Virchows Archiv

, Volume 430, Issue 1, pp 29–35 | Cite as

Induction of apoptosis in a human hepatocellular carcinoma cell line by a neutralizing antibody to transforming growth factor-α

  • Shuichi Seki
  • Yasuhiko Sakai
  • Takuya Kitada
  • Nobuyoshi Kawakita
  • Atsushi Yanai
  • Hiroko Tsutsui
  • Hiroki Sakaguchi
  • Tetsuo Kuroki
  • Takeyuki Monna
Original Article


A cell line derived from a Japanese man with hepatocellular carcinoma was established in culture and designated OCUH-16. The cell line has the morphological and chromosomal features of hepatocellular carcinoma cells and has a short doubling time (≈33 h). OCUH-16 cells were shown to express transforming growth factor-α, (TGF-α) in addition to albumin, DNA polymerase-α, c-JUN, and the retinoblastoma gene product. Electron microscopy revealed TGF-α immunoreactivity associated with the cell membrane, but TGF-α was not detected in medium conditioned by OCUH-16 cells by enzyme-linked immunosorbent assay. Reverse transcription and polymerase chain reaction analysis revealed the presence of TGF-α messenger RNA in these cells. Culture of OCUH-16 cells in the presence of a neutralizing antibody to TGF-α inhibited cell proliferation and induced many cells to undergo apoptosis (programmed cell death). These observations suggest that endogenous TGF-α is necessary for OCUH-16 cell growth.

Key words

Hepatocellular carcinoma Apoptosis Transforming growth factor-α 


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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Shuichi Seki
    • 1
  • Yasuhiko Sakai
    • 1
  • Takuya Kitada
    • 1
  • Nobuyoshi Kawakita
    • 1
  • Atsushi Yanai
    • 1
  • Hiroko Tsutsui
    • 2
  • Hiroki Sakaguchi
    • 1
  • Tetsuo Kuroki
    • 1
  • Takeyuki Monna
    • 3
  1. 1.Third Department of Internal MedicineOsaka City University Medical SchoolOsakaJapan
  2. 2.Third Department of BiochemistryOsaka City University Medical SchoolOsakaJapan
  3. 3.Department of Public HealthOsaka City University Medical SchoolOsakaJapan

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