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Weitere Anreicherung eines gewebsspezifischen Inhibitors der Lymphopoese (Lymphozytenchalon?)

Further purification of a tissue specific inhibitor in lymphopoiesis (lymphocyte chalone?)

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Summary

A partially purified calf spleen extract, which exhibits the properties of a lymphocyte chalone (tissue-specific, species non-specific, non-toxic and reversible acting, endogenous inhibitor of cell proliferation) as concerning biological activities, has been described earlier. This fraction has been further purified by ion exchange chromatography with DEAE-Sephacel to a 10000-fold purification. The most active fraction inhibited3H-thymidine incorporation into phytohemagglutinin- or pokeweed-mitogen-stimulated lymphocytes by 86% resp. 70%, when given in a concentration of 100 ng/ml. Ehrlichascites-cells were not inhibited by the same concentration. The inhibiting activity is compared to other published lymphocyte inhibitors.

Zusammenfassung

Ein früher beschriebener, teilweise gereinigter Kalbsmilzextrakt weist bezüglich seiner biologischen Wirkung die Eigenschaften eines Lymphozytenchalons auf: er ist gewebsspezifisch, artunspezifisch, nicht toxisch und inhibiert die Zellproliferation reversibel. Dieser Extrakt wurde durch Ionenaustauschchromatographie mit DEAE-Sephacel aufgetrennt und liegt nun in 10 000-facher Anreicherung vor. Die aktivste Fraktion hemmte den Thymidineinbau von Phytohämagglutinin- oder Pokeweed-Mitogen-stimulierten Lymphozyten um 86% bzw. 70% in einer Konzentration von 100 ng/ml. Ehrlich-Ascites-Zellen wurden in derselben Konzentration nicht gehemmt. Der Hemmstoff wird mit anderen nicht bekanntgewordenen Lymphozyteninhibitoren verglichen.

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Abbreviations

Ü 30:

membrangereinigter Kalbsmilzextrakt (Überstand PM 30 — s. Abb. 1)

RPMI 1640:

Gewebekulturmedium

PHA:

Phytohämagglutinin = pflanzliches Lektin, das T-Lymphozyten stimuliert

PWM:

„Poke weed mitogen“ = pflanzliches Lektin, das B-Lymphozyten stimuliert

Lymphoprep:

fertige Mischung langkettiger Kohlenhydrate zur Zellseparation

DEAE-Sephacel:

perlenförmiges Cellulose-Gel mit Diäthylaminoäthylgruppen als Ionenaustauscher

1 E:

1 Einheit = die Aktivität, die den3H-Thymidineinbau in 5 × 105 proliferierende Lymphozyten um 50% hemmt

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Authors and Affiliations

  1. Abteilung II, Medizinische Universitätsklinik Tübingen, Otfried-Müller-Straße, D-7400, Tübingen, Bundesrepublik Deutschland

    E. Heidemann, J. Masurczak, N. Podgornik, H. Schmidt & K. Wilms

  2. Physiologisch-Chemisches Institut der Universität Tübingen, D-7400, Tübingen, Bundesrepublik Deutschland

    A. Jung

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  1. E. Heidemann
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  2. A. Jung
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  3. J. Masurczak
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  4. N. Podgornik
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  5. H. Schmidt
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  6. K. Wilms
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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft

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Heidemann, E., Jung, A., Masurczak, J. et al. Weitere Anreicherung eines gewebsspezifischen Inhibitors der Lymphopoese (Lymphozytenchalon?). Blut 39, 99–106 (1979). https://doi.org/10.1007/BF01008085

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  • DOI: https://doi.org/10.1007/BF01008085

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