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Invertase and phosphatase of yeast in a phosphate-limited continuous culture

  • Kiyoshi Toda
  • Isamu Yabe
  • Toshihiko Yamagata
Biotechnology

Summary

Deficiency of inorganic phosphate caused the hyper production of invertase and the derepression of acid phosphatase in a continuous culture ofSaccharomyces carlsbergensis. The specific invertase activity was 40,000 enzyme units per g dry cell weight at a dilution rate lower than 0.05 h−1 with a synthetic glucose medium of which the molecular ratio of KH2PO4 to glucose was less than 0.006. This activity is eight fold higher than in a batch growth and 1.5 fold as much as the highest enzyme activity observed so far in a glucose-limited continuous culture.

For the hyper production of invertase, it is necessary to culture the yeast continuously by keeping the Nyholm's conservative inorganic phosphate concentration at less than 0.2 m mole per g dry weight cell. The derepression of acid phosphatase brought about by phosphate deficiency, was similar in both batch and continuous cultures.

Keywords

Acid Phosphatase Dilution Rate Continuous Culture Inorganic Phosphate Concentration Cell Weight 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Nomenclature

D

dilution rate of continuous culture (h−1)

Ei

invertase concentration in culture (enzyme unit l−1)

Ep

acid phosphatase concentration in culture (enzyme unit l−1)

P

inorganic phosphate concentration in culture (mM)

S

glucose concentration in culture (mM)

X

cell concentration in culture (g dry weight cell l−1)

Greek Letter

μ

specific rate of growth (h−1)

Suffix

f

feed

0

initial value

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Kiyoshi Toda
    • 1
  • Isamu Yabe
    • 1
  • Toshihiko Yamagata
    • 1
  1. 1.Institute of Applied MicrobiologyUniversity of TokyoTokyo

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