The Histochemical Journal

, Volume 14, Issue 3, pp 409–413 | Cite as

A simple and rapid method for osteoclast identification using a histochemical method for acid phosphatase

  • Yehya A. Mostafa
  • Ralph A. MeyerJr
  • Rocco Latorraca
Papers

Summary

A detailed description of a simple technique for acid phosphatase demonstration in osteoclast cells is presented. The method is short, relatively uninvolved and allows the use of undecalcified bone sections. All materials and chemicals used are commercially available. The sections were stained histochemically using naphthol AS-B1 phosphoric acid as a substrate and counter stained with Mayer's Haematoxylin. Sections prepared by this method showed good results and enable accurate measurement of osteoclast counts.

Keywords

Phosphoric Acid Acid Phosphatase Rapid Method Haematoxylin Naphthol 

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References

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Copyright information

© Chapman and Hall Ltd 1982

Authors and Affiliations

  • Yehya A. Mostafa
    • 1
  • Ralph A. MeyerJr
    • 1
  • Rocco Latorraca
    • 2
  1. 1.Department of Basic Sciences, School of DentistryMarquette UniversityMilwaukeeUSA
  2. 2.Trinity Memorial HospitalCudahyUSA

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