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Alkaline phosphatase histochemistry and early renal cortical damage

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Synopsis

Alkaline phosphatase was studied in the proximal tubules of kidneys from rats treated with various nephrotoxins. Changes in activity and location of alkaline phosphatase were demonstrated both with dose levels which produced serious morphological lesions and with dose levels which caused no morphological changes. By varying the duration of treatment it was shown that changes in alkaline phosphatase activity occurred before morphological changes appeared. Electron microscopic studies showed a close relationship between changes in alkaline phosphatase activity and damage of the brush border. It is concluded that alkaline phosphatase histochemistry could possibly be used as an early and sensitive index of nephrotoxicity.

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Author notes

  1. N. Gyrd-Hansen (Wellcome-Carlsberg Travelling Research Fellow 1972/73)

    Present address: Department of Pharmacology and Toxicology, Royal Veterinary and Agricultural University, Copenhagen, Denmark

Authors and Affiliations

  1. The British Industrial Biological Research Association, Carshalton, Surrey

    N. Gyrd-Hansen (Wellcome-Carlsberg Travelling Research Fellow 1972/73)

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  1. N. Gyrd-Hansen
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Gyrd-Hansen, N. Alkaline phosphatase histochemistry and early renal cortical damage. Histochem J 6, 199–209 (1974). https://doi.org/10.1007/BF01011807

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  • DOI: https://doi.org/10.1007/BF01011807

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