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Comparison of the effects of two sources of hyaluronidase on tissue sections using visual estimates and microdensitometric measurements

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Synopsis

Comparison of two hyaluronidase preparations showed no difference to exist between them in their action on various tissue components. The enzyme can be stored in buffer for 3 weeks at 5°C without loss of potency. There was a good correlation between visual estimates of staining and microdensitometric measurements.

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References

  • Leppi, T. J. &Stoward, P. J. (1965) On the use of testicular hyaluronidase for identification of acid mucopolysaccharides in tissue sections.J. Histochem. Cytochem. 13, 406–7.

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  • Margolis, R. V. &Margolis, R. K. (1970). Ribonuclease and desoxyribonuclease activity in hyaluronidase preparations.Analyt. Biochem. 35, 77–82.

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Authors and Affiliations

  1. Department of Veterinary Anatomy, University of Bristol, UK

    W. F. Butler & I. Pousty

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  1. W. F. Butler
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  2. I. Pousty
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Butler, W.F., Pousty, I. Comparison of the effects of two sources of hyaluronidase on tissue sections using visual estimates and microdensitometric measurements. Histochem J 6, 117–120 (1974). https://doi.org/10.1007/BF01011800

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  • DOI: https://doi.org/10.1007/BF01011800

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