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The effect of various fixation procedures on the digestability of sialomucins with neuraminidase

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The histochemical digestability with neuraminidase of sialomucin in mouse sublingual gland was studied in unfixed and formaldehyde vapour-fixed cryostat sections, and in sections prepared from paraffin-embedded material fixed in several alcohol- or formaldehyde-containing fixatives recommended for mucosubstances.

The removal of sialic acid residues from sections treated with neuraminidase was followed histochemically with the following staining methods: Azure A pH 3.5, Alcian Blue pH 2.5, Low Iron Diamine and Alcian Blue pH 2.5 followed by periodic acid-Schiff. When Goland's methanolic cyanuric chloride was used as fixative, only a partial loss of tissue basophilia was evident after enzyme incubation, but in tissues fixed in other ways a complete loss of histochemically detectable sialic acid residues was observed.

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Author notes

  1. Tapani E. Sorvari

    Present address: Department of Clinical Pathology, University Hospital, Kuopio, Finland

Authors and Affiliations

  1. Department of Pathological Anatomy, University of Turku, Finland

    Tapani E. Sorvari & Pekka A. Laurén

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  1. Tapani E. Sorvari
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  2. Pekka A. Laurén
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Sorvari, T.E., Laurén, P.A. The effect of various fixation procedures on the digestability of sialomucins with neuraminidase. Histochem J 5, 405–412 (1973). https://doi.org/10.1007/BF01011998

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  • DOI: https://doi.org/10.1007/BF01011998

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