Summary
Macrophages activatedin vivo withCorynebacterium parvum and asbestos showed an increased sub-population of cells which capped spontaneously on incubation with fluoresceinated Concanavalin A compared to saline-induced control macrophages. This capping was unaffected by colchicine but was inhibited by cytochalasin B. The spontaneous capping of activated macrophages did not appear to be directly related to the total number of Concanavalin A receptors as measured by specific125I-labelled Concanavalin A binding to activated and control macrophages. Capping also did not appear to be related to the position of Concanavalin A receptors as judged by trypsin accessibility of bound125I-labelled Concanavalin A.
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References
Adachi, I., Katagiri, T., Terao, T., &Osawa, T. (1980) Concanavalin A receptor proteins of plasma membranes of guinea pig macrophages.Cell Struct. Funct.,5, 247–57.
Allison, A. C., Ferluga, J., Prydz, H. &Schorlemmer, H. U. (1978) The role of macrophage activation in chronic inflammation.Agents Actions 8, 27–34.
Cohn, Z. A. (1978) The activation of mononuclear phagocytes. Fact, fancy and future.J. Immun. 121, 813–6.
David, J. R., Remold, H. G., Higgins, T. J., Liu, D. Y. & Churchill, W. H. MIF/MAF-macrophage interactions. Biochemical characterization of a putative glycolipid receptor for MIF and the existence and properties of two distinct MIFs. InMononuclear Phagocytes Functional Aspects, Part I (edited byVan Furth, R.), pp. 527–535. The Hague, Boston, London: Nijhoff.
De Petris, S. (1974) Inhibition and reversal of capping by cytochalasin B, vinblastine and colchicine.Nature, Lond. 250, 54–6.
Donaldson, K., Davis, J. M. G. & James, K. (1982) Characteristics of peritoneal macrophages induced by asbestos.Environmental Res. (in press).
Goldstein, B. D., Hamburger, S. J., Falk, G. W. &Amoroso, M. A. (1977) Effect of ozone and nitrogen dioxide on the agglutination of rat alveolar macrophages by Concanavalin A.Life Sci. 21, 1637–44.
Hadley, J. G., Gardner, D. C., Coffin, D. L. &Menzel, D. B. (1977) Enhanced binding of autologous red cells to the macrophage plasma membrane as a sensitive indicator of pollutant damage. InPulmonary Macrophage and Epithelial Cells (edited bySaunders, C. L., Schneider, P. P., Dagle, G. E. andRegan, H. A.), pp. 66–77. New York: ERDA.
Hammond, M. E. &Dvorak, H. F. (1972) Antigen induced stimulation of glucosamine incorporation by guinea pig peritoneal macrophages in delayed hypersensitivity.J. exp. Med. 136, 1518–32.
Homma, Y., Onozaki, K., Hashimoto, T., Miura, K., Nagao, S. &Tanaka, A. (1981) Different effect of (l) fucose binding lectin on macrophage migration inhibition caused by guinea pig migration inhibition factor and synthetic muramyl dipeptide.Int. Arch. Allerg. appl. Immun. 65, 27–33.
Hopper, K. E., Wood, P. C. &Nelson, D. S. (1979) Macrophage heterogeneity.Vox. Sang. 36, 257–74.
Hunter, W. M. (1973) Radioimmunoassay. InHandbook of Experimental Immunology (edited byWeir, D. M.), pp. 17.1–17.36. Edinburgh: Blackwell.
James, K., McBride, B. &Stuart, A. E. (1977) (editors)The Macrophage and Cancer. Edinburgh: James, McBride and Stuart.
Lipscomb, M. F., Toews, G. B., Lyons, C. R. &Uhe, J. W. (1981) Antigen presentation by guinea pig alveolar macrophages.J. Immun. 126, 286–91.
Loor, F. &Roelants, G. E. (1974) The dynamic state of the macrophage plasma membrane. Attachment and fate of immunoglobulin, antigen and lectins.Eur. J. Immun. 4, 649–60.
Lutton, J. D. (1973) The effect of phagocytosis and spreading on macrophage surface receptors for Concanavalin A.J. Cell Biol.,56, 611–7.
Mantovani, B. (1981) Phagocytosis of immune complexes mediated by IgM and C3 receptors by macrophages from mice treated with glycogen.J. Immun. 226, 127–30.
Marques, E. (1978) Binding of Concanavalin A by normal and transformed cells and by their trypsin released surface components.Expl Cell Res. 115, 143–9.
Miller, K. (1978) The effects of asbestos on macrophages.CRC Crit. Rev. Toxicol. 5, 319–54.
Morahan, P. S. (1980) Macrophage nomenclature; where are we going?J. Reticuloendothel. Soc. 27, 223–45.
Nabarra, B., Cavelier, J. F., Dy, M. &Dimitriu, A. (1978) Scanning electron microscope studies of activated macrophages in the mouse.J. Reticuloendothel. Soc. 24, 489–98.
Nathan, C. F. &Root, R. K. (1977) Hydrogen peroxide release from mouse peritoneal macrophages,J. exp. Med. 246, 1648–62.
Ogmundsdottir, H. &Weir, I. M. (1980) Mechanism of macrophage activationClin. exp. Immun.,40, 223–4.
Oliver, J. M. &Berlin, R. D. (1975) Macrophage membranes. InImmunobiology of the Macrophage (edited byNelson, D. S.), pp. 259–273. London, New York: Academic Press.
Oliver, J. M., Berlin, R. D., Baehner, R. L. &Boxer, L. A. (1977) Mechanism of microtubule disassemblyin vivo; studies on normal and chronic granulomatous disease leucocytes.Br. J. Haemat. 37, 311–22.
Pick, E. &Wilner, I. (1979) Cytoskeletal control of Concanavalin A receptor mobility in peritoneal macrophages.Expl Cell Res. 118, 151–8.
Rabinowitz, M. &Hamburg, S. I. (1980) Macrophage activation by interferon or interferon inducers. InMononuclear Phagocytes, Functional Aspects, Part II (edited byVan Furth, R.), pp. 1515–1536. The Hague, Boston, London: Nijhoff.
Rhodes, J. (1975) Macrophage heterogeneity in receptor activity — the activation of macrophage Fc receptor functionin vivo andin vitro.J. Immun. 114, 976–81.
Rosenthal, A. S., Blake, J. T., Ellner, J. J., Greinedar, I. K. &Lipsky, P. E. (1976) Macrophage function in antigen recognition by T lymphocytes. InImmunobiology of the Macrophage (edited byNelson, D. S.), pp. 131–160, New York, London: Academic Press.
Unanue, E. R. &Rosenthal, A. S. (1980) (editors)Macrophage Regulation of Immunity. New York, London: Academic Press.
Walters, M. N. &Papadimitriou, J. M. (1978) Phagocytosis — a review.CRC Crit. Rev. Toxicol. 5, 377–421.
Williams, D. A., Boxer, L. A., Oliver, J. M. &Baehner, R. L. (1977) Cytoskeletal regulation of Concanavalin A capping in pulmonary alveolar macrophages.Nature, Lond.,267, 255–7.
Williams, D. A., Lessard, J., Boxer, L. A., Rein, D. &Baehner, R. L. (1979) Metabolic and structural requirements for Concanavalin A capping in phagocytic cells.Proc. Soc. exp. Biol. Med. 162, 34–7.
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Donaldson, K., Davis, J.M.G. & James, K. Concanavalin A receptors and capping in control and activated macrophages. Histochem J 15, 59–69 (1983). https://doi.org/10.1007/BF01006071
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DOI: https://doi.org/10.1007/BF01006071