, pp 515–543Google Scholar
  22. Rogozkin VA (1976) The effect of the number of daily training sessions on skeletal muscle protein synthesis. Med Sci Sports 8: 223–225Google Scholar
  23. Salminen A, Vihko V (1980) Acid proteolytic capacity in mouse cardiac and skeletal muscles after prolonged submaximal exercise. Pfluegers Arch 389: 17–20Google Scholar
  24. Salminen A, Vihko V (1981) Effects of age and prolonged running on proteolytic capacity in mouse cardiac and skeletal muscles. Acta Physiol Scand 112: 89–95Google Scholar
  25. Salminen A, Vihko V (1983) Exercise myopathy: selectively enhanced proteolytic capacity in rat skeletal muscle after prolonged running. Exp Mol Pathol 38: 61–68Google Scholar
  26. Salminen A, Komulainen J, AhomÄki E, Kainulainen H, Takala T, Vihko V (1983) Effects of endurance training on alkaline protease activities in rat skeletal muscles. Acta Physiol Scand 119: 261–265Google Scholar
  27. Seene T, Viru A (1982) The catabolic effect of glucocorticoids on different types of skeletal muscle fibers and its dependence upon muscle activity and interaction with anabolic steroids. J Steroid Biochem 16: 349–352Google Scholar
  28. Tapscott EB Jr, Kasperek GJ, Dohm GL (1982) Effect of training on muscle protein turnover in male and female rats. Biochem Med 27: 254–259Google Scholar
  29. Tomas FM, Munro HN, Young VR (1979) Effect of glucocorticoid administration on the rate of muscle protein breakdown in vivo in rats, as measured by urinary excretion of N-methylhistidine. Biochem J 178: 139–146Google Scholar
  30. Vihko V, Salminen A, RantamÄki J (1978a) Acid hydrolase activity in red and white skeletal muscle of mice during a two-week period following exhausting exercise. Pfluegers Arch 378: 99–106Google Scholar
  31. Vihko V, Salminen A, RantamÄki J (1978b) Oxidative and lysosomal capacity in skeletal muscle of mice after endurance training of different intensities. Acta Physiol Scand 104: 74–81Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • A. Salminen
    • 1
  • M. Kihlström
    • 1
  • H. Kainulainen
    • 1
  • T. Takala
    • 2
  • V. Vihko
    • 1
  1. 1.Division of Muscle Research, Department of Cell BiologyUniversity of JyvÄskylÄJyvÄskylÄ 10
  2. 2.Department of Clinical ChemistryUniversity of OuluOulu 22Finland

Personalised recommendations