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Substrate protection during the fixation ofβ-glucuronidase: Cytochemical model system studies

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References

  • De Duve, C., Pressman, B. C., Gianetto, R., Wattiaux, R. &Appelmans, F. (1955) Tissue fractionation studies, 6. Intracellular distribution pattern of enzymes in rat liver tissue.Biochem. J. 60, 604–17.

    Google Scholar 

  • De Jong, A. S. H., Van Duijn, P. &Daems, W. Th. (1976) Cytochemical model system for microsomal rat liver glucose-6-phosphatase.J. Histochem. Cytochem. 24, 643–51.

    Google Scholar 

  • Doyle, M. L., Katzman, P. A. &Doisy, E. A. (1955) Production and properties of bacterial β-glucuronidase.J. biol. Chem. 217, 921–30.

    Google Scholar 

  • Fishman, W. H., Goldman, S. S. &De Lellis, R. (1967) Dual localization of β-glucuronidase on endoplasmic reticulum and in lysosomes.Nature, Lond. 213, 457–60.

    Google Scholar 

  • Janigan, D. T. (1964) Tissue enzyme fixation studies, 1. The effects of aldehyde fixation on β-glucuronidase, β-galactosidase,N-acetyl-β-glucosaminidase, and β-glucosidase in tissue blocks.Lab. Invest. 13, 1038–50.

    Google Scholar 

  • Meijer, A. E. F. H. &Israël, D. E. (1978a) Evaluation of histochemical observations of activity of acid hydrolases obtained with semipermeable membrane techniques: a combined histochemical and biochemical investigation. 1. The histochemical investigation.Histochemistry 57, 9–22.

    Google Scholar 

  • Meijer, A. E. F. H. &Israël, D. E. (1978b) Evaluation of histochemical observations of activity of acid hydrolases obtained with semipermeable membrane techniques: a combined histochemical and biochemical investigation. 2. The biochemical investigation and comparison with the histochemical observations.Histochemistry 57, 23–31.

    Google Scholar 

  • Papadimitriou, J. M. &Van Duijn, P. (1970) Effects of fixation and substrate protection on the isoenzymes of aspartate aminotransferase studied in a quantitative cytochemical model system.J. Cell Biol. 47, 71–83.

    Google Scholar 

  • Pearse, A. G. E. (1972)Histochemistry, Theoretical and Applied, 3rd edn, Vol. 2. pp. 808–818. Edinburgh, London: Churchill Livingstone.

    Google Scholar 

  • Raap, A. K. &Van Duijn, P. (1981) Enzyme loaded erythrocyte ghosts; a new microscopical model system for quantitative enzyme cytochemistry.J. Histochem. Cytochem. 29, 1418–24.

    Google Scholar 

  • Stahl, P. D. &Touster, O. (1971) β-Glucuronidase of rat liver lysosomes.J. biol. Chem. 246, 5398–406.

    Google Scholar 

  • VanDuijn, P. (1973) Fundamental aspects of enzyme cytochemistry. InElectron Microscopy and Cytochemistry, (edited byWisse, E., Daems, W. Th.,Molenaar, I. andVan Duijn, P.), pp. 3–33. Amsterdam: North Holland.

    Google Scholar 

  • Van Duijn, P. &Van Der Ploeg, M. (1980) Microscopic cytochemistry as matrix chemistry. InTrends in Enzyme Histochemistry and Cytochemistry, CIBA Foundation Symposium 73 (new series), pp. 209–299. Amsterdam, New York, Oxford: Excerpta Medica.

    Google Scholar 

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Authors and Affiliations

  1. Centro di Studio per l'Istochimica del C.N.R., Istituto di Anatomia Comparata, Università di Pavia, Piazza Botta 10, 27100, Pavia, Italy

    E. Prosperi

  2. Department of Histochemistry and Cytochemistry, University of Leiden, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands

    A. K. Raap

Authors
  1. E. Prosperi
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  2. A. K. Raap
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Prosperi, E., Raap, A.K. Substrate protection during the fixation ofβ-glucuronidase: Cytochemical model system studies. Histochem J 14, 689–692 (1982). https://doi.org/10.1007/BF01011901

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  • DOI: https://doi.org/10.1007/BF01011901

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