Summary
The cellular and intracellular distribution of carbonic anhydrase isozyme II in rat cerebellum has been investigated with the electron microscope by the indirect antibody immunohistochemical technique. Unequivocal evidence is presented supporting the view that this enzyme is exclusively localized in oligodendrocytes. Myelin does not appear to contain detectable amounts of carbonic anhydrase though it is present in oligodendrocyte processes and in the layer of oligodendrocyte cytoplasm frequently seen to coat the external surface of myelinated fibres. The immune precipitate is found to be confined to the cytosol and the cytosolic surfaces of intracellular membranes. The data are discussed in relation to the possible function of the enzyme and the role of oligodendrocytes in the central nervous system.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdel-Akher, M., Hamilton, J. K., Montgomery, R. &Smith, F. (1952) A new procedure for the determination of the fine structure of polysaccharides.J. Am. Chem. Soc. 74, 4970–8.
Bourke, R. S. (1969) Studies of the development and subsequent reduction of swelling of mammalian cerebral cortex under isosomotic conditionsin vitro.Expl Brain Res. 8, 232–48.
Bourke, R. S., Gabelnick, H. L. &Young, O. M. (1970a) Mediated transport of chloride from blood into the cerebrospinal fluid.Expl Brain Res. 10, 17–38.
Bourke, R. S., Nelson, K. M., Naumann, R. A. &Young, O. M. (1970b) Studies on the production and subsequent reduction of swelling in primate cerebral cortex under isosmotic conditionsin vivo.Expl Brain Res. 10, 427–46.
Bourke, R. S. &Nelson, K. M. (1972) Further studies of the K+-dependent swelling of primate cerebral cortexin vivo: the enzymatic basis of the K+-dependent transport of chloride.J. Neurochem. 19, 663–85.
Cammer, W., Fredman, T., Rose, A. L. &Norton, W. T. (1976) Brain carbonic anhydrase: activity in isolated myelin and the effect of hexachlorophene.J. Neurochem. 27, 165–71.
Cammer, W., Bieler, L., Fredman, T. &Norton, W. T. (1977) Quantitation of myelin carbonic anhydrase: development and subfractionation of rat brain myelin and comparison with myelin from other species.Brain Res. 138, 17–28.
Fagg, G. E., Lane, J. D., Neuhoff, V. &Waenheldt, T. V. (1979) Carbonic anhydrase activity in myelin fractions from rat optic nerves.Neurosci. Lett. 12, 219–22.
Filippi, D., Sciaky, M., Limozin, N. &Laurent, G. (1978) Anhydrase carbonic du système nerveux central du rat. Isolement et propriétés.Biochimie 60, 99–102.
Chandour, M. S., Langley, O. K., Vincendon, G. &Gombos, G. (1979) Double labelling immunohistochemical technique provides evidence of the specificity of glial cell markers.J. Histochem. Cytochem. 27, 1634–7.
Ghandour, M. S., Langley, O. K., Vincendon, G., Gombos, G., Filippi, D., Limozin, N., Dalmasso, C. &Laurent, G. (1980) Immunochemical and immunohistochemical study of carbonic anhydrase II in adult rat cerebellum: a marker for oligodendrocytes.Neuroscience 5, 559–71.
Giacobini, E. (1961) Localization of carbonic anhydrase in the nervous system.Science, N.Y. 134, 1524–5.
Graham, R. C. &Karnovsky, M. J. (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.J. Histochem. Cytochem. 14, 291–302.
Holmes, R. S. (1977) Purification, molecular properties and ontogeny of carbonic anhydrase isozymes. Evidence for A, B and C isozymes in avian and mammalian tissues.Eur. J. Biochem. 78, 511–20.
Hsu, S. S. &Tashian, R. E. (1977) Genetic control of quantitative variation in carbonic anhydrase isoenzymes of mammals. I. The concentration within single erythrocytes of the mouse.Biochem. Genet. 15, 885–96.
Korhonen, L. K., Näätänen, E. &Hyyppä, M. (1964) A histochemical study of carbonic anhydrase in some parts of the mouse brain.Acta histochem. 18, 336–47.
Limozin, N., Filippi, D., Dalmasso, C. &Laurent, G. (1979) Dosage radio-immunologique des anhydrases carboniques I et II de rat. Application au système nerveux central au course de l'ontogénèse.Biochimie 61, 115–9.
Lindskog, S., Henderson, L. E., Kannan, K. K., Liljas, A., Nyman, P. O. &Strandberg, B. (1971) Carbonic anhydrase. InThe Enzymes (edited byBoyer, P. D.), Vol. 5, pp. 587–665. New York: Academic Press.
Palay, S. L. &Chan-Palay, V. (1974)Cerebellar Cortex Cytology and Organization. Berlin, Heidelberg, New York: Springer-Verlag.
Recister, A. M., Koester, M. K. &Noltmann, E. A. (1978) Discovery of carbonic anhydrase in rabbit skeletal muscle and evidence for its identity with basic muscle protein.J. biol. Chem. 253, 4143–52.
Reynolds, E. S. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.J. Cell Biol. 17, 208–12
Rose, S. P. R. (1967) Preparation of enriched fractions from cerebral cortex containing isolated, metabolically active neuronal and glial cells.Biochem. J. 102, 33–43.
Roussel, G., Delaunoy, J-P., Nussbaum, J-L. &Mandel, P. (1979) Demonstration of a specific localization of carbonic anhydrase C in the glial cells of rat CNS by an immunohistochemical method.Brain Res. 160, 47–55.
Schachner, M., Hedley-Whyte, E. T., Hsu, D. W., Schoonmaker, G. &Bignami, A. (1977) Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labelling.J. Cell Biol. 75, 67–73.
Sciaky, M., Filippi, D. & Laurent, G. (1975) On erythrocyte and brain carbonic anhydrase of the rat.10th Int. Congr. FEBS, Paris, Abstract, 778.
Sciaky, M., Limozin, N., Filippi-Foveau, D., Gulian, J-M. &Laurent-Tabusse, G. (1976) Structure primaire de l'anhydrase carbonique érythrocytaire bovine CI. II. Séquence complète.Biochimie 58, 1071–82.
Spicer, S. S., Stoward, P. J. &Tashian, R. E. (1979) The immunohistolocalization of carbonic anhydrase in rodent tissue.J. Histochem. Cytochem. 27, 820–31.
Tower, D. B. &Young, O. M. (1973) The activities of butyrylcholinesterase and carbonic anhydrase, the rate of anaerobic glycolysis, and the question of a constant density of glial cells in cerebral cortices of various mammalian species from mouse to whale.J. Neurochem. 20, 269–78.
Yandrasitz, J. R., Ernst, S. A. &Salganicoff, L. (1976) The subcellular distribution of carbonic anhydrase in homogenates of perfused rat brain.J. Neurochem. 27, 707–15.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Langley, O.K., Ghandour, M.S., Vincendon, G. et al. Carbonic anhydrase: An ultrastructural study in rat cerebellum. Histochem J 12, 473–483 (1980). https://doi.org/10.1007/BF01011962
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01011962