Skip to main content
SpringerLink
Log in

Leaching of glycosaminoglycans from tissues by the fixatives formalin-saline and formalin-cetrimide

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Synopsis

The solubility of acid glycosaminoglycans in fixatives varies with the tissue source: it appears to be much greater in intervertebral disc than in skin. Presence of Cetrimide in the fixative reduces, but does not abolish, leaching in disc and has little or no effect on skin glycosaminoglycans.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Antonopoulos, C. A., Gardell, S., Szirmai, J. A. &Detyssonsk, E. R. (1964). Determination of glycosaminoglycans (mucopolysacharides) from tissues on the microgram scale.Biochim. biophys. Acta 83, 1–19.

    Google Scholar 

  • Butler, W. F. (1974). Comparison of the effects of early and late fixation on Alcian Blue staining in dog skin using two different fixativesHistochem. J. 6, 463–5.

    Google Scholar 

  • Butler, W. F., &Wels, C. (1971). Glycosaminoglycans of cat intervertebral disc.Biochem. J. 122, 647–52.

    Google Scholar 

  • Conklin, J. L. (1963). Staining reaction of mucopolysaccharides after formalin containing fixatives.Stain Technol. 38, 56–9.

    Google Scholar 

  • Gardell, S. &Szirmai, J. A. (1967).The Connective Tissues Chapter 19. Baltimore: Williams & Wilkins.

    Google Scholar 

  • Kelly, J. W., Bloom, G. D. &Scott, J. E. (1963). Quaternary ammonium compounds in connective tissue histochemistry.J. Histochem. Cytochem. 41, 791–7.

    Google Scholar 

  • Pearce, R. H. &Grimmer, B. J. (1972). Age and the chemical constitution of normal human dermis.J. invest. Derm. 58, 347–61.

    Google Scholar 

  • Pearson, C. H. (1963). The carbohydrate constituents of bovine skin.J. Soc. Leather Trades Chemists 47, 439–52.

    Google Scholar 

  • Scott, J. E. (1960). Aliphatic ammonium salts in the assay of acidic polysaccharides from tissues.Meth. biochem. Anal. 8, 145–97.

    Google Scholar 

  • Whiteman, P. (1973). The quantitative measurements of Alcian Blue-glycosaminoglycan complexes.Biochem. J. 131, 343–50.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, University of Bristol, Park Row, Bristol, UK

    I. Pousty, M. A. Bari-Khan & W. F. Butler

Authors
  1. I. Pousty
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Bari-Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. F. Butler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pousty, I., Bari-Khan, M.A. & Butler, W.F. Leaching of glycosaminoglycans from tissues by the fixatives formalin-saline and formalin-cetrimide. Histochem J 7, 361–365 (1975). https://doi.org/10.1007/BF01007021

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01007021

Keywords

Search

Navigation