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Acid alpha-naphthyl acetate esterase in hairy cell leukemia cells and other cells of the hematopoietic system

Saure α-Naphthyl-Acetat-Esterase in den Zellen von Haarzell-Leukämien und anderen Zellen des hämatopoetischen Systems

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Zusammenfassung

Das Vorkommen und das Reaktionsbild vonα-Naphthyl-Acetat-Esterase bei pH 5,8 (saure Esterase) wurde in den Zellen von 10 Haarzell-Leukämien untersucht. Alle 10 Haarzell-Leukämie-Fälle — darunter zwei Fälle mit nur vereinzelten tartratresistenten saure Phosphatase-positiven Zellen — waren mäßig stark bis stark saure Esterase-positiv. Die saure Esterase-Aktivität war in feinen bis groben Granula meist semizirkulär um den Zellkern verteilt. Dieses Reaktionsbild fand sich weder in normalen noch in neoplastischen T-Zellen, B-Zellen, myeloischen Zellen oder histiozytischen Zellen. Die Zellen einiger B-Zell-Lymphome und von Plasmozytomen des Knochenmarkes zeigten ein relativ gleichmäßiges feinoder mittelgrobgranuläres saure Esterase-Muster, das nur bei einzelnen Plasmozytomen eine gewisse Ähnlichkeit mit dem saure Esterase-Muster von Haarzellen aufwies. Insgesamt scheint eine fein bis grobgranuläre saure Esterase-Reaktivität ein charakteristisches Merkmal für Haarzellen darzustellen und damit von diagnostischer Bedeutung für die Haarzell-Leukämie zu sein.

Summary

The activity ofα-naphthyl acetate esterase at an acid pH (ANAE) was investigated in 10 cases of hairy cell leukemia. All 10 cases, including two cases with only a few tartrate-resistant acid phosphatase-reactive cells, revealed a moderate or strong ANAE reaction. There was a characteristic pattern of activity consisting of small, medium-sized, or large distinct granules often distributed in a semicircle in the cytoplasm, but sparing the nucleus of hairy cells. This reaction pattern was not found in T cells, T cell-derived leukemias, normal B cells, a number of B-cell lymphomas, myeloid cells, myeloid leukemias (including monocyte-derived leukemias), reticulum cell sarcoma, or malignant reticulosis. The cells from some B-cell lymphomas and plasmacytoma showed a relatively homogeneous pattern of fine or moderately coarse ANAE-positive granules that was similar to that of hairy cells only in some cases of plasmacytoma. Thus, fine to coarse granular ANAE reactivity is characteristic of hairy cells and is of potential diagnostic value for hairy cell leukemia.

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References

  1. Bøyum, A.: Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Lab. Invest.21 [Suppl. 97], 77–89 (1968)

    Google Scholar 

  2. Braylan, R.C., Jaffe, E. S., Berard, C. W.: Surface characteristics of Hodgkin's lymphoma cells. [Letter to the Editor] Lancet 1974II, 1328–1329

    Google Scholar 

  3. Broder, S., Edelson, R. L., Lutzner, M. A., Nelson, D. L., MacDermott, R. P., Durm, M. E., Goldman, C. K., Meade, B. D., Waldmann, T. A.: The Sézary syndrome. A malignant proliferation of helper T cells. J. Clin. Invest.58, 1297–1306 (1976)

    Google Scholar 

  4. Burke, J. S., Byrne, G.E., Rappaport, H.: Hairy cell leukemia (leukemic reticuloendotheliosis). I. A clinical pathologic study of 21 patients. Cancer33, 1399–1410 (1974)

    Google Scholar 

  5. Buskard, N. A., Catovsky, D., Okos, A., Goldman, J. M., Galton, D. A. G.: Prolymphocytic leukaemia: Cell studies and treatment by leucapheresis. In: Maligne Lymphome und monoklonale Gammopathien. Hämatologie und Bluttransfusion, Bd. 18. Löffler, H. (ed.), pp. 237 bis 253. München: Lehmanns 1976

    Google Scholar 

  6. Catovsky, D., Papamichail, M., Okos, A., Miliani, E., Holborow, E. J.: Formation of mouse red cell rosettes by “hairy” cells. Biomedicine23, 81–84 (1975)

    Google Scholar 

  7. Catovsky, D., Pettit, J. E., Galton, D. A. G., Spiers, A. S. D., Harrison, C. V.: Leukaemic reticuloendotheliosis (“hairy” cell leukaemia): A distinct clinico-pathological entity. Br. J. Haematol.26, 9–27 (1974)

    Google Scholar 

  8. Düllmann, J., Wulfhekel, U., Drescher, S., Hausmann, K.: Die Haarzellenhämoblastose (“hairy cell leukemia”). Klinische, licht- und elektronenmikroskopische Befunde. Dtsch. Med. Wochenschr.99, 859–863 (1974)

    Google Scholar 

  9. Flandrin, G., Daniel, M. T., Fourcade, M., Chelloul, N.: Leucémie à “tricholeucocyte” (hairy cell leukemia), étude clinique et cytologique de 55 observations. Nouv. Rev. Fr. Hematol.13, 609–640 (1973)

    Google Scholar 

  10. Fu, S. M., Winchester, R. J., Rai, K. R., Kunkel, H. G.: Hairy cell leukemia: Proliferation of a cell with phagocytic and B-lymphocyte properties. Scand. J. Immunol.3, 847–851 (1974)

    Google Scholar 

  11. Golde, D. W., Stevens, R. H., Quan, S. G., Saxon, A.: Immunoglobulin synthesis in hairy cell leukaemia. Br. J. Haematol.35, 359–365 (1977)

    Google Scholar 

  12. Grossi, C. E., Webb, S. R., Zicca, A., Lydyard, P. M., Moretta, L., Mingari, M. C., Cooper, M. D.: Morphological and histochemical analyses of two human T-cell subpopulations bearing receptors for IgM or IgG. J. Exp. Med.147, 1405–1417 (1978)

    Google Scholar 

  13. von Heyden, H. W., Waller, H. D., Pape, G. R., Benöhr, H. C., Braun, H. J., Wilms, K., Rieber, E. P., Riethmüller, G.: Haarzell-Leukämie. I. Klinik, Zytochemie, Phagozytosefähigkeit von Haarzellen, Etablierung permanent wachsender Zellinien. Dtsch. Med. Wochenschr.101, 3–8 (1976)

    Google Scholar 

  14. Higgy, K. E., Burns, G. F., Hayhoe, F. G. J.: Identification of the hairy cells of leukaemic reticuloendotheliosis by an esterase method. Br. J. Haematol.38, 99–106 (1978)

    Google Scholar 

  15. Horwitz, D. A., Allison, A. C., Ward, P., Knight, N.: Identification of human mononuclear leucocyte populations by esterase staining. Clin. Exp. Immunol.30, 289–298 (1977)

    Google Scholar 

  16. Jaffe, E. S., Shevach, E. M., Frank, M. M., Green, I.: Leukemic reticuloendotheliosis: Presence of a receptor for cytophilic antibody. Am. J. Med.57, 108–114 (1974)

    Google Scholar 

  17. Knowles, D.M., II, Huffman, T., Ferrarini, M., Kunkel, H. G.: The demonstration of acid α-naphthyl acetate esterase activity in human lymphocytes: Usefulness as a T-cell marker. Cell. Immunol.35, 112–123 (1978)

    Google Scholar 

  18. Kulenkampf, J., Janossy, G., Greaves, M. F.: Acid esterase in human lymphoid cells and leukaemic blasts: a marker for T lymphocytes. Br. J. Haematol.36, 231–240 (1977)

    Google Scholar 

  19. Leder, L.-D.: Der Blutmonocyt. Berlin, Heidelberg, New York: Springer 1967

    Google Scholar 

  20. Leder, L.-D.: Zytochemische Differenzierung normaler und pathologischer mononukleärer Blut- und Knochenmarkszellen. Acta Histochem., Suppl.9, 141–151 (1971)

    Google Scholar 

  21. Löffler, H., Graubner, M., Desaga, J. F., Jung, M.: Prolymphocytic leukemia with T-cell properties and tartrate-resistant acid phosphatase. In: Immunological Diagnosis of Leukemias and Lymphomas. Haematology and Blood Transfusion, Vol. 20. Thierfelder, S. et al., (ed.), pp. 175–178. Berlin, Heidelberg, New York: Springer 1977

    Google Scholar 

  22. Löffler, H., Roux, A., Fischer, J., Desaga, J. F., Pralle, H., Graubner, M.: Hairy cell-Leukämie. In: Maligne Lymphome und monoklonale Gammopathien. Hämatologie und Bluttransfusion, Bd. 18. Löffler, H. (ed.), pp. 255–272. München: Lehmanns 1976

    Google Scholar 

  23. Moretta, L., Webb, S. R., Grossi, C. E., Lydyard, P. M., Cooper, M. D.: Functional analysis of two human T-cell subpopulations: Help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J. Exp. Med.146, 184–200 (1977)

    Google Scholar 

  24. Mueller, J., Brun del Re, G., Buerki, H., Keller, H.-U., Hess, M.W., Cooper, H.: Nonspecific acid esterase activity: A criterion for differentiation of T and B lymphocytes in mouse lymph nodes. Eur. J. Immunol.5, 270–274 (1975)

    Google Scholar 

  25. Naeim, F., Smith, G. S.: Leukemic reticuloendotheliosis. Cancer34, 1813–1821 (1974)

    Google Scholar 

  26. Nanba, K., Jaffe, E. S., Soban, E. J., Braylan, R. C., Berard, C. W.: Hairy cell leukemia. Enzyme histochemical characterization, with special reference to splenic stromal changes. Cancer39, 2323–2336 (1977)

    Google Scholar 

  27. Pangalis, G. A., Waldman, S. R., Rappaport, H.: Cytochemical findings in human nonneoplastic blood and tonsillar B and T lymphocytes. Am. J. Clin. Pathol.69, 314–318 (1978)

    Google Scholar 

  28. Ranki, A., Tötterman, T. H., Häyry, P.: Identification of resting human T and B lymphocytes by acidα-naphthyl acetate esterase staining combined with rosette formation with Staphylococcus aureus strain Cowan 1. Scand. J. Immunol.5, 1129–1138 (1976)

    Google Scholar 

  29. Rieber, E. P., Linke, R. P., Hadam, M., Saal, J. G., Riethmüller, G., v. Heyden, H. W., Waller, H. D., Schwarz, H.: Hairy cell leukemia: Simultaneous demonstration of autochthonous surface-Ig and monocytic functions of hairy cells. In: Immunological Diagnosis of Leukemias and Lymphomas. Haematology and Blood Transfusion, Vol. 20. Thierfelder, S. et al. (ed.), pp. 157–161. Berlin, Heidelberg, New York: Springer 1977

    Google Scholar 

  30. Schaefer, H.-E., Hellriegel, K.-P., Zack, J., Fischer, R.: Zytochemischer Polymorphismus der sauren Phosphatase bei Haarzell-Leukämie. Blut31, 365–370 (1975)

    Google Scholar 

  31. Schnitzer, B., Kass, L.: Hairy-cell leukemia. A clinicopathologic and ultrastructural study. Am. J. Clin. Pathol.61, 176–187 (1974)

    Google Scholar 

  32. Seiler, F. R., Sedlacek, H. H., Kanzy, E. J., Lang, W.: Über die Brauchbarkeit immunologischer Nachweismethoden zur Differenzierung funtionell verschiedener Lymphozyten: Spontanrosetten, Komplementrezeptor-Rosetten und Immunglobulinrezeptoren. Behring Inst. Mitt.52, 26–72 (1972)

    Google Scholar 

  33. Seshadri, R. S., Brown, E. J., Zipursky, A.: Leukemic reticuloendotheliosis. A failure of monocyte production. N. Engl. J. Med.295, 181–184 (1976)

    Google Scholar 

  34. Stein, H.: Immunchemische und immunzytologische Befunde bei Non-Hodgkin-Lymphomen. In: Maligne Lymphome und monoklonale Gammopathien. Hämatologie und Bluttransfusion, Bd. 18. Löffler, H. (ed.), pp. 167–183. München: Lehmanns 1976

    Google Scholar 

  35. Stein, H.: The immunologic and immunochemical basis for the Kiel classification. In: Lennert, K.: Malignant Lymphomas Other than Hodgkin's Disease. Handbuch der speziellen pathologischen Anatomie und Histologie, Bd. I/3B. Uehlinger, E. (ed.), pp. 529–657. Berlin, Heidelberg, New York: Springer 1978

    Google Scholar 

  36. Stein, H., Kaiserling, E.: Surface immunoglobulins and lymphocyte-specific surface antigens on leukaemic reticuloendotheliosis cells. Clin. Exp. Immunol.18, 63–71 (1974)

    Google Scholar 

  37. Stein, H., Müller-Hermelink, H. K.: Stimultaneous presence of receptors for complement and sheep red blood cells on human fetal thymocytes. Br. J. Haematol.36, 227–233 (1977)

    Google Scholar 

  38. Stein, H., Petersen, N., Gaedicke, G., Lennert, K., Landbeck, G.: Lymphoblastic lymphoma of convoluted or acid phosphatase type — a tumor of T precursor cells. Int. J. Cancer17, 292–295 (1976)

    Google Scholar 

  39. Stein, H., Tolksdorf, G.: Die immunologische Basis der Kiel-Klassifikation der malignen Non-Hodgkin-Lymphome. In: Lymphknotentumoren. Stacher, A., Höcker, P. (ed.), pp. 108–115. München, Wien, Baltimore: Urban & Schwarzenberg 1979

    Google Scholar 

  40. Tötterman, T. H., Ranki, A., Häyry, P.: Expression of the acid α-naphthyl acetate esterase marker by activated and secondary T lymphocytes in man. Scand. J. Immunol.6, 305–310 (1977)

    Google Scholar 

  41. Yam, L. T., Li, C. Y., Finkel, H. E.: Leukemic reticuloendotheliosis. The role of tartrateresistant acid phosphatase in diagnosis and splenectomy in treatment. Arch. Intern. Med.130, 248–256 (1972)

    Google Scholar 

  42. Yam, L. T., Li, C. Y., Lam, K. W.: Tartrate-resistant acid phosphatase isoenzyme in the reticulum cells of leukemic reticuloendotheliosis. N. Engl. J. Med.284, 357–360 (1971)

    Google Scholar 

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  1. Institut für Pathologie, Klinikum der Universität, Hospitalstraße 42, D-2300, Kiel 1, Germany

    G. Tolksdorf & H. Stein

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  1. G. Tolksdorf
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  2. H. Stein
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This work was supported by the Deutsche Forschungsgemeinschaft, SFB 111, Project CL1.

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Tolksdorf, G., Stein, H. Acid alpha-naphthyl acetate esterase in hairy cell leukemia cells and other cells of the hematopoietic system. Blut 39, 165–176 (1979). https://doi.org/10.1007/BF01008446

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