Abstract
Secretion from glands was observed when cercariae ofSchistosoma mansoni were exposed to certain lectins. Lectins fromMaclura pomifera, Pisum sativum, andTriticum vulgaris, were effective at 7.5 μ/ml. The effects of cercarial gland secretion caused byT. vulgaris agglutinin andArachis hypogaea agglutinin were blocked by pretreatment with the inhibiting glycan. Discharge of glands was not visualized after exposure of miracidia to lectins. The distribution of five labeled lectins was determined on live miracidia and cercariae. OnlyT. vulgaris agglutinin generally labeled the cercarial and miracidial bodies. Specific labeling occurred with the other lectins on the anterior, in glands or on their secretions, in flame cells in both stages, and on an unidentified ring of cells in miracidia. The possible mechanisms involved in changes caused by the lectins are discussed.
Similar content being viewed by others
References
Austin, F.G., Stirewalt, M.A., andDanziger, R.E. 1972.Schistosoma mansoni: Stimulatory effect of rat skin lipid fractions on cercarial penetration behavior.Exp. Parasitol. 31:217–224.
Bone, L.W., andBottjer, K.P. 1985. Cuticular carbohydrates of three nematode species and chemoreception byTrichostrongylus colubriformis.J. Parasitol. 71:235–238.
Dusenbery, D. 1980. Behavior of free living-nematodes, pp. 127–158,in B.M. Zuckerman (ed.). Nematodes as Biological Models, Vol. 1. Academic Press, New York.
Haas, W. 1984.Schistosoma mansoni: cercaricidal effect of 2-tetradecanoic acid, a penetration stimulant.Exp. Parasitol. 58:215–222.
Jansson, H.-B., andNordbring-Hertz, B. 1984. Involvement of sialic acid in nematode chemotaxis and infection by an endoparasitic nematophagous fungus.J. Gen. Microbiol. 130:39–43.
Jansson, H.-B., Jeyaprakash, A., Damon, R.A., andZuckerman, B.M. 1984.Caenorhabditis elegans andPanagrellus redivivus: Enzyme-mediated modification of chemotaxis.Exp. Parasitol. 58:270–277.
Jeyaprakash, A., Jansson, H.-B., Marban-Mendoza, N., andZuckerman, B.M. 1985.Caenorhabditis elegans: Lectin-mediated modification of chemotaxis.Exp. Parasitol. 59:90–97.
Kahane, I., andTully, J.G. 1976. Binding of plant lectins to mycoplasma cells and membranes.J. Bacteriol. 128:1–7.
Linder, E. 1985.Schistosoma mansoni: Visualization with fluorescent lectins of secretions and surface carbohydrates of living cercariae.Exp. Parasitol. 59:307–312.
Linder, E. 1986. Fluorochrome-labeled lectins reveal secreted glycoconjugates of schistosome larvae.Parasitol. Today 2:219–221.
Macinnis, A.J. 1965. Responses ofSchistosoma mansoni miracidia to chemical attractants.J. Parasitol. 51:731–746.
McClure, M., andZuckerman, B.M. 1982. Localization of cuticular binding sites of Concanavalin A onCaenorhabditis elegans andMeloidogyne incognita.J. Nematol. 14:39–44.
Monsigny, M., Kieda, C., andRoche, A.-C. 1983. Membrane glycoproteins, glycolipids and membrane lectins as recognition signals in normal and malignant cells.Biol. Cell. 47:95–110.
Murrell, K.D., Taylor, D.W., Vannier, W.E., andDean, D.A. 1978.Schistosoma mansoni: Analysis of surface membrane carbohydrates using lectins.Exp. Parasitol. 46:247–255.
Nordbring-Hertz, B., andMattiasson, B. 1979. Action of a nematode-trapping fungus shows lectin-mediated host-microorganism interaction.Nature 281:477–479.
Samuelson, J.C., andCaulfield, J.P. 1985. The cercarial glycocalyx ofSchistosoma mansoni.J. Cell. Biol. 100:1423–1434.
Schauer, R. 1982. Chemistry, metabolism and biological functions of sialic acids.Adv. Carbohyd, Chem. Biochem. 40:131–234.
Simpson, A.J.G., andMclaren, D.J. 1982.Schistosoma mansoni: Tegumental damage as a consequence of lectin binding.Exp. Parasitol. 53:105–116.
Spiegel, Y., Cohn, E., andSpiegel, S. 1982. Characterization of sialyl and galactosyl residues on the body wall of different plant parasitic nematodes.J. Nematol. 14:33–39.
Talmage, D.W., andClaman, H.N. 1967. External labeling of proteins with131I and125I,in A. William and M. Chase (eds.). Methods in Immunology and Immunochemistry, Vol. 1. Academic Press, New York.
Zuckerman, B.M., andJansson, H.-B. 1984. Nematode chemotaxis and possible mechanisms of host/prey recognition.Annu. Rev. Phytopathol. 22:95–113.
Zuckerman, B.M., Kahane, I., andHimmelhoch, S. 1979.Caenorhabditis briggsae andC. elegans: Partial characterization of cuticle surface carbohydrates.Exp. Parasitol. 47:419–424.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Coles, G.C., Jansson, H.B. & Zuckerman, B.M. Lectin studies of surface carbohydrates and induction of gland secretion in the free-living stages ofSchistosoma mansoni . J Chem Ecol 14, 691–700 (1988). https://doi.org/10.1007/BF01013916
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01013916