Skip to main content
SpringerLink
Log in

Erythrocyte agglomeration and survival studies in citrate-phosphate-dextrose (CPD) units

Agglomeration und Überlebensrate der Erythrozyten in CPD-Konserven

  • Original Works
  • Published:
Blut Aims and scope Submit manuscript

Zusammenfassung

In einer früheren Arbeit wurde über eine vermehrte Agglomerationsneigung von in CPD aufbewahrten Erythrozyten berichtet. In der jetzt vorliegenden Arbeit wurden als Ausdruck des Alterungsprozesses von Blutkonserven folgende Werte geprüft: Agglomeration der Erythrozyten; freies Hämoglobin; ATP; osmotische Resistenz und bestimmte Enzymaktivitäten. Anschließend wurden die gelagerten Erythrozyten mit51Cr markiert, und ihre 24-Stunden-Überlebensrate wurde durch Autotransfusion bestimmt. Der Agglomerationstest war bereits positiv, wenn die Überlebensrate noch 70 % betrug. Frische Blutkonserven waren durch hohe ATP-Spiegel und negative Agglomeration, alte Konserven durch gegenteilige Befunde gekennzeichnet. Bei mittlerer Aufbewahrungsdauer waren ATP-Spiegel und Agglomeration nicht zuverlässig korreliert. Der Agglomerationstest scheint den Alterungsgrad von Erythrozytenkonserven zuverlässig anzuzeigen. Jedoch scheinen noch weitere Untersuchungen nötig, bevor entschieden werden kann, ob dieser Test auch zuverlässig mit der Lebensfähigkeit der Erythrozyten nach Transfusion korreliert ist.

Summary

We have previously reported increased tendency to agglomeration in CPD-stored erythrocytes. In the present study we investigated the deterioration pattern of fresh units, as detected by agglomeration, free hemoglobin levels, ATP levels, osmotic fragility, and certain enzymatic activities. When the negative test was converted to a positive one, the 24-h survival values of these units were determined by autotransfusion. A positive agglomeration occurred while viability was still above 70%. Fresh units had high ATP levels and negative agglomeration while old units had low ATP levels and positive agglomeration. However, in intermediate-storage units, no definite correlation between ATP and agglomeration was found.

Agglomeration seems to reflect the degree of changes in stored erythrocytes. However, further studies are needed before this test may serve for prediction of post-transfusion viability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Beutler, E.: Experimental blood preservatives for liquid storage. In: The human red cell in vitro, Greenwalt, T. J., Jamieson, G. A. (eds.). New York: Grune and Stratton 1974

    Google Scholar 

  2. Beutler, E.: Red cell metabolism: A manual of biochemical methods. 2nd edition. New York: Grune and Stratton 1975

    Google Scholar 

  3. Dern, R. J., Gwinn, R. P., Wiorkowski, J. J.: Studies on the preservation of human blood. I. Variability in erythrocytes storage characteristics among healthy donors. J. Lab. Clin. Med.67, 955–965 (1966)

    Google Scholar 

  4. De Venuto, F., Wilson, S. M.: Steroid hormones in the preservation of human blood. Transfusion12, 53–58 (1972)

    Google Scholar 

  5. Feo, C., Mohandas, N.: Clarification of role of ATP in red cell morphology and function. Nature (London)265, 166–268 (1977)

    Google Scholar 

  6. Huggins, C. E.: Reversible agglomeration used to remove dimethylsulfoxide from large volume of frozen blood. Science139, 504–505 (1963)

    Google Scholar 

  7. Huggins, C.E.: Frozen Blood: theory and practice. JAMA193, 941–944 (1965).

    Google Scholar 

  8. Meyerstein, N., Mazor, D., Dvilansky, A.: Changes in agglomeration of human red blood cells in liquid storage in CPD media, Transfusion17, 115–122 (1977)

    Google Scholar 

  9. Mollison, P.L.: Blood transfusion in clinical medicine. 5th Edition. Oxford: Blackwell 1972

    Google Scholar 

  10. Pollack, W., Reckel, R. P.: The zeta potential and hemagglutination with Rh antibodies. Int. Arch. Allergy Appl. Immunol.38, 482–496 (1970)

    Google Scholar 

  11. Schrier, S. L., Krueger, J., Zunga, I., Hardy, B.: Red blood cell membrane storage lesion. Blood50 [Suppl. 1], 310, 1977

    Google Scholar 

  12. Shields, C. E., Kaplan, H. S., Dawson, R. B.: Biological alterations occurring during red cell preservation. In: Red cell metabolism and function. Brewer, G. J. (ed.). New York: Plenum Press 1970

    Google Scholar 

  13. Valeri, C. R.: Liquid and freeze preservation of red blood cells. In: The red blood cell. Surgenor, S. M. (ed.). New York: Academic Press 1974

    Google Scholar 

  14. Valeri, C. R.: Blood products and substitutes: benefits and risks. In: Blood banking and the use of frozen blood products. Cleveland: CRC Press 1976

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

    N. Meyerstein

  2. Research and Development Authority, Ben-Gurion University of the Negev, Beer-Sheva, Israel

    D. Mazor

  3. Soroka University Hospital and Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

    A. Dvilansky

Authors
  1. N. Meyerstein
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Mazor
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Dvilansky
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Meyerstein, N., Mazor, D. & Dvilansky, A. Erythrocyte agglomeration and survival studies in citrate-phosphate-dextrose (CPD) units. Blut 39, 211–216 (1979). https://doi.org/10.1007/BF01008450

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01008450

Schlüsselwörter

Key words

Search

Navigation