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Nucleoprotein staining. An analysis of a standardized trichloroacetic acid-Fast Green FCF procedure

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Synopsis

A standardized, highly reproducible modification of the original Alfert & Geschwind procedure (1953) has been formulated; it involves trichloroacetic acid extraction at 60°C and the use of a weak HCl-borate buffer for the dye solution.

A comparison of sections stained with Fast Green, Feulgen-Schiff, Toluidine Blue and Gallocyanin-chrome alum indicates that the trichloroacetic acid-Fast Green procedure does not reveal the actual amount of histone but instead simply indicates the excess of positive charges in the chromatin and cytoplasm remaining after the acid extraction.

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References

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Authors and Affiliations

  1. Department of Anatomy, University of Odense, Odense, Denmark

    P. Prentø

  2. Histochemical Laboratory, Department of Pathological Anatomy, Kommunehospitalet, Copenhagen, Denmark

    H. Lyon

Authors
  1. P. Prentø
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  2. H. Lyon
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Prentø, P., Lyon, H. Nucleoprotein staining. An analysis of a standardized trichloroacetic acid-Fast Green FCF procedure. Histochem J 5, 493–501 (1973). https://doi.org/10.1007/BF01012056

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  • DOI: https://doi.org/10.1007/BF01012056

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