Skip to main content

Advertisement

SpringerLink
Log in

DNA-synthesizing T and non-T cells in chronic lymphocytic leukemia

  • Original Works
  • Published:
Blut Aims and scope Submit manuscript

Zusammenfassung

Bei 21 Patienten mit chronischer lymphatischer LeukÄmie (CLL) und 8 Normalpersonen wurde die Anzahl der DNA-synthetisierenden peripheren Blutlymphozyten autoradiografisch untersucht. Die Lymphozyten wurden dabei mit Hilfe des EN-Rosettentests in T-Zellen und Nicht-T-Zellen differenziert. Es zeigt sich eine normale T-Zellproliferation und eine erhöhte Nicht-T-Zellproliferation im Stadium O-I. In den fortgeschritteneren Krankheitsstadien erfÄhrt die Proliferation für T- und Nicht-T-Lymphozyten eine signifikante Steigerung. Die Proliferation der T-Zellen ist im Stadium IV auf das ca. 20fache, die der Nicht-T-Zellen auf das ca. 50fache gesteigert. Auf die Möglichkeit zu prognostischen Aussagen wird hingewiesen.

Summary

In 21 patients with chronic lymphocytic leukemia (CLL) and in 8 hematologically normal persons the number of DNA-synthesizing peripheral blood lymphocytes was investigated by autoradiographic techniques. The lymphocytes were differentiated by En-rosette tests into T and non-T lymphoid cells. The results show a normal number of proliferating T lymphoid cells and an increased number of proliferating non-T lymphoid cells in clinical stages O-I. Stages III–IV demonstrate a significant increase of the proliferation rate of both T and non-T lymphoid cells. The possible pathogenetic factors and the prognostic value of these results are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Alexopoulos, C., Babitis, P.: Age dependence of T lymphocytes. LancetI, 426 (1976)

    Google Scholar 

  2. Augener, W., Cohnen, G., Reuter, A., Brittinger, G.: Decrease of T lymphocytes during ageing. LancetI, 1164 (1974)

    Google Scholar 

  3. Begemann, H., Rastetter, J., Kaboth, W.: Klinische HÄmatologie, 2nd ed., pp. 583–606. Stuttgart: G. Thieme 1975

    Google Scholar 

  4. Bentwich, Z., Polliack, A., Douglas, S. D.: Surface markers and other characteristics of the lymphocyte in chronic lymphocytic leukaemia. Isr. J. Med. Sci.12, 304–324 (1976)

    Google Scholar 

  5. Catovsky, D., Miliani, E., Okos, A., Galton, D. A. G.: Clinical significance of T-celis in chronic lymphocytic leukaemia. LancetII, 751–752 (1974)

    Google Scholar 

  6. Crowther, D., Fairley, G. H., Sewell, R. L.: Significance of the changes in the circulating lymphoid cells in Hodgkin's disease. Brit. Med. J.2, 473–477 (1969)

    Google Scholar 

  7. Crowther, D., Fairley, G. H., Sewell, R. L.: Lymphoid cellular responses in the blood after immunization in man. J. Exp. Med.129, 849–862 (1969)

    Google Scholar 

  8. Diaz-Jouanen, E., Williams, R. C., Strickland, R. G.: Age related changes in T and B cells. LancetI, 688–689 (1975)

    Google Scholar 

  9. Huber, Ch., Michlmayr, G., Falkensamer, M., Fink, U., Zur Nedden, G., Braunsteiner, H., Huber, H.: Increased proliferation of T lymphocytes in the blood of patients with Hodgkin's disease. Clin. Exp. Immunol.21, 47–53 (1975)

    Google Scholar 

  10. Huber, Ch., Huber, H., Schmalzl, F., Lederer, B., Bütterich, D., Braunsteiner, H.: DNS-Synthese in Blutlymphozyten beim malignen Lymphogranulom. Acta Haemat.44, 222–232 (1970)

    Google Scholar 

  11. Jondal, M., Holm, G., Wigzell, H.: Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J. Exp. Med.136, 207–215 (1972)

    Google Scholar 

  12. Knospe, W. H., Loeb, V. Jr., Huguley, C. M. Jr.: Proceeding: Bi-weekly chlorambucil treatment of chronic lymphocytic leukaemia. Cancer33, 555–562 (1974)

    Google Scholar 

  13. Lopez Sandoval, R., Moayeri, H., Sokal, J. E.: In vitro leukocyte thymidine uptake in chronic lymphocytic leukaemia. Cancer Res.34, 146–150 (1974)

    Google Scholar 

  14. Michlmayr, G., Pathouli, Ch., Falkensamer, M., Huber, Ch., Huber, H., Braunsteiner, H.: Rosettentests bei lymphoproliferativen Erkrankungen. Schweiz. Med. Wschr.106, 794–799 (1976)

    Google Scholar 

  15. Nowell, P., Daniele, R., Rowlands, D. Jr. Winger, L.: T and B lymphocytes in chronic lymphocytic leukaemia. New Engl. J. Med.295, 9, 504 (1976)

    Google Scholar 

  16. Rai, K. R., Sawitzky, A., Cronkite, E. P., Chanana, A. D., Levy, R. N., Pasternack, B. S.: Clinical staging of chronic lymphocytic leukaemia. Blood46, 219–234 (1975)

    Google Scholar 

  17. Schick, P., Trepel, F., Theml, H., Benedek, S., Trumpp, P., Kaboth, W., Begemann, H., Fliedner, T. M.: Kinetics of lymphocytes in Hodgkin's disease. Blut27, 223–235 (1973)

    Google Scholar 

  18. Stjernsward J., Vánky, F., Jondal, M., Wigzell, H., Sealy, R.: Lymphopenia and charge in distribution of human B and T lymphocytes in peripheral blood induced by irradiation for mammary carcinoma. LancetI, 1352–1356 (1972)

    Google Scholar 

  19. Theml, H., Trepel, F., Schick, P., Kaboth, W., Begemann, H.: Kinetics of lymphocytes in chronic lymphocytic leukaemia: Studies using contineous3H-thymidine infusion in two patients. Blood42, 623–636 (1973)

    Google Scholar 

  20. Wybran, J., Chantier, S., Fudenberg, H. H.: Isolation of normal T cells in chronic lymphocytic leukaemia. LancetI, 126 (1973)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. StÄdtisches Krankenhaus Schwabing, I. Medizinische Abteilung, Kölner Platz l, D-8000, München 40, Germany

    J. A. van der Woerd-de Lange, J. Döhrmann, K. Kauert & H. Begemann

  2. Medizinische UniversitÄtsklinik, A-6020, Innsbruck, Austria

    Ch. Huber

  3. Abt. Klinische Morphologie der UniversitÄt, D-7900, Ulm, Germany

    P. Schick

Authors
  1. J. A. van der Woerd-de Lange
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Döhrmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ch. Huber
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Schick
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. Kauert
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. H. Begemann
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by the Deutsche Forschungsgemeinschaft (Be 79/15) and by the Austrian Funds „Zur Förderung der wissenschaftlichen Forschung“ and „Kampf dem Krebs“

Rights and permissions

Reprints and permissions

About this article

Cite this article

van der Woerd-de Lange, J.A., Döhrmann, J., Huber, C. et al. DNA-synthesizing T and non-T cells in chronic lymphocytic leukemia. Blut 37, 319–326 (1978). https://doi.org/10.1007/BF01007867

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01007867

Schlüsselwörter

Key words

Search

Navigation