Summary
The peroxidase-antiperoxidase technique has been used to study sites of pituitary hormone storage and binding. Some recent findings from our laboratory show that the technique can make intriguing contributions to our understanding of pituitary cell function. In serial ulbra-thin sections, one can identify two or three hormones in a given cell. During pre-pubertal development, gonadotropes may contain adrenocorticotropin immunoreactivity. Brain releasing hormones may be stored or sequestered in granules of cells they stimulate. This report includes a discussion andcritique of our recent findings and interpretations which must be considered before one draws any conclusions about their biological significance.
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Childs, G. V., Cole D. E., Kubek, M., Tobin, R. B. &Wilber, J. F. (1978). Endogenous thyrotropin-releasing hormone in the anterior pituitary: Sites of activity as identified by immunocytochemical staining.J. Histochem. Cytochem. 26, 901–8.
Childs, G. V., Hon, C., Russell, L. R. &Gardner, P. J. (1978). Subcellular localization gonadotropins and testosterone in the developing foetal rat testis.J. Histochem. Cytochem. 26, 545–64.
Ellison, D. C. & Childs, G. V. (1980) An improved method for the rapid collection of serial cells for electron microscopic analysis of their immunocytochemical stain.J. Histochem. Cytochem. 28, (in press).
Hutson, J. C., Childs, G. V. &Gardner, P. J. (1979). Considerations for establishing the biological significance of immunocytochemical stains.J. Histochem. Cytochem. 27, 1201–2.
Hutson, J. C., Gardner, P. J. &Moriarty, G. C. (1977) Immunocytochemical localization of a follicle stimulating hormone-like molecule in the testis.J. Histochem. Cytochem. 25, 163–74.
Kawarai, Y. &Nakane, P. K. (1970). Localization of tissue antigen on the ultrathin sections with peroxidase labeled antibody method.J. Histochem. Cytochem. 18, 161–6.
Moriarty, G. C. (1977). Heterogeneity of ACTH-containing cells in the rat pituitary with emphasis on the structure and function of the intermediate lobe.Ann. N.Y. Acad. Sci. 297, 183–200.
Moriarty, G. C. &Halmi, N. S. (1972a) Electron microscopic localization of the adrenocorticotropin-producing cell with unlabeled antibody and soluble peroxidaseanti-peroxidase complex.J. Histochem. Cytochem. 20, 590–603.
Moriarty, G. C. &Halmi, N. S. (1972b) Adrenocorticotropin production by the intermediate lobe of the rat pituitary.Z. Zellforsch 132, 1–14.
Moriarty, G. C., Moriarty, C. M. &Sternberger, L. A. (1973) Ultrastructural immunocytochemistry with unlabeled antibodies and the peroxidase-anti-peroxidase complex: A technique more sensitive than radioimmunoassay.J. Histochem. Cytochem. 21, 825–33.
Moriarty, G. C. (1975). Electron microscopic-immunocytochemical studies of rat pituitary gonadotrophs: a sex difference in morphology and cytochemistry of LH cells.Endocrinology 97, 1215–25.
Moriarty, G. C. (1976) Immunocytochemistry of the pituitary glycoprotein hormones.J. Histochem. Cytochem. 24, 846–63.
Moriarty, G. C. &Garner, L. L. (1977) Immunocytochemical studies of cells in the rat adenohypophysis containing both ACTH and FSH.Nature. Lond. 265, 356–8.
Nakane, P. K. &Pierce, G. B., Jr. (1966). Enzyme-labeled antibodies: preparation and application for the localization of antigens.J. Histochem. Cytochem. 14, 929–31.
Nakane, P. K. &Pierce, G. B., Jr. (1967). Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens.J. Cell Biol. 33, 307–67.
Nakane, P. K. (1968). Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat.J. Histochem. Cytochem. 16, 557–68.
Nakane, P. K. (1970) Classifications of anterior pituitary cells with immunoenzyme histochemistry.J. Histochem. Cytochem. 18, 9–20.
Pelletier, G. (1979). Ultrastructural immunohistochemical localization of adrenocorticotropin and beta-lipotropin in the rat brain.J. Histochem. Cytochem. 27, 1046–8.
Phifer, R. F., Midgley, A. R. &Spicer, S. S. (1973). Immunohistologic and histologic evidence that follicle-stimulating and luteinizing hormones are present in the same cell types in the human pars distalis.J. clin. Endocrinol. 36, 125–41.
Sternberger, L. A., Hardy, P. H., Jr., Culculis, J. J. &Meyer, H. G. (1970). The unlabeled antibody method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti-peroxidase) and its use in identification of spirochetes.J. Histochem. Cytochem. 18, 315–33.
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Childs, G.V., Ellison, D.G. A critique of the contributions of immunoperoxidase cytochemistry to our understanding of pituitary cell function, as illustrated by our current studies of gonadotropes, corticotropes and endogenous pituitary GnRH and TRH. Histochem J 12, 405–418 (1980). https://doi.org/10.1007/BF01011957
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DOI: https://doi.org/10.1007/BF01011957