Synopsis
Lower labial, buccal and sublingual glands have been examined. The lower labial glands contained a greater proportion of seromucous cells than the buccal and sublingual glands. In the latter glands there was a greater proportion of mucous cells that are stainable with the Alcian Blue critical electrolyte concentration technique with 0.2m MgCl2 and above than in the lower labial glands. Granules of acid phosphatase, β-glucuronidase and E600-resistant esterase reaction product, presumbly, representing lysosomal enzyme activity, were usually more prominent in seromucous than in mucous cells. Thiamine pyrophosphatase and inosine diphosphatase activities were demonstrated in a Golgi-like distribution, which was more prominent in mucous cells. Diffuse non-specific esterase, NADH-and NADPH- diaphorases and peroxidase activities were more prominent in seromucous cells. However, diffuse acid phosphatase activity was more prominent in mucous cells. Granules of β-glucuronidase, acid phosphatase and E600-resistant esterase reaction product were seen in ductal cells, and were often concentrated in the supranuclear region. Arylamidase activity was present lining the lumen of some ducts and acini.
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References
Baradi, A. F. &Booher, D. W. (1968). The effect of protein synthesis inhibition on the adenosine-triphosphatase histochemical reaction in the parotid gland acini of rabbit and rat.Archs. oral Biol. 13, 803–8.
Barka, T. &Anderson, P. J. (1962). Histochemical methods for acid phosphatase using hexazonium pararosanilin as coupler.J. Histochem. Cytochem. 10, 741–53.
Bulmer, D. &Fisher, A. W. F. (1970). Studies on the characterization, and localization of rat placental esterases.J. Histochem. Cytochem. 18, 722–9.
Carlsöö, B. (1971). Histochemical observations on endogenous lactoperoxidase activity of the bovine submandibular salivary gland.Histochem. J. 3, 185–92.
Carlsöö, B., Kumlien, A. &Bloom, G. D.. (1971). A comparative histochemical study of peroxidase activity in the submandibular glands of five mammalian species including man.Histochemie 26, 80–8.
Chisholm, D. M., Stephen, K. W., Robertson, J. W. K. &Harden, R. MCG. (1971). Iodide concentration by human labial salivary glands.Archs oral Biol. 16, 1245–9.
Davis, B. J. (1959). Histochemical demonstration of erythrocyte esterases.Proc. Soc. exp. Biol. Med. 101, 90–3.
Emmelin, N. (1967). Nervous control of salivary glands.Handbook of Physiology, Section 6: Alimentary Canal, Volume II: Secretion, pp. 595–632. Washington: American Physiological Society.
Eversole, L. R. (1972). The histochemistry of mucosubstances in human minor salivary glands.Archs oral Biol. 17, 1225–39.
Garrett, J. R. &Harrison, J. D. (1970). Alkaline-phosphatase and adenosine-triphosphatase histochemical reactions in the salivary glands of cat, dog and man, with particular reference to the myoepithelial cells.Histochemie 24, 214–29.
Graham, R. C. &Karnovsky, M. J. (1970). The early stages of absorption of injected horse-radish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.J. Histochem. Cytochem. 14, 291–302.
Hand, A. R. (1971). Morphology and cytochemistry of the Golgi apparatus of rat salivary gland acinar cells.Am. J. Anat. 130, 141–58.
Harrison, J. D. (1972). The effects of duct ligation of salivary glands of cat studied by histological and histochemical methods.Ph.D. Thesis, University of London.
Hayashi, M., Nakajima, Y. &Fishman, W. H. (1964). The cytologic demonstration of β-glucuronidase employing naphthol AS-BI glucuronide and hexazonium pararosanilin; a preliminary report.J. Histochem. Cytochem. 12, 293–7.
Junqueira, L. C. U. (1964). Studies on the physiology of rat and mouse submaxillary glands. III. On the function of the striated ducts of the mammalian salivary glands. In:Salivary Glands and their Secretions (eds. L. M. Screebny & J. Meyer), pp. 123–8. London: Pergamon Press.
Koblle, G. B. (1955). The histochemical identification of acetylcholinesterase in cholinergic, adrenergic and sensory neurons.J. Pharmac. exp. Ther. 114, 167–84.
Kumlien, A. (1972). Iodine metabolism and peroxidase activity in salivary glands.Acta endocr. Copenh. 70, 239–46.
Mowry, R. W. (1956). Alcian blue technics for the histochemical study of acidic carbohdrates.J. Histochem. Cytochem. 4, 407.
Nachlas, M. M., Monis, B., Rosenblatt, D., &Seligman, A. M. (1960). Improvement in the histochemical localization of leucine aminopeptidase with a new substrate,l-leucyl-4-methoxy-2-naphthylamide.J. biophys. biochem. Cytol. 7, 261–4.
Nachlas, M. M., Tsou, K. C., De Souza, E., Cheng, C. S. &Seligman, A. M. (1957). Cytochemical demonstration of succinic dehydrogenase by the use of a newp-nitrophenyl substituted detetrazole.J. Histochem. Cytochem. 5, 420–36.
Novikoff, A. B. (1962). Cytochemical staining methods for enzyme activities: their application to the rat parotid gland.Jewish mem. Hosp. Bull. 7, 70–93.
Novikoff, A. B. (1970). Their phosphatase controversy: love's labours lost.J. Histochem. Cytochem. 18 916–17.
Patterson, E. K., Hsiao, S. H. &Keppel, A. (1963). Studies on dipeptidases and aminopeptidases. I. Distinction between leucine aminopeptidase and enzymes that hydrolyzel-leucyl-β-naphthylamide.J. biol. Chem. 238, 3611–20.
Rosenthal, A. S., Moses, H. L. &Ganote, C. E. (1970). Interpretation of phosphatase cytochemical data.J. Histochem. Cytochem. 18, 915.
Scarpelli, D. G., Hess, R., &Pearse, A. G. E. (1958). The cytochemical localization of oxidative enzymes.i. Diphosphopyridine nucleotide diaphorase and triphosphopyridine nucleotide diaphorase.J. biophys. biochem. Cytol. 4, 747–52.
Scott, J. E. &Dorling, J. (1965). Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian Blue in salt solutions.Histochemie 5, 221–33.
Smith, R. E. &Farquahar, M. G. (1970). Modulation in nucleoside diphosphatase activity of mammotrophic cells of the rat adenohypophysis during secretion.J. Histochem. Cytochem. 18, 237–50.
Spicer, S. S., Horn, R. G. &Leppi, T. G. (1967). Histochemistry of connective tissue mucopolysaccharides. In:The Connective Tissue (eds. B. M. Wagner & D. E. Smith), pp. 251–303. Baltimore: Williams & Wilkins.
Stoward, P. J. (1967). The histochemical properties of some periodate-reactive mucosubstances of the pregnant Syrian hamster before and after methylation with methanolic thionyl chloride.Jl R Microsc. Soc. 87, 77–103.
Stutte, H. J. (1967). Hexazotiertes Triamino-tritolyl-methanchlorid (Neufuchsin) als Kupplungssalz in der Fermenthistochemie.Histochemie 8, 327–31.
Tandler, B., Denning, C. R., Mandel, I. D. &Kutscher, A. H. (1969). Ultrastructure of human labial salivary glands. I. Acinar secretory cells.J. Morph. 127, 383–408.
Tandler, B., Denning, C. R., Mandel, I. D., &Kutscher, A. H. (1970). Ultrastructure of human labial salivary glands. III. Myoepithelium and ducts.J. Morph. 130, 227–46.
Wachstein, M. &Meisel, E. (1957). Histochemistry of hepatic phosphatases at a physiologic pH.Am. J. clin. Path. 27, 13–23.
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Harrison, J.D. Minor salivary glands of man: Enzyme and mucosubstance histochemical studies. Histochem J 6, 633–647 (1974). https://doi.org/10.1007/BF01011504
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DOI: https://doi.org/10.1007/BF01011504