Synopsis
The fixative properties of a number of mono- and dichlorotriazine dyestuffs were studied. These substances were, it was found, not pure and penetrated tissues poorly. The dichlorotriazines fixed and stained cells and their glycogen content moderately, well, but the monochloro derivatives produced poor fixation. Both groups of chlorotriazine derivatives reacted with proteins and glycogen but did not cross-link. In contrast, nucleic acids apparently did not react with these dyestuffs and were poorly stained.
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References
Bowes, J. H. &Cater, C. W. (1965). Crosslinking of collagen.J. Appl. Chem. 15, 296–344.
Dawson, T. L., Fern, A. S. &Preston, C. (1960). The reactions of cold-dyeing Procion dyes with cellulose.J. Soc. Dyers & Colourists 76, 210–17.
Ganote, C. E. &Moses, H. L. (1968). Light and dark cells as artifacts of liver fixation.Lab. Invest. 18, 740–5.
Giacobbe, E. &Amat, P. (1970). Contribute agli studi sui metodi di fissazione di preprarati citologici (Modifiche aj metodo di cianurizione).Boll. Soc. Ital. Biol. Sperim. 46, 691–4.
Goland, P. &Engel, M. (1963). Fixation of cells and tissues by chloro-s-triazines.J. Histochem. Cytochem. 11, 751–62.
Goland, P., Grand, N. G. &Katele, K. V. (1967). Cyanuric chloride and N-methyl morpholine in methanol as a fixative for polysaccharides.Stain Technol. 42, 41–51.
Goland, P., Grand, N. G., Green, F. J. &Booker, B. F. (1969). Immunofluorescence microscopy of cyanurated tissues.Stain Technol. 44, 227–33.
Horobin, R. W. &Murgatroyd, L. B. (1967). A comparison of rapid electrophoretic and chromatographic methods for the investigation of common histological dyes.Histochemie 11, 141–51.
Hess, R. &Pearse, A. G. E. (1959). Labelling of proteins with cellulose reactive dyes.Nature (London) 183, 260–1.
Hopwood, D. (1969a). Fixatives and fixation: a review.Histochem. F. 1, 323–60.
Hopwood, D. (1969b). A comparison of the crosslinking abilities of glutaraldehyde, formaldehyde and α-hydroxyadipaldehyde with bovine serum albumin and casein.Histochemie 17, 151–61.
Leach, A. A. (1965). The reaction of cyanuric chloride with gelatins and animal glues.J. Appl. Chem. 15, 78–84.
Maurer, H. R. (1971).Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis, 2nd Edn. Berlin: de Gruyter.
Mullen, T. C. (1961). Recent work on the applications of procion dyestuffs to leather.J. Soc. Leather Trades' Chemists 46, 162–75.
Pearse, A. G. E. (1968).Histochemistry: Theoretical and Applied. Vol. 1. 3rd Edn. London: Churchill.
Peracchia, C. &Mittler, B. S. (1972). New glutaraldehyde fixation procedures.J. Ultrastruct. Res. 39, 57–64.
Richards, E. G., Coll, J. A. &Gratzer, W. B. (1965). Disc electrophoresis of ribonucleic acids in polyacrylamide gels.Anal. Biochem. 12, 452–71.
St Groth, F. S. De, Webster, R. G. &Datyner, A. (1963). Two new staining procedures for quantitative estimation of protein on electrophoretic strips.Biochim. biophys. Acta 71, 377–91.
Seiton, E. C. &Encel, M. B. (1969). Reactive dyes as vital indicators of bone growth.Am. J. Anat. 126, 373–92.
Shore, J. (1968a). Mechanism of reaction of protein with reactive dyes. I-Literature survey.J. Soc. Dyers & Colourists 84, 408–12.
Shore, J. (1968b). Mechanism of reaction of proteins with reactive dyes. II-Reactivity of simple model compounds with chlorotriazine dyes.J. Soc. Dyers & Colourists 84, 413–22.
Shore, J. (1968c). Mechanism of the reaction of proteins with reactive dyes.J. Soc. Dyers & Colourists 84, 545–55.
Shore, J. (1969). Mechanism of reaction of proteins with reactive dyes. IV-Reactivity of wool with chlorotriazine dyes.J. Soc. Dyers & Colourists 85, 14–22.
Vickerstaff, T. (1957). Reactive dyes for textiles.J. Soc. Dyers & Colourists 73, 237–45.
Wegmann, J. (1960). Some relations between the chemical constitution of Cibacron dyes and their dyeing characteristics.J. Soc. Dyers & Colourists 76, 205–9.
Zacharius, R. M., Zell, T. E., Morrison, J. H. &Woodlock, J. J. (1969). Glycoprotein staining following electrophoresis on acrylamide gels.Anal. Biochem. 30, 148–52.
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Hopwood, D., Mellors, A.E. An investigation of the chlorotriazine derivatives as fixatives for tissues. Histochem J 5, 391–403 (1973). https://doi.org/10.1007/BF01011997
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DOI: https://doi.org/10.1007/BF01011997