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The Histochemical Journal

, Volume 6, Issue 6, pp 633–647 | Cite as

Minor salivary glands of man: Enzyme and mucosubstance histochemical studies

  • J. D. Harrison
Papers

Synopsis

Lower labial, buccal and sublingual glands have been examined. The lower labial glands contained a greater proportion of seromucous cells than the buccal and sublingual glands. In the latter glands there was a greater proportion of mucous cells that are stainable with the Alcian Blue critical electrolyte concentration technique with 0.2m MgCl2 and above than in the lower labial glands. Granules of acid phosphatase, β-glucuronidase and E600-resistant esterase reaction product, presumbly, representing lysosomal enzyme activity, were usually more prominent in seromucous than in mucous cells. Thiamine pyrophosphatase and inosine diphosphatase activities were demonstrated in a Golgi-like distribution, which was more prominent in mucous cells. Diffuse non-specific esterase, NADH-and NADPH- diaphorases and peroxidase activities were more prominent in seromucous cells. However, diffuse acid phosphatase activity was more prominent in mucous cells. Granules of β-glucuronidase, acid phosphatase and E600-resistant esterase reaction product were seen in ductal cells, and were often concentrated in the supranuclear region. Arylamidase activity was present lining the lumen of some ducts and acini.

Keywords

Thiamine Acid Phosphatase Inosine Ductal Cell Alcian Blue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baradi, A. F. &Booher, D. W. (1968). The effect of protein synthesis inhibition on the adenosine-triphosphatase histochemical reaction in the parotid gland acini of rabbit and rat.Archs. oral Biol. 13, 803–8.Google Scholar
  2. Barka, T. &Anderson, P. J. (1962). Histochemical methods for acid phosphatase using hexazonium pararosanilin as coupler.J. Histochem. Cytochem. 10, 741–53.Google Scholar
  3. Bulmer, D. &Fisher, A. W. F. (1970). Studies on the characterization, and localization of rat placental esterases.J. Histochem. Cytochem. 18, 722–9.Google Scholar
  4. Carlsöö, B. (1971). Histochemical observations on endogenous lactoperoxidase activity of the bovine submandibular salivary gland.Histochem. J. 3, 185–92.Google Scholar
  5. Carlsöö, B., Kumlien, A. &Bloom, G. D.. (1971). A comparative histochemical study of peroxidase activity in the submandibular glands of five mammalian species including man.Histochemie 26, 80–8.Google Scholar
  6. Chisholm, D. M., Stephen, K. W., Robertson, J. W. K. &Harden, R. MCG. (1971). Iodide concentration by human labial salivary glands.Archs oral Biol. 16, 1245–9.Google Scholar
  7. Davis, B. J. (1959). Histochemical demonstration of erythrocyte esterases.Proc. Soc. exp. Biol. Med. 101, 90–3.Google Scholar
  8. Emmelin, N. (1967). Nervous control of salivary glands.Handbook of Physiology, Section 6: Alimentary Canal, Volume II: Secretion, pp. 595–632. Washington: American Physiological Society.Google Scholar
  9. Eversole, L. R. (1972). The histochemistry of mucosubstances in human minor salivary glands.Archs oral Biol. 17, 1225–39.Google Scholar
  10. Garrett, J. R. &Harrison, J. D. (1970). Alkaline-phosphatase and adenosine-triphosphatase histochemical reactions in the salivary glands of cat, dog and man, with particular reference to the myoepithelial cells.Histochemie 24, 214–29.Google Scholar
  11. Graham, R. C. &Karnovsky, M. J. (1970). The early stages of absorption of injected horse-radish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.J. Histochem. Cytochem. 14, 291–302.Google Scholar
  12. Hand, A. R. (1971). Morphology and cytochemistry of the Golgi apparatus of rat salivary gland acinar cells.Am. J. Anat. 130, 141–58.Google Scholar
  13. Harrison, J. D. (1972). The effects of duct ligation of salivary glands of cat studied by histological and histochemical methods.Ph.D. Thesis, University of London.Google Scholar
  14. Hayashi, M., Nakajima, Y. &Fishman, W. H. (1964). The cytologic demonstration of β-glucuronidase employing naphthol AS-BI glucuronide and hexazonium pararosanilin; a preliminary report.J. Histochem. Cytochem. 12, 293–7.Google Scholar
  15. Junqueira, L. C. U. (1964). Studies on the physiology of rat and mouse submaxillary glands. III. On the function of the striated ducts of the mammalian salivary glands. In:Salivary Glands and their Secretions (eds. L. M. Screebny & J. Meyer), pp. 123–8. London: Pergamon Press.Google Scholar
  16. Koblle, G. B. (1955). The histochemical identification of acetylcholinesterase in cholinergic, adrenergic and sensory neurons.J. Pharmac. exp. Ther. 114, 167–84.Google Scholar
  17. Kumlien, A. (1972). Iodine metabolism and peroxidase activity in salivary glands.Acta endocr. Copenh. 70, 239–46.Google Scholar
  18. Mowry, R. W. (1956). Alcian blue technics for the histochemical study of acidic carbohdrates.J. Histochem. Cytochem. 4, 407.Google Scholar
  19. Nachlas, M. M., Monis, B., Rosenblatt, D., &Seligman, A. M. (1960). Improvement in the histochemical localization of leucine aminopeptidase with a new substrate,l-leucyl-4-methoxy-2-naphthylamide.J. biophys. biochem. Cytol. 7, 261–4.Google Scholar
  20. Nachlas, M. M., Tsou, K. C., De Souza, E., Cheng, C. S. &Seligman, A. M. (1957). Cytochemical demonstration of succinic dehydrogenase by the use of a newp-nitrophenyl substituted detetrazole.J. Histochem. Cytochem. 5, 420–36.Google Scholar
  21. Novikoff, A. B. (1962). Cytochemical staining methods for enzyme activities: their application to the rat parotid gland.Jewish mem. Hosp. Bull. 7, 70–93.Google Scholar
  22. Novikoff, A. B. (1970). Their phosphatase controversy: love's labours lost.J. Histochem. Cytochem. 18 916–17.Google Scholar
  23. Patterson, E. K., Hsiao, S. H. &Keppel, A. (1963). Studies on dipeptidases and aminopeptidases. I. Distinction between leucine aminopeptidase and enzymes that hydrolyzel-leucyl-β-naphthylamide.J. biol. Chem. 238, 3611–20.Google Scholar
  24. Rosenthal, A. S., Moses, H. L. &Ganote, C. E. (1970). Interpretation of phosphatase cytochemical data.J. Histochem. Cytochem. 18, 915.Google Scholar
  25. Scarpelli, D. G., Hess, R., &Pearse, A. G. E. (1958). The cytochemical localization of oxidative enzymes.i. Diphosphopyridine nucleotide diaphorase and triphosphopyridine nucleotide diaphorase.J. biophys. biochem. Cytol. 4, 747–52.Google Scholar
  26. Scott, J. E. &Dorling, J. (1965). Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian Blue in salt solutions.Histochemie 5, 221–33.Google Scholar
  27. Smith, R. E. &Farquahar, M. G. (1970). Modulation in nucleoside diphosphatase activity of mammotrophic cells of the rat adenohypophysis during secretion.J. Histochem. Cytochem. 18, 237–50.Google Scholar
  28. Spicer, S. S., Horn, R. G. &Leppi, T. G. (1967). Histochemistry of connective tissue mucopolysaccharides. In:The Connective Tissue (eds. B. M. Wagner & D. E. Smith), pp. 251–303. Baltimore: Williams & Wilkins.Google Scholar
  29. Stoward, P. J. (1967). The histochemical properties of some periodate-reactive mucosubstances of the pregnant Syrian hamster before and after methylation with methanolic thionyl chloride.Jl R Microsc. Soc. 87, 77–103.Google Scholar
  30. Stutte, H. J. (1967). Hexazotiertes Triamino-tritolyl-methanchlorid (Neufuchsin) als Kupplungssalz in der Fermenthistochemie.Histochemie 8, 327–31.Google Scholar
  31. Tandler, B., Denning, C. R., Mandel, I. D. &Kutscher, A. H. (1969). Ultrastructure of human labial salivary glands. I. Acinar secretory cells.J. Morph. 127, 383–408.Google Scholar
  32. Tandler, B., Denning, C. R., Mandel, I. D., &Kutscher, A. H. (1970). Ultrastructure of human labial salivary glands. III. Myoepithelium and ducts.J. Morph. 130, 227–46.Google Scholar
  33. Wachstein, M. &Meisel, E. (1957). Histochemistry of hepatic phosphatases at a physiologic pH.Am. J. clin. Path. 27, 13–23.Google Scholar

Copyright information

© Chapman and Hall Ltd 1974

Authors and Affiliations

  • J. D. Harrison
    • 1
  1. 1.Department of Oral PathologyKing's College Hospital Dental SchoolLondonUK

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