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Differentiation of Purkinje fibres and ordinary ventricular and atrial myocytes in the bovine heart: an immuno-and enzyme histochemical study

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Summary

The differentiation of Purkinje fibres and ordinary ventricular and atrial myocytes in bovine hearts was studied with specific antibodies against M-line proteins (MM-creatine kinase and myomesin) and with enzyme histochemistry (succinate dehydrogenase and mitochondrial glycerol-3-phosphate dehydrogenase). MM-creatine kinase was detected at an earlier stage in Purkinje fibres and atrial myocytes than in ordinary ventricular myocytes. The findings are in agreement with previous ultrastructural observations that an earlier appearance of a dense M-band occurs in Purkinje fibres than in ordinary ventricular myocytes. Myomesin was detected in all three cell types even at early foetal stages, in accordance with suggestions that it is an integral component of the myofibrillar structure. The activity of succinate dehydrogenase gradually increased in both ordinary ventricular and atrial myocytes, while the activity of mitochondrial glycerol-3-phosphate dehydrogenase was high at different stages of early foetal development in the two tissues, finally becoming low in the adult stage. The activity of succinate dehydrogenase and mitochondrial glycerol-3-phosphate dehydrogenase seemed to remain unchanged in the Purkinje fibres from early to late foetal stages. The present study shows that the Purkinje fibres are already different from ordinary ventricular myocytes at early foetal stages and that the two cell types differentiate in different ways. It is concluded that there are also developmental differences between ordinary ventricular and atrial myocytes.

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References

  • Allen, E. R. &Pepe, F. A. (1965) Ultrastructure of developing muscle cells in the chick embryo.Am. J. Anat. 116, 115–48.

    Google Scholar 

  • Anversa, P., Olivetti, G., Bracchi, P.-G. &Loud, A. V. (1981) Postnatal development of the M-band in rat cardiac myofibrils.Circ. Res. 48, 561–8.

    Google Scholar 

  • Cooper, W. G. (1955) Succinic dehydrogenase activity in the pre-natal and post-natal rat heart.Anat. Rec. 123, 103–24.

    Google Scholar 

  • Davies, M. J. (1971)Pathology of Conducting Tissue of the Heart. pp. 20–28. London: Butterworth.

    Google Scholar 

  • Dehaan, R. L. (1961) Differentiation of the atrioventricular conducting system of the heart.Circulation 24, 458–70.

    Google Scholar 

  • Dubowitz, V. &Brooke, M. H. (1973) Muscle biopsy: A modern approach. pp. 20–33. London: Saunders.

    Google Scholar 

  • Eppenberger, H. M., Perriard, J.-C., Rosenberg, U. B. &Strehler, E. E. (1981) The Mr 165,000 M-protein myomesin: A specific protein of cross-striated muscle cells.J. Cell Biol. 89, 185–93.

    Google Scholar 

  • Eppenberger, H. M., Wallimann, T., Kuhn, H. J. &Turner, D. C. (1975) Localization of creatine kinase isoenzymes in muscle cells: Physiological significance. InIsozymes II (edited byMarkert, C.), pp. 409–24. New York: Academic Press.

    Google Scholar 

  • Eriksson, A., Thornell, L.-E. &Stigbrand, T. (1979) Skeletin immunoreactivity in heart Purkinje fibers of several species.J. Histochem. Cytochem. 27, 1604–9.

    Google Scholar 

  • Etlinger, J. D. &Fischman, D. A. (1973) M-and Z-band components and the assembly of myofibrils.Cold Spring Harb. Symp. quant. Biol. 37, 511–22.

    Google Scholar 

  • Fischman, D. A. (1970) The synthesis and assembly of myofibrils in embryonic muscle.Curr. Topics Devl Biol. 5, 235–80.

    Google Scholar 

  • Forsgren, S., Carlsson, E., Strehler, E. & Thornell, L.-E. (1982) Ultrastructural identification of human fetal Purkinje fibres—A comparative immunocytochemical and electron icroscopic study of composition and structure of myofibrillar M-regions.J. molec. Cell. Cardiol. (in press).

  • Forsgren, S. &Thornell, L.-E. (1981) The development of Purkinje fibres and ordinary myocytes in the bovine fetal heart. An ultrastructural study.Anat. Embryol. 162, 127–36.

    Google Scholar 

  • Forsgren, S., Thornell, L.-E. &Eriksson, A. (1980) The development of the Purkinje fibre system in the bovine fetal heart.Anat. Embryol. 159, 125–35.

    Google Scholar 

  • Gossrau, R. (1968) Über das Reizleitungssystem der Vögel. Histochemische und elektronenmikroskopische Untersuchungen.Histochemie 13, 111–59.

    Google Scholar 

  • Greenfield, P. C. &Boell, E. J. (1968) Succinic dehydrogenase and cytochrome oxidase of mitochondria of chick liver, heart and skeletal muscle during embryonic development.J. exp. Zool. 168, 491–500.

    Google Scholar 

  • Harary, I. (1979) Biochemistry of cardiac development:in vivo andin vitro studies. InHandbook of Physiology. The Cardiovascular System (edited byBerne, R. M., Sperelakis, N. andGeiger, S. R.), Vol. 1, pp. 43–60. Bethesda, Maryland: American Physiological Society.

    Google Scholar 

  • Hirakow, R., Gotoh, T. &Watanabe, T. (1980) Quantitative studies on the ultrastructural differentiation and growth of mammalian cardiac muscle cells. I. The atria and ventricles of the rat.Acta Anat. 108, 144–52.

    Google Scholar 

  • Kilarski, W. &Jakubowska, M. (1979) An electron microscope study of myofibril formation in embryonic rabbit skeletal muscle.Z. mikrosk.-anat. Forsch. 93, 1159–81.

    Google Scholar 

  • Mackler, B., Grace, R. &Duncan, H. M. (1971) Studies of mitochondrial development during embryogenesis in the rat.Archs Biochem. Biophys. 144, 603–10.

    Google Scholar 

  • Markwald, R. R. (1973) Distribution and relationship of precursor Z-material to organizing myofibrillar bundles in embryonic rat and hamster ventricular myocytes.J. molec. Cell. Cardiol. 5, 341–50.

    Google Scholar 

  • Meijer, A. E. F. H. &Devries, G. P. (1978) Enzyme histochemical studies on the Purkinje fibres of the atrioventricular system of the bovine and porcine hearts.Histochem. J. 10, 399–408.

    Google Scholar 

  • Nandy, K. &Bourne, G. H. (1963) A study of the morphology of the conduction tissue in mammalian hearts.Acta Anat. 53, 217–26.

    Google Scholar 

  • Oliphant, L. W. &Loewen, R. D. (1976) Filament systems in Purkinje cells of the sheep heart: possible alterations of myofibrillogenesis.J. molec. Cell. Cardiol. 8, 679–88.

    Google Scholar 

  • Page, E., Power, B., Fozzard, H. A. &Meddoff, D. A. (1969) Sarcolemmal evaginations with knob-like or stalked projections in Purkinje fibres of the sheep's heart.J. Ultrastruct. Res. 28, 288–300.

    Google Scholar 

  • Peter, J. B., Barnard, R. J., Edgerton, V. R., Gillespie, C. A. &Stempel, K. E. (1972) Metabolic profiles of three fiber types of skeletal muscle in guinea pigs and rabbits.Biochemistry 11, 2627–33.

    Google Scholar 

  • Robb, J. S. (1965)Comparative Basic Cardiology. New York: Grune & Stratton.

    Google Scholar 

  • Schifbler, T. H., Stark, M. &Caesar, R. (1956) Die Stoffwechsel-situation des Reizleitungssystems.Klin. Wschr. 34, 181–3.

    Google Scholar 

  • Schiebler, T. H. (1961) Histochemische Untersuchungen am Reizleitungssystem tierischer Herzen.Naturwissenschaften 14, 502–3.

    Google Scholar 

  • Skala, J., Drahota, Z. &Hahn, P. (1970) Succinate dehydrogenase activity of rat heart muscle mitochondria during development.Physiol. bohemoslov. 19, 15–7.

    Google Scholar 

  • Smith, H. E. &Page, E. (1977) Ultrastructural changes in rabbit heart mitochondria during the perinatal period.Dev. Biol. 57, 109–17.

    Google Scholar 

  • Snijder, J. &Meijer, A. E. F. H. (1970) Enzyme histochemical studies on the Purkinje fibres of canine, bovine and porcine hearts.Histochem. J. 2, 395–409.

    Google Scholar 

  • Sordahl, L. A., Crow, C. A., Kraft, G. H. &Schwartz, A. (1972) Some ultrastructural and biochemical aspects of heart mitochondria associated with development: fetal and cardiomyopathic tissue.J. molec. Cell. Cardiol. 4, 1–10.

    Google Scholar 

  • Strehler, E. E., Pelloni, G., Heizman, C. W. &Eppenberger, H. M. (1979) M-protein in chicken cardiac muscle.Expl Cell Res. 124, 39–45.

    Google Scholar 

  • Strehler, E. E., Pelloni, G., Heizman, C. W. &Eppenberger, H. M. (1980) Biochemical and ultrastructural aspects of Mr 165,000 M-protein in cross-striated chicken muscle.J. Cell Biol. 86, 775–83.

    Google Scholar 

  • Tanimura, T. &Shepard, T. H. (1970) Glucose metabolism by rat embryosin vitro.Proc. Soc. exp. Biol. Med. 135, 51–4.

    Google Scholar 

  • Thornell, L.-E. (1974) Distinction of glycogen and ribosome particles in cow Purkinje fibres by enzymatic digestion en bloc and in sections.J. Ultrastruct. Res. 49, 157–66.

    Google Scholar 

  • Toth, A. &Schiebler, T. H. (1967) Über die Entwicklung der Arbeits- und Erregungsleitungsmuskulatur des Herzens von Ratte und Meerschweinchen. Histologische, histochemische und elektrophysiologische Untersuchungen.Z. Zellforsch. 76, 543–67.

    Google Scholar 

  • Truex, R. C. &Smythe, M. Q. (1965) Comparative morphology of the cardiac conduction tissue in animals.Ann. N.Y. Acad. Sci. 127, 19–33.

    Google Scholar 

  • Turner, D. C., Wallimann, T. &Eppenberger, H. M. (1973) A protein that binds specifically to the M-line of skeletal muscle is identified as the muscle form of creatine kinase.Proc. natn. Acad. Sci., U.S.A. 70, 702–5.

    Google Scholar 

  • Virách, S. &Challice, C. E. (1977) The development of the conduction system in the mouse embryo heart.Dev. Biol. 56, 397–411.

    Google Scholar 

  • Wallimann, T., Pelloni, G., Turner, D. C. &Eppenberger, H. M. (1978) Monovalent antibodies against MM-creatine kinase remove the M-line from myofibrils.Proc. natn. Acad. Sci., U.S.A. 75, 4296–300.

    Google Scholar 

  • Wallimann, T., Turner, D. C. &Eppenberger, H. M. (1977) Localization of creatine kinase isoenzymes in myofibrils. I. Chicken skeletal muscle.J. Cell Biol. 75, 297–317.

    Google Scholar 

  • Wendler, D. (1967) Die morphologische und chemische Organisation des fetalen und reifen Myokards.Z. inn. Med. 22, 737–46.

    Google Scholar 

  • Weidmann, S. (1971) The Purkyne fibres of ungulate hearts. InCentenary Symposium (edited byKruta, V.), p. 227. Brno: Universita Jana Evangelisty Purkyne.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Anatomy, University of Umeå, S-901 87, Umeå, Sweden

    Sture Forsgren & Lars-Eric Thornell

  2. Institute of Cell Biology, Swiss Federal Institute of Technology, CH-8093, Zürich, Switzerland

    Emanuel Strehler

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  1. Sture Forsgren
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  2. Emanuel Strehler
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  3. Lars-Eric Thornell
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Forsgren, S., Strehler, E. & Thornell, LE. Differentiation of Purkinje fibres and ordinary ventricular and atrial myocytes in the bovine heart: an immuno-and enzyme histochemical study. Histochem J 14, 929–942 (1982). https://doi.org/10.1007/BF01005234

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  • DOI: https://doi.org/10.1007/BF01005234

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