The Histochemical Journal

, Volume 6, Issue 4, pp 431–446 | Cite as

The histochemical demonstration of hydrolytic enzymes in adjuvant-induced arthritis in rats

  • A. D. Pearse
Papers

Summary

Acid phosphatase, alkaline phosphatase, leucine naphthylamidase and β-glucuronidase were studied histochemically in unfixed, undemineralized affected paws from rats with adjuvant-induced arthritis. Sections were prepared in a newly designed bone cryostat and it was found that untreated tissue sections gave the most satisfactory results. Acid and alkaline phosphatase activities were studied using azo dye techniques and the substrate naphthol AS-TR phosphate gave the best results with regard to sensitivity and localization.

Parallel to the pathological changes in the rats, alkaline and acid phosphatase activities increased with noticeable changes in localization in degraded zones. The degradation of the articular cartilage was accompanied by an extracellular particulate reaction for acid phosphatase around the chondrocytes. The increase in β-glucuronidase activity was confined to its usual localization in synovial and inflammatory cells. Leucine naphthylamidase activity increased together with a change of the localization in the connective tissue of the affected joints.

The results suggest that acid phosphatase, alkaline phosphatase and leucine naphthylamidase play an active role in cartilage and bone erosion in adjuvant arthritis, whereas β-glucuronidase remains localized in inflammatory and synovial cells and does not appear to participate actively in the destructive process.

Keywords

Alkaline Phosphatase Articular Cartilage Phosphatase Activity Acid Phosphatase Alkaline Phosphatase Activity 

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Copyright information

© Chapman and Hall Ltd 1974

Authors and Affiliations

  • A. D. Pearse
    • 1
  1. 1.Experimental Pathology DivisionThe Kennedy Institute of RheumatologyLondonUK

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