, Volume 37, Issue 6, pp 313–317 | Cite as

Evaluation of non-stimulated nitroblue-tetrazolium test in patients with malignant lymphomas

  • V. Liso
  • G. Specchia
  • G. Troccoli
Original Works


The NBT-test was performed in 54 patients with Hodgkin and nonHodgkin lymphoma showing no bacterial infection exept for one case. 18 patients presented, at onset of the disease, a number of neutrophilic NBT-reducing granulocytes at pathological levels (> 12%).

In order to evaluate the influence of cytostatic drugs on NBT-test serial blood samples were taken during polychemotherapy in 17 patients with lymphoma. In our experience, cytostatics (COPP) do not necessarily lessen NBT-reducing activity of granulocytes.

The results suggest that the NBT in malignant lymphomas could not be used as an indication of bacterial infection.

Key words

NBT-test Malignant lymphomas 

Die Bewertung des NBT-Testes bei malignen Lymphomen


Der Nitroblau-Tetrazolium-Test (NBT) wurde bei 54 Patienten mit malignen Lymphomen durchgeführt. Diese Patienten hatten mit einer Ausnahme keine erkennbaren Infektionen. Bei 18 Patienten war vorder Erkrankung die Zahl der NBT-reduzierenden Granulozyten über die Norm erhöht.

Verlaufsuntersuchungen wÄhrend einer Polychemotherapie bei 17 Patienten zeigten, da\ die Zytostatika-Therapie als solche die Zahl der NBT-reduzierten Granulozyten nicht vermindert.

Die Ergebnisse zeigen, da\ der NBT-Test bei malignen Lyphomen zum Nachweis bakterieller Infektionen nicht geeignet ist.


NBT-Test Maligne Lymphome 


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  1. 1.
    Editorial: Another look at the NBT-test. LancetI, 664–665 (1974)Google Scholar
  2. 2.
    Feigin, R. D., Shackelford, P. G., Choi, Flake K. K., Franklin, F. A., Eisenberg, C. S.: Nitroblue tetrazolium dye test as an aid in the differential diagnosis of febrile disorders. J. Pediatr.78, 230–237 (1971)Google Scholar
  3. 3.
    Gordon, A. M., Rowman, R. M., Browt, Carson, H. C.: Routine application of the nitroblue tetrazolium test in the clinical laboratory. J. Clin. Pathol.26, 52–56 (1973)Google Scholar
  4. 4.
    Matula, G., Paterson, P. Y.: NBT-tests in a patient on steroids. LancetI, 803–804 (1971)Google Scholar
  5. 5.
    Matula, G., Peterson, P. Y.: Spontaneous in vitro reduction of nitroblue tetrazolium by neutrophils of adult patients with bacterial infection. N. Engl. J. Med.283, 311–317 (1971)Google Scholar
  6. 6.
    Miller, R. M., Garbus, J., Schwartz, R., DuPont, H. L., Levine, M. M., Clyde, D. F., Hornick, R. B.: A modified leukocyte nitroblue tetrazolium test in acute bacterial infection. Am. J. Clin. Pathol.66, 905–910 (1976)Google Scholar
  7. 7.
    Park, M. B., Firking, S. M., Smithwich, E. M.: Infection and nitroblue tetrazolium reduction by neutrophils, a diagnostic aid. LancetII, 532–534 (1968)Google Scholar
  8. 8.
    Silvermann, P. M., Reed, R. E.: The nitrobule tetrazolium test in lymphoma. Am. J. Clin. Pathol.60, 198–201 (1973)Google Scholar
  9. 9.
    Steighigel, R. T., Johnson, P. K., Remington, I. S.: The nitroblue tetrazolium reduction test versus conventional hematologie in the diagnosis of bacterial infection. N. Engl. J. Med.290, 235–238 (1974)Google Scholar
  10. 10.
    Troccoli, G., Specchia, G., Grande, M., Liso, V.: Sulla valutazione del test al nitroblue di tetrazolio. Atti della Società Italiana di Ematologia2, 51–54 (1976)Google Scholar
  11. 11.
    Wantzin, G. L., Wantzin, J.: The NBT test: erratic behaviour in acute leukaemia. Biut31, 133–142 (1975)Google Scholar
  12. 12.
    Wantzin, G. L.: The effect of prednisone on the non-stimulated and the stimulated NBT test. Biomedicine22, 122–127 (1975)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • V. Liso
    • 1
  • G. Specchia
    • 1
  • G. Troccoli
    • 1
  1. 1.Istituto di Clinica Medica II, Sez. EmatologiaUniversitá di BariBariItaly

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