Synopsis
A method is described for the histochemical localization of glucose dehydrogenase activity in sheep liver. Fresh-frozen sections were fixed in acetone at −20°C and incubated in a medium at pH 8.0 containing glucose, NAD or NADP, Nitro BT and EDTA.p-Hydroxymercuribenzoate was added to suppress the ‘nothing dehydrogenase’ effect. Activity was mainly confined to hepatocyte cytoplasm and, with NAD, was strongest in periportal areas. Activity was less with NADP and was strongest in centrilobular areas. The specificity of the enzyme for different substrates and inhibitors was compatible with the known properties of glucose dehydrogenase.
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Manns, E. The histochemical localization of glucose dehydrogenase activity in sheep liver. Histochem J 4, 25–33 (1972). https://doi.org/10.1007/BF01005266
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DOI: https://doi.org/10.1007/BF01005266