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A specific histochemical method for the demonstration of the activity of pancreatic lipase

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Synopsis

Emulsified long-chain triglyceride, a specific substrate for the enzyme pancreatic lipase (glycerol-ester hydrolase, EC 3.1.1.3), has been used in a modification of the Gomori technique for the demonstration of lipase. In the range of tissues examined (pancreas, testis, cardiac stomach and liver), true pancreatic lipase activity was revealed only in pancreatic tissue, by contrast with results obtained with less specific methods.

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Authors and Affiliations

  1. Department of Physiology, The London Hospital Medical College, University of London, E.I, London

    S. Jane Darnton & J. A. Barrowman

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  1. S. Jane Darnton
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  2. J. A. Barrowman
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Darnton, S.J., Barrowman, J.A. A specific histochemical method for the demonstration of the activity of pancreatic lipase. Histochem J 1, 551–557 (1969). https://doi.org/10.1007/BF01012861

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  • DOI: https://doi.org/10.1007/BF01012861

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