Skip to main content
SpringerLink
Log in

Comparative immunohistochemistry and histochemistry of dipeptidyl peptidase IV in rat organs during development

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

The occurrence of dipeptidyl peptidase (DPP) IV during development in Wistar rat organs was studied on day 10, 16 and 21 of gestation and on day 1, 4, 8, 13, 21, 26 and 60 after birth comparing immunohistochemistry and activity histochemistry. A polyclonal antibody, as well as monoclonal antibodies recognizing four different epitopes (A-D) of the DPP IV molecule, were employed for the immunohistochemical studies. In all investigated tissues, immunoreactivity with the polyclonal antibody appeared earlier than DPP IV activity and was already present on day 10 of gestation in the plasma membranes of embryonic and extraembryonic (decidual) cells. At these and other sites, e.g. brain capillary endothelium and tracheal or bronchial epithelium, immunoreactivity with the polyclonal antibody decreased or disappeared after birth and enzyme activity never developed. Immunoreactivity with the monoclonal antibodies appeared later than that with the polyclonal antibody, and mostly in those structures where DPP IV activity was subsequently found. The monoclonal antibody against epitope D showed a high reactivity in the epididymal duct, renal collecting ducts and in all domains of the hepatocyte plasma membrane, where neither DPP IV activity nor immunoreactivity with the other antibodies were observed. Our results also suggest that DPP IV might be present as a molecule before it becomes catalytically active and that immunoreactivity occurs at more sites than DPP IV activity. However, it cannot be excluded that the polyclonal antibody and the monoclonal antibody against the epitope D cross-react with as yet uncharacterized proteins, which express common epitopes during embryonic development, but are not present in the tissues of adult Wistar rats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bernstein, H. G., Schön, E., Ansorge, S., Rose, I. &Dorn, A. (1987) Immunolocalization of dipeptidyl peptidase IV (DPP IV) in the developing human brain.Int. J. Devl. Neuroscience 5, 237–42.

    Google Scholar 

  • Burnstone, M. S. (1962)Enzyme histochemistry and its application in the study of neoplasms. pp. 275–6. New York, London: Academic Press.

    Google Scholar 

  • Chu, S. H. W. &Walker, W. A. (1986) Developmental changes in the activities of sialyl- and fucosyltransferases in rat small intestine.Biochim. Biophys. Acta 883, 496–500.

    Google Scholar 

  • Cordell, J. L., Falini, B. &Erber, W. N. (1985) Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP) complexes.J. Histochem. Cytochem. 32, 219–29.

    Google Scholar 

  • Feizi, T. (1985) Demonstration of monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens.Nature 314, 53–7.

    Google Scholar 

  • Gass, P. &Kugler, P. (1984) Enzymdifferenzierung des visceralen Dottersackepithels der Rattle.Z. mikrosk.-anat. Forsch. 98, 721–5.

    Google Scholar 

  • Gossrau, R. (1977) Peptidasen I. Histochemische Untersuchungen mit 2-Naphthylamiden und Hexazoniumpararosanilin.Histochemistry 54, 311–30.

    Google Scholar 

  • Gossrau, R. (1979) Peptidasen II. Zur Lokalisation der Dipeptidyl peptidase IV (DPP IV). Histochemische und biochemische Untersuchungen.Histochemistry 60, 231–48.

    Google Scholar 

  • Gossrau, R. (1981) Investigation of proteinases in the digestive tract using 4-methoxy-2-naphthylamine (MNA) substrates.J. Histochem. Cytochem. 29, 464–80.

    Google Scholar 

  • Gossrau, R. (1985) Cytochemistry of membrane proteases. Histochem. J.17, 737–71.

    Google Scholar 

  • Gossrau, R., Hartel-Schenk, S., Frank, H.-G. & Reutter, W. (1990) Methodological and applied aspects of dipeptidyl peptidase (DPP) IV activity histochemistry and immunohistochemistry.Gegenbauer Morph. Jb. In press.

  • Graf, R. &Gossrau, R. (1985a) Hydrolase cytochemistry in murine rodent decidua, yolk sac and placenta with special reference to proteases.Z. mikrosk.-anat. Forsch. 99, 959–84.

    Google Scholar 

  • Graf, R. &Gossrau, R. (1985b) Cytochemische Untersuchungen von Membraneproteasen während der Leberentwicklung von Ratten.Verh. Anat. Ges. 79, 375–6.

    Google Scholar 

  • Graf, R. &Gossrau, R. (1988) Protease and γ-glutamyl transferase cytochemistry in mouse organogenesis.Biol. Zbl. 107, 189–93.

    Google Scholar 

  • Graham, R. C. &Karnovsky, N. J. (1966) The early stages of absorption of injected horse radish peroxidase in the proximal tubules of mouse kidney.J. Histochem. Cytochem. 14, 291–302.

    Google Scholar 

  • Hanski, C., Huhle, T. &Reutter, W. (1985) Involvement of plasma membrane dipeptidyl peptidase IV in fibronectin-mediated adhesion of cells on collagen.Biol. Chem. Hoppe-Seyler 366, 1169–76.

    Google Scholar 

  • Hanski, C., Huhle, T., Gossrau, R. &Reutter, W. (1988) Direct evidence for the binding of rat liver DPP IV to collagenin vitro.Exp. Cell Res. 178, 64–72.

    Google Scholar 

  • Hartels, S. (1988)Vergleichende Untersuchungen zur Dipeptidylpeptidase IV in der Ratte: Biochemische, immunologische und enzymhistochemische Charakterisierung. Dissertation, Freie Universität Berlin.

  • Hartel, S., Hanski, C., Kreisel, W., Hoffmann, C., Mauck, J. &Reutter, W. (1987) Rapid purification of dipeptidyl peptidase IV from rat liver plasma membranes.Biochim. Biophys. Acta 924, 543–7.

    Google Scholar 

  • Hartel, S., Gossrau, R., Hanski, C. &Reutter, W. (1988a) Dipeptidyl peptidase (DPP) IV in rat organs. Comparison of immunohistochemistry and activity histochemistry.Histochemistry 89, 151–61

    Google Scholar 

  • Hartel, S., Hanski, C., Neumeier, R., Cossrau, R. &Reutter, W. (1988b) Characterization of different forms of dipeptidylpeptidase IV from rat liver and hepatoma by monoclonal antibodies.Adv. Exp. Med. Biol. 240, 207–14.

    Google Scholar 

  • Heymann, E. &Mentlein, R. (1984) Beeinflusst Dipeptidyl-peptidase IV Blutdruck und Gerinnung?Klin. Wochenschr. 61, 2–10.

    Google Scholar 

  • Hong, W., Petell, J. K., Swank, D., Sanford, J., Hixson, D. J. &Doyle, D. (1989) Expression of dipeptidyl peptidase IV in rat tissues is mainly regulated at the mRNA levels.Exp. Cell Res. 182, 256–66.

    Google Scholar 

  • Hopsu-Havu, V. K. &Gienner, G. G. (1966) A new dipeptide naphthylamidase hydrolysing glycyl-prolyl-β-naphthyl-amide.Histochemie 7, 197–201.

    Google Scholar 

  • Hopsu-Havu, V. K. &Ekfors, T. O. (1969) Distrubution of a dipeptide naphthylamidase in rat tissues and its localization by using diazo coupling and labelled antibody techniques.Histochemie 17, 30–8.

    Google Scholar 

  • Imai, K. &Kato, T. (1984) Dipeptidyl aminopeptidase in neonatal rat brain regions.Experientia 40, 263–4.

    Google Scholar 

  • Kato, T., Hama, T., Nagatsu, T., Kuzuya, H. &Sakakibara, S. (1979) Changes of X-prolyl-dipeptidase activity in developing rat brain.Experientia 35, 1329–30.

    Google Scholar 

  • Kato, T., Kojima, K., Imai, K. &Nagatsu, T. (1980) Changes in the activities of collagenase-like peptidase and dipeptidylaminopeptidase IV and hydroxyproline contents in the developing rat salivary glands.Arch. Oral Biol. 25, 181–5.

    Google Scholar 

  • Köhler, G. &Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity.Nature 256, 495–7.

    Google Scholar 

  • Kreisel, W., Heussner, R., Volk, B., Büchsel, R., Reutter, W. &Gerok, W. (1982) Identification of the 110 000 Mr glycoprotein isolated from rat liver plasma membrane as dipeptidylaminopeptidase IV.FEBS Lett 147, 85–8.

    Google Scholar 

  • Lojda, Z. (1977) Studies on glycyl-proline naphthylamidase. I. Lymphocytes.Histochemistry 54, 299–309.

    Google Scholar 

  • Lojda, Z. (1979). Studies on dipeptidyl (amino) peptidase IV (glycylproline naphthylamidase). II. Blood vessels.Histochemistry 59, 153–66.

    Google Scholar 

  • Lojda, Z. (1981) Proteinase in pathology. Usefulness of histochemical methods.J. Histochem. Cytochem. 29, 481–93.

    Google Scholar 

  • Lojda, Z. (1984) Die Histochemie der Proteasen.Acta Histochem. (Jena) (Suppl)30, 9–29.

    Google Scholar 

  • Lojda, Z. (1985) The improtance of protease histochemistry in pathology.Histochem. J. 17, 1063–89.

    Google Scholar 

  • Lojda, Z. &Fric, P. (1964) Enzymes of the jejunal enterochromaffine ce,ls in man.Histochemie 3, 455–61.

    Google Scholar 

  • Lojda, Z., Gossrau, R. &Schiebler, T. H. (1979)Enzyme histochemistry.A laboratory manual. Berlin Heidelberg New York: Springer.

    Google Scholar 

  • Lojda, Z., Smidowa, J., Barth, A. &Ueberberg, H. (1988) Are Z-Arg-Gly-Phe-Phe-Leu-MNA and Z-Arg-Gly-Phe-Phe-Pro-MNA suitable substrates for the demonstration of cathepsin D activity?Histochemistry 88, 505–12.

    Google Scholar 

  • MacNair, R. C. D. &Kenny, A. J. (1979) Proteins of the kidney microvillar membrane. The amphipatic form of dipeptidyl peptidase IV.Biochem. J. 179, 379–95.

    Google Scholar 

  • McDonald, J. K. &Barrett, A. J. (1986)Mammalian proteases. A glossary and bibliography, Vol. 2, exopeptidases. New York: London: Academic Press.

    Google Scholar 

  • McGadey, J. (1970) A tetrazolium method for nonspecific alkaline phosphatase.Histochemie 23, 180–4.

    Google Scholar 

  • Neiss, W. F. &Klehn, K. L. (1981) The postantal development of the rat kidney, with special reference to the characterization of the proximal tubule.Histochemistry 73, 251–68.

    Google Scholar 

  • Piazza, G. A., Callanan, H. M., Mowery, J. & Hixson, D. C. (1988) Evidence for a role of dipeptidyl peptidase IV in fibronectin-mediated interactions of hepatocytes with extracellular matrix.Biochem. J. in press.

  • Roth, J., Taatjes, D. J., Bitter-Suermann, D. &Finne, J. (1987) Polysialic acid units are spatially expressed in developing postnatal rat kidney.Proc. Natl. Acad. Sci. USA 84, 1969–73.

    Google Scholar 

  • Sahara, N., Fukasawa, K. M., Fukasawa, K., Araki, N. &Suzuki, K. (1981) Immunohistochemical localization of dipeptidyl aminopeptidase (DAP) IV in the rat submandibular gland during postnatal development.Histochemistry 72, 229–36.

    Google Scholar 

  • Stutte, H. J. (1967) Hexazotiertes Triamino-tritolylmethan-chlorid (Neufuchsin) als Kupplungssalz in der Fermenthistochemie.Histochemie 8, 327–31.

    Google Scholar 

  • Tauc, M., Chatelet, F., Verroust, P., Vandewall, A., Poujeol, P. &Ronco, P. (1988) Characterization of monoclonal antibodies specific for rabbit renal brushborder hydrolases: Application of immunohistochemical localization.J. Histochem. Cytochem. 36, 523–32.

    Google Scholar 

  • Tauc, M., Merot, J., Bidet, M., Koehlin, N., Gastineau, M., Othmani, L. &Poujeol, P. (1989) Antigenic expression of aminopeptidase M, dipeptidyl peptidase IV and endopeptidase by primary cultures from rabbit kidney proximal tubule.Histochemistry 91, 17–30.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimalee 22, D-1000, Berlin 33 (Dahlem), Germany

    S. Hartel-Schenk & W. Reutter

  2. Institut für Anatomie, Freie Universität Berlin, Königin-Luise-Strasse 15, D-1000, Berlin 33 (Dahlem), Germany

    R. Gossrau

Authors
  1. S. Hartel-Schenk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Gossrau
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. Reutter
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hartel-Schenk, S., Gossrau, R. & Reutter, W. Comparative immunohistochemistry and histochemistry of dipeptidyl peptidase IV in rat organs during development. Histochem J 22, 567–578 (1990). https://doi.org/10.1007/BF01005980

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01005980

Keywords

Search

Navigation