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Arylsulphatase-containing granules in rabbit polymorphonuclear leukocytes

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Summary

Arylsulphatase reactivity was localized to different-sized granules of the rabbit polymorphonuclear leukocyte. The reactivity at pH 7.0 was restricted to small granules (diameter 299 nm) that were usually peripheral in the cell. At pH 5.0, dense, uniform reaction product was found in granules of diameters 299, 516 and 712 nm. At pH 4.2, although granules of all sizes contained reaction product, it was predominantly in the 712 nm diameter granules. Reactivity in monocytes was confined to ovoid or tubular-shaped granules. No reactivity was observed at pH 9.0.

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References

  • Badwey, J. A., Curnette, J. T., Robinson, J. M., Lazdins, J. K., Briggs, R. T., Karnovsky, M. J. &Karnovsky, M. L. (1980) Comparative aspects of oxidative metabolism of neutrophils from human blood and guinea pig peritoneal; magnitude of the respiratory burst, dependence upon stimulating agents, and localization of the oxidases.J. Cell Physiol. 105, 541–51.

    Google Scholar 

  • Bainton, D. F. &Farquhar, M. G. (1968a) Differences in enzyme content of azurophil and specific granules of polymorphonuclear leukocytes. I. Histochemical staining of bone marrow smears.J. Cell Biol. 39, 286–98.

    Google Scholar 

  • Bainton, D. F. &Farquhar, M. G. (1968b) Differences in enzyme content of azurophil and specific granules of polymorphonuclear leukocytes. II. Cytochemistry and electron microscopy of bone marrow cells.J. Cell Biol. 39, 299–317.

    Google Scholar 

  • Bainton, D. F., Ullyot, J. L. &Farquhar, M. G. (1971) The development of neutrophilic polymorphonuclear leukocytes in human bone marrow. Origin and content of azurophil and specific granules.J. Exp. Med. 134, 907–34.

    Google Scholar 

  • Bentwood, B. J. &Henson, P. M. (1980) The sequential release of granule constituents from human neutrophils.J. Immunol. 124, 855–62.

    Google Scholar 

  • Bretz, U. &Baggiolini, M. (1974) Biochemical and morphological characterization of azurophil and specific granules of human neutrophilic polymorphonuclear leukocytes.J. Cell Biol. 63, 251–69.

    Google Scholar 

  • Cohn, Z. A. &Hirsch, J. G. (1960). The isolation and properties of the specific cytoplasmic granules of rabbit polymorphonuclear leukocytes.J. Exp. Med. 112, 983–1004.

    Google Scholar 

  • Dubois, G. &Baumann, N. (1973) Arylsulfatases A and B of human leukocytes; specific inhibitors and electrophoretic characterization.Biochem. Biophys. Res Commun. 50, 1129–35.

    Google Scholar 

  • Geddes, A. D., Kirchen, M. R. &Marshall, G. J. (1975). Localization of leukocyte alkaline phosphatase in human neutrophils.Acta haemat. 53, 145–51.

    Google Scholar 

  • Goldfischer, S. (1965) The cytochemical demonstration of lysosomal arylsulfatase activity by light and electron microscopy.J. Histochem. Cytochem. 13, 520–3.

    Google Scholar 

  • Hopsu-Havu, V. K., Arstila, A. V., Helminen, H. J. &Kalimo, H. O. (1967) Improvements in the method for the electron microscopic localization of arylsulfatase activity.Histochemie 8, 54–64.

    Google Scholar 

  • Hopsu-Havu, V. K. &Helminen, H. (1974) Sulfatases. InElectron Microscopy of Enzymes, Vol. 2 (edited byHayat, M. A.), pp. 90–109. New York:Van Nostrand Reinhold Co.

    Google Scholar 

  • Hugon, J. &Borgers, M. (1967) Fine structural localization of lysosomal enzymes in the absorbing cells of the duodenal mucosa of the mouse.J. Cell Biol. 33, 212–8.

    Google Scholar 

  • Leznicki, A. &Bleszynshi, W. (1970) Histochemical localization of the soluble arylsulfatase activities in rat brain.Histochemie 24, 251–65.

    Google Scholar 

  • Murata, F. &Spicer, S. S. (1973) Morphologic and cytochemical studies of rabbit heterophilic leukocytes. Evidence for tertiary granules.Lab. Invest. 29, 65–72.

    Google Scholar 

  • Nakatsui, T. (1969) Ultrastructural localization of alkaline phosphatase in human neutrophils.Acta haemat. 42, 275–80.

    Google Scholar 

  • Segal, A. W., Dorling, J. &Coade S. (1980) Kinetics of fusion of the cytoplasmic granules with phagocytic vacuoles in human polymorphonuclear leukocytes. Biochemical and Morphological studies.J. Cell Biol. 85, 42–59.

    Google Scholar 

  • Shannon, W. A. Jr, Rockholt, D. &Bates S. B. (1982) Computer-assisted measurements of the thickness of biological structures.Comput. Biol. Med. 12, 149–55.

    Google Scholar 

  • Shannon, W. A. Jr &Zellmer, D. M. (1981) A new species of lysosome in rabbit PMN leukocytes.J. Histochem. Cytochem. 29, 1099–101.

    Google Scholar 

  • Spitznagel, J. K., Dalldorf, F. G., Leffell, M. S., Folds, J. D., Welsh, I. R. H., Cooney, M. H. &Martin, L. E. (1974). Character of azurophil and specific granules purified from human polymorphonuclear leukocytes.Lab. Invest. 30, 774–85.

    Google Scholar 

  • West, B. C., Rosenthal, A. S., Gelb, N. A. &Kimball, H. R. (1974) Separation and characterization of human neutrophil granules.Am. J. Path. 77, 41–61.

    Google Scholar 

  • Zellmer, D. M. &Shannon, W. A. Jr (1980a) Heterogeneity of phosphatase-containing granules in rabbit neutrophils.Texas Soc. Electron Microsc. J. 11, 27–30.

    Google Scholar 

  • Zellmer, D. M. &Shannon, W. A. Jr (1980b) The presence of catalase and myeloperoxidase activity in small granules of rabbit polymorphonuclear leukocytes.Texas Soc. Electron Microsc. J. 11, 38.

    Google Scholar 

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Shannon, W.A., Zellmer, D.M. Arylsulphatase-containing granules in rabbit polymorphonuclear leukocytes. Histochem J 15, 201–209 (1983). https://doi.org/10.1007/BF01006236

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  • DOI: https://doi.org/10.1007/BF01006236

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