Journal of Chemical Ecology

, Volume 15, Issue 4, pp 1219–1229 | Cite as

Host egg kairomones essential for egg-larval parasitoid,Ascogaster reticulatus Watanabe (Hymenoptera: Braconidae)

II. Identification of internal kairomone
  • Yooichi Kainoh
  • Sadahiro Tatsuki
  • Hajime Sugie
  • Yoshio Tamaki
Article

Abstract

Several components of an internal kairomone were identified inside eggs of the host,Adoxophyes sp. (Lepidoptere: Tortricidae), that releases egg deposition of the egg-larval parasitoid,Ascogaster reticulatus Watanabe (Hymenoptera: Braconidae). Pupal hemolymph with the same activity as an internal host egg kairomone was used as a convenient test sample. Heat-treated pupal hemolymph was chromatographed on a Sephadex G-25 column. Each fraction was bioassayed and reacted with ninhydrin. The active fractions were ninhydrin-positive. Each fraction was placed onto an araino acid analyzer, which showed that the amino acids were most abundant in active fractions. Among 22 amino acids, alanine, arginine, glycine, histidine, isoleucine, leucine, methionine, proline, serine, tryptophan, and valine were active. The mixture of these active amino acids was as active as the egg-mass homogenate at the same ratio and concentration, suggesting that the most important component as the kairomone in a host egg is the mixture of several amino acids.

Key words

Kairomone oviposition stimulant amino acid parasitoid Ascogaster reticulatus Hymenoptera Braconidae Adoxophyes sp. Lepidoptera Tortricidae 

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References

  1. Arthur, A.P. 1981. Host acceptance by parasitoids, pp. 97–120,in D.A. Nordlund, R.L. Jones, and W.J. Lewis (eds.). Semiochemicals—Their Role in Pest Control, Wiley-Interscience, New York.Google Scholar
  2. Arthur, A.P., Heodekar, B.M., andRollins, L. 1969. Component of the host haemolymph that induces oviposition in a parasitic insect.Nature 223:966–967.Google Scholar
  3. Arthur, A.P., Hegdekar, B.M., andBatsch, W.W. 1972. A chemically defined, synthetic medium that induces oviposition in the parasiteItoplectis conquisitor (Hymenoptera: Ichneumonidae).Can. Entomol. 104:1251–1258.Google Scholar
  4. Du, J.W., Fu, W.J., Ding, D.C., Qui, H.G., Dai, X., Xu, S. 1982. Isolation and identification of chemical structure of the kairomones influencing oviposition behavior ofTetrastichus schoenobii Fernere.Kexue Tongbao 27:766–770.Google Scholar
  5. Hegdekar, B.M., andArthur, A.P. 1973. Host hemolymph chemicals that induce oviposition in the parasiteItoplectis conquisitor (Hymenoptera: Ichneumonidae).Can. Entomol. 105:787–793.Google Scholar
  6. Hu, J.S., andChen, C.M. 1987. A study on the host-searching kairomone ofApanteles cypris Nixon.Acta Entomol. Sin. 30:31–40.Google Scholar
  7. Kainoh, Y. 1986. Mating behavior ofAscogaster reticulatus Watanabe (Hymenoptera: Braconidae), an egg-larval parasitoid of the smaller tea tortrix moth,Adoxophyes sp. (Lepidoptera: Tortricidae). I. Diel patterns of emergence and mating, and some conditions formating.Appl. Entomol. Zool. 21:1–7.Google Scholar
  8. Kainoh, Y., andTamaki, Y. 1982. Searching behavior and oviposition of the egg-larval parasitoid,Ascogaster reticulatus Watanabe (Hymenoptera: Braconidae).Appl. Entomol. Zool. 17:194–206.Google Scholar
  9. Kainoh, Y., andTatsuki, S. 1988. Host egg kairomones essential for the egg-larval parasitoid,Ascogaster reticulatus Watanabe (Hymenoptera: Braconidae). I. Internal and external kairomones.J. Chem. Ecol. 14:1475–1484.Google Scholar
  10. Kainoh, Y., Hiyori, T., andTamaki, Y. 1982. Kairomone of the egg-larval parasitoid,Ascogaster reticulatus Watanabe (Hymenoptera: Braconidae).Appl. Entomol. Zool. 17:102–110.Google Scholar
  11. Kawakami, T., andKainoh, Y. 1986. Host specificity of the egg-larval parasitoid,Ascogaster reticulatus Watanabe (Hymenoptera: Braconidae) among five tortricid and one noctuid species.Appl. Entomol. Zool. 21:8–14.Google Scholar
  12. Nettles, W.C., Jr., Morrison, R.K., Xie, Z.N., Ball, D., Shenkir, C.A., andVinson, S.B. 1982. Synergistic action of potassium chloride and magnesium sulfate on parasitoid wasp oviposition.Science 218:164–166.Google Scholar
  13. Nettles, W.C., Jr., Morrison, R.K., Xie, Z.N., Ball, D., Shenkir, C.A., andVinson, S.B. 1983. Effect of cations, anions and salt concentrations on oviposition byTrichogramma pretiosum in wax eggs.Entomol. Exp. Appl. 33:283–289.Google Scholar
  14. Thompson, S.N. 1986. Nutrition and in vitro culture of insect parasitoids.Anna. Rev. Entomol. 31:197–219.Google Scholar
  15. Vinson, S.B. 1977. Behavioral chemicals in the augmentation of natural enemies, pp. 237–279,in R.L. Ridgway and S.B. Vinson (eds.). Biological Control by Augmentation of Natural Enemies. Plenum Press, New York.Google Scholar
  16. Wu, Z.X. andQin, J. 1982. Oviposition response ofTrichogramma dendrolimi to the chemical contents of artificial eggs.Acta Entomol. Sin. 25:363–372.Google Scholar
  17. Xie, Z.N., Wu, V.Y., andDeng, X.L. 1982. Amino acid content inTrichogramma host eggs.Nat. Enemies Insects 4:22–25.Google Scholar
  18. Xie, Z.N., Nettles, W.C., Jr., Morrison, R.K., Irie, K., andVinson, S.B. 1986. Effect of ovipositional stimulants and diets on the growth and development ofTrichogramma pretiosum in vitro.Entomol. Exp. Appl. 42:119–124.Google Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Yooichi Kainoh
    • 1
  • Sadahiro Tatsuki
    • 2
  • Hajime Sugie
    • 1
  • Yoshio Tamaki
    • 1
  1. 1.Division of EntomologyNational Institute of Agro-Environmental SciencesIbarakiJapan
  2. 2.Institute of Agriculture and ForestryUniversity of TsukubaIbarakiJapan

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