Synopsis
The distribution of succinate dehydrogenase in the odontogenic tissues in the Mongolian gerbil, ranging in age from 18 days prenatal to 8 days postnatal, was studied. Activity levels were designated negative, trace, slight, moderate, strong and very strong, dependent upon visual observations. The results indicated increased levels of activity as the tissue layers differentiated from the lamina stage through early apposition. The cell layer displaying the most succinate dehydrogenase activity was the ameloblastic layer while the stratum intermedium layer was the, next most active cell layer. Succinate dehydrogenase activity appeared to be related to the degree of differentiation and functional competency of the odontogenic tissues in the Mongolian gerbil.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Afonsky, D. (1957). Dental caries in the Mongolian gerbil.N. Y. St. Dent. J. 23, 315–16.
Allanson, J. (1970). Gerbils. In:Reproduction, and Breeding Techniques for Laboratory Animals (ed.) E. S. E. Hafez) Ch. 13. Philadelphia: Lea & Febiger.
Atkinson, E., Melvin, S. &Fox, S. W. (1950) Some properties of 2, 3, 5-triphenyltetrazolium chloride and several iodo derivatives.Science 111, 385–7.
Balogh, K. (1963). Histochemical study of oxidative enzyme systems in teeth and periodontal tissues.J. dent. Res. 42, 1457–66.
Burstone, M. S. (1960). Histochemical observations on enzymatic processes in bones and teeth.Ann. N.Y. Acad. Sci. 85, 431–44.
Fitzgerald, D. B. &Fitzgerald, R. J. (1965). Induction of dental caries in gerbils.Archs. oral Biol. 11, 139–40.
Frank, R. M. &Nalbandian, J. (1967). Ultrastructure of amelogenesis. In:Structural and Chemical Organization of Teeth (ed. A. E. W. Miles). New York: Academic Press.
Fullmer, H. M. (1963). Dehydrogenases in developing teeth of rats.J. Histochem. Cytochem. 11 641–64.
Fulimer, H. M. (1964). The use of histochemistry in oral histology. In:Oral Histology-Inheritance and Development (ed. D. V. Provenza). Philadelphia: Lippincott.
Goggins, J. F. &Fullmer, H. M. (1966). Dehydrogenase histochemistry of the rat molar pulp.Archs oral Biol. 11, 1365–70.
Gupta, O. P. &Shaw, J. H. (1960). Dental Anatomy and characteristics of periodontal lesions in the Mongolian gerbil.J. dent. Res. 39, 1014–22.
Hiatt, J. L., Gartner, L. P. &Provenza, D. V. (1974). Molar development in the Mongolian gerbil (Meriones unguiculatus).Am. J. Anat. 141, 1–22.
Hiatt, J. L., Gartner, L. P., Swartz, W. J. &Provenza, D. V. (1974). Use of a photographic method for rapid evaluation of histological sections.Trans. Am. micros. Soc. 93, 118–19.
Kramer, A. W., Jr. (1964) Body and organ weights and linear measurements of the adult Mongolian gerbil.Anat. Rec. 150, 343–8.
Lefkowitz, W., Bodecker, C. F. &Mardfin, D. F. (1953). Odontogenesis of the rat molar.J. dent. Res. 32, 749–72.
Lehniger, A. L. (1965).The Mitochondrion. New York: Benjamin.
Loew, M. (1967). A case of overgrown mandibular incisors in a Mongolian gerbil.Lab. Anim. Care 17, 137–9.
Mahler, H. R. &Cordes, E. H. (1968).Biological Chemistry. New York: Harper & Row.
Marston, J. H. &Chang, M. C. (1965). The breeding, management and reproductive physiology of the Mongolian gerbil (Meriones unguiculatus).Lab. Anim. Care 15, 34–48.
Matthiessen, M. E. (1968) Comparative enzyme histochemical studies on the development of teeth in man and mouse.Histochemie 13, 238–44.
Mays, A., Jr. (1969). Baseline hematological and blood biochemical parameters of the Mongolian gerbil (Meriones unguiculatus).Lab. Anim. Care 19, 838–42.
Mcewen, B. S., Allfrey, V. G. &Mirsky, A. E. (1963). Studies on energy-yielding reactions in thymus nuclei. II. Pathways of aerobic carbohydrate, catabolism.J. biol. Chem. 238, 2571–8.
Meckley, P. E. &Ginther, O. J. (1972). Effects of litter and male on corpora lutea of the postpartum Mongolian gerbil.J. Anim. Sci. 34, 297–301.
Mizushima, T. (1964). Histochemical studies of dehydrogenases in the developing teeth.Histochemie 3, 538–51.
Mori, M., Mizushima, T. &Osanai, K. (1961). The histochemical demonstration of succinic dehydrogenase activity in developing teeth.Histochemie 2, 348–55.
Moskow, B. S., Wasserman, B. H. &Rennert, M. C. (1968). Spontaneous periodontal disease in the Mongolian gerbil.J. periodont. Res. 3, 69–83.
Nachlas, M. M., Tsou, K. C., Souza, E. D., Chang, C. S. &Seligman, A. M., (1957). Cytochemical demonstration of succinic dehydrogenase by the use of a newp-nitrophenyl substituted ditetrazole.J. Histochem. Cytochem. 5, 420–36.
Norris, M. L. &Adams, C. E. (1971). Delayed implantation in the Mongolian gerbil (Meriones unguiculatus).J. Reprod. Fert. 27, 486–7.
Pearson, B. (1958). Improvement in the histochemical localization of succinic dehydrogenase by use of nitroneotetrazolium chloride.J. Histochem. Cytochem. 6 112–21.
Pourtois, M. (1962). La minéralisation de la dentine et de l'émail dans ses rapports avec le métabolisme des tissus, générateurs.Arch. Biol., Paris 73, 491–520.
Provenza, D. V. (1964).Oral Histology—Inheritance and Development. Philadelphia: Lippincott.
Provenza, D. V., Chang, Y. F., Gartner, L. P. &Garlitz, R. M. (1972). Histochemical demonstration of succinic dehydrogenase activity during sequential molar development in the Swiss albino mouse.Histochemie 29, 355–61.
Quintarelli, G. (1961). Enzyme histochemistry of tooth germs.Arch. ital. Biol. Orale. 2, 1–25.
Reith, E. J. &Zussman, W. V. (1964). Succinic dehydrogenase in ameloblasts during formation and maturation of enamel.Arch. oral Biol. 9, 31–8.
Rich, S. T. (1968). The Mongolian gerbil (Meriones, unguiculatus) in research.Lab. Anim. Care 18, 235–43.
Schwentker, V. (1963). The gerbil, a new laboratory animal.Illinois Vet. 6, 5–9.
Schwentker, V. (1971).An annotated Bibliography of the Gerbil as an Experimental Animal in Medical Research. Tumblebrook Farm., Brant Lake, New York.
Seligman, A. M. (1973). Personal communication.
Shelton, E. &Schneider, W. C. (1952). On the usefulness of tetrazolium salts as histochemical indicators of dehydrogenase activity.Anat. Rec. 112, 61–81.
Singer, T. P. &Kearney, E. B. (1963). Succinic dehydrogenase. In:The Enzymes (eds. P. D. Boyer, H. Lardy & K. Myrback), Vol. 7, Ch. 16. New York: Academic Press.
Swartz, E. M. &Yaeger, J. A. (1961). Succinic dehydrogenase activity in odontoblasts.J. dent. Res. 40, 1291.
Ten Cate, A. R. (1967). A histochemical study of the human, odontoblast.Archs. oral Biol. 12, 963–9.
Thomas, E. &Pearse, A. G. E. (1961). The fine localization of dehydrogenases in the nervous system.Histochemie,2, 226–82.
Wasserman, B. H., Moskow, B. S. &Rennert, M. C. (1970). Dental anatomy and coronal cementum in the Mongolian gerbil.J. periodont. Res. 5, 208–18.
White, A., Handler, P. &Smith, E. L. (1968).Principles of Biochemistry. New York: McGraw-Hill.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hiatt, J.L. Succinate dehydrogenase activity in the developing molar of the Mongolian gerbilMeriones unguiculatus . Histochem J 8, 103–112 (1976). https://doi.org/10.1007/BF01007162
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01007162