Summary
A simple modification to the silver staining technique for the demonstration of nucleolar organizer region associated proteins is described. Polyethylene glycol 20 000 is used instead of gelatin as the colloidal developer. This modified technique remains a one stage procedure that is quick and easy to perform. It results in reduced precipitate and less non-specific staining with specimens in which it had been previously difficult to demonstrate these intranuclear silver staining structures.
Similar content being viewed by others
References
Boldy, D. A. R., Crocker, J. &Ayres, J. G. (1989) Application of the AgNOR method to cell imprints of lymphoid tissue.J. Pathol. 157, 75–9.
Chiu, K. Y., Loke, S. L. &Wong, K. K. (1989) Improved silver technique for showing nucleolar organizer regions in paraffin wax sections.J. Clin. Pathol. 42, 992–4.
Cromie, C. J., Benbow, E. W., Stoddart, R. W. &McMahon, R. F. T. (1988) Preincubation with a glycine solution aids the demonstration of nucleolar organizer region-associated protein.Histochem. J. 20, 722–4.
Goodpasture, C. &Bloom, S. E. (1975) Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining.Chromosoma 53, 37–50.
Howell, W. M., Denton, T. E. &Diamond, J. R. (1975) Differential staining of the satellite regions of human acrocentric chromosomes.Experientia 31, 260–2.
Howell, W. M. &Black, D. A. (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method.Experientia 36, 1014–15.
McMeekin, W., Kennedy, A. &McNicol, A. M. (1989) Combined immunocytochemistry and nucleolar organizer region staining: some technical aspects.Med. Lab. Sci. 46, 11–15.
Murray, P. G., Boldy, D. A. R., Crocker, J. &Ayres, J. G. (1989) Sequential demonstration of antigens and AgNORs in frozen and paraffin sections.J. Pathol. 159, 169–72.
Ploton, D., Menager, M., Jeannesson, P., Himber, G., Pigeon, F. &Adnet, J. J. (1986) Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level.Histochem. J. 18, 5–14.
Schwarzacher, H. G. &Wachtler, F. (1983) Nucleolus organizer regions and nucleoli.Hum. Genet. 63, 89–99.
Smith, R. &Crocker, J. (1988) Evaluation of nucleolar organizer region-associated proteins in breast malignancy.Histopathol. 12, 113–25.
Thiebaut, F., Rigaut, J. P. &Reith, A. (1984) Improvement in the specificity of the silver staining technique for AgNOR-associated acidic proteins in paraffin sections.Stain Technol. 59, 181–5.
Underwood, J. C. E. &Giri, D. D. (1988) Nucleolar organizer regions as diagnostic discriminants for malignancy.J. Pathol. 155, 95–6.
Walker, R. A. (1988) The histopathological evaluation of nucleolar organizer region proteins.Histopathol. 12, 221–3.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rowlands, D.C., Crocker, J. & Ayres, J.G. Silver staining of nucleolar organizer region associated proteins using polyethylene glycol as the protective colloidal developer. Histochem J 22, 555–559 (1990). https://doi.org/10.1007/BF01005978
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01005978