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Use of ion-pairing reversed-phase liquid chromatography in separation of solanidine and solasodine

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Abstract

A method based on ion-pairing reversed-phase liquid chromatography was developed to separate and quantify the total alkaloid content inS. ptycanthum (eastern black nightshade). The method is shown to separate satisfactorily the aglycones and some of the glycoalkaloids. The mobile phase used was aqueous sodium dodecyl sulfate-acetonitrile.

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References

  • Bushway R.J., Barden, E.S., Bushway, A.W., andBushway, A.A. 1979. High-performance liquid Chromatographic separation of potato glycoalkaloids.J. Chromatogr. 178:533–541.

    Google Scholar 

  • Cham, B.E., andWilson, L. 1987. HPLC of glycoalkaloids fromSolanum sodomaeum.Planta Med. 53(1):59–62.

    Google Scholar 

  • Crabbe, P.G., andFryer, C. 1980. Rapid quantitative analysis of solasodine, solasodine glycosides and solasodiene by high-pressure liquid chromatography.J. Chromatogr. 187:87–100.

    Google Scholar 

  • Eldridge, A.C., andHockridge, M.E. 1983. High-performance liquid Chromatographic separation of eastern black nightshade (Solanum ptycanthum) glycoalkaloids.J. Agric. Food Chem. 31:1218–1220.

    Google Scholar 

  • Evenari, M. 1949. Germination inhibitors.Bot. Rev. 15:153–194.

    Google Scholar 

  • Hunter, I.R., andHeftmann, E. 1983. Isolation of glycoalkaloids with the chromatotron and their determination by high-performance liquid chromatography.J. Liquid Chromatogr. 6(2):281–289.

    Google Scholar 

  • Hunter, I.R., Walden, M.K., andHeftmann, E. 1980. High performance liquid chromatography ofSolanum andVeratrum alkaloids.J. Chromatogr. 198:363–366.

    Google Scholar 

  • Paquin, R., andLepage, M. 1963. Separation de la solanine, de la chaconine et de la solanidine par Chromatographie sur couches minces.J. Chromatogr. 12:57–62.

    Google Scholar 

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Ghazi, M., Matthees, D.P. Use of ion-pairing reversed-phase liquid chromatography in separation of solanidine and solasodine. J Chem Ecol 15, 2661–2666 (1989). https://doi.org/10.1007/BF01014724

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  • DOI: https://doi.org/10.1007/BF01014724

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