Synopsis
A layer of collagenous connective tissue develops around the ejaculatory duct of the male locust,Locusta migratoria, during the fourth and fifth instars and the first ten days of the adult stage. The mucosubstances associated with this tissue have been characterized by using a series of histochemical reactions, including Alcian Blue staining at different pH levels and salt concentrations the periodic acid-Schiff test and recent modifications of it, the high iron diamine test and enzymatic digestions. The results indicate that sulphated glycosaminoglycans accumulate during the developmental period, so that in the mature adult, the connective tissue probably contains chondroitin and dermatan sulphates, and possibly some keratan sulphate. Neutral glycoproteins also occur in the tissue.
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References
Ashhurst, D. E. (1959). The connective tissue sheath of the locust nervous system: a histochemical study.Q. Jl microsc. Sci. 100, 401–12.
Ashhurst, D. E. (1961). A histochemical study of the connective-tissue sheath of the nervous system ofPeriplaneta americand.Q. Jl microsc. Sci. 102, 455–61.
Ashhurst, D. E. (1968). The connective tissues, of insects.A. Rev. Ent. 13, 45–74.
Ashhurst, D. E. &Costin, N. M. (1971). Insect mucosubstances. II. The mucosubstances of the central nervous system.Histochem. J. 3, 297–310.
Ashhurst, D. E. &Patel, N. G. (1963). Hyaluronic acid in cockroach ganglia.Ann. ent. Soc. Am. 56, 182–4.
Ashhurst D. E. &Richards, A. G. (1964). The histochemistry of the connective tissue associated with the central nervous system of the, pupa of the wax-moth,Galleria mellonella L.J. Morph. 114, 237–46.
Baccetti, B. (1955). Ricerche sulla fine struttura del, perilemma nel sistema nervoso degli insetti.Redia 40, 197–212.
Baccetti, B. (1956). Lo stroma di sostegna di organi degli insetti esaminato a luce polarizzata.Redia 41, 259–76.
Estes, Z. E. &Faust, R. M. (1964). Studies on the mucopolysaccharides of the greater wax-moth,Galleria mellonella (Linnaeus)Comp. Biochem. Physiol. 13, 443–52.
Gregory, G. E. (1965). The formation and fate of the spermatophore of the African migratory locust,Locusta migratoria migratorioides Reiche & Fairmaire.Trans. R.ent. Soc. Lond. 117, 33–66.
Katzman, R. L. &Jeanloz, R. W. (1970) The carbohydrate chemistry of invertebrate connective tissue. InChemistry and Molecular Biology of the Intercellular Matrix, ed. E. A. Balazs, pp. 217–27. London & New York: Academic Press.
Kefalides, N. A. (1970). Comparative biochemistry of mammalian basement membrane. InChemistry and Molecular Biology of the Intercellular Matrix, ed. E. A. Balazs. pp. 535–73. London & New York: Academic Press.
Leblond, C. P., Clegg, R. E. &Eidinger, D. (1957). Presence of carbohydrates with free 1,2-glycol groups in sites stained by the periodic acid-Schiff technique.J. Histochem. Cytochem. 5, 445–58.
Lillie, R. D. (1965).Histopathologic Technic and Practical Histochemistry, 3rd Ed., p. 715. New York: McGraw-Hill.
Marshall, A. T. (1966). Spittle-production and tube-building by cercopid larvae (Homoptera). IV. Mucopolysaccharide associated with spittle production.J. Insect Physiol. 12, 635–44.
Martoja, R. (1970). Sur la sous-cuticle et la basale du canal éjaculateur d'un insecteLocusta migratoria (Orthoptère).J. Microscopie 9, 643–52.
Martoja, R. &Bassot J.-M. (1965). Existence d'un tissu, conjonctif de type cartilagineux chez certains insectes Orthoptères.C.r. Acad. Sci. Paris 261, 2954–7.
Mathews, M. B. (1967). Macromolecular evolution of connective tissue.Biol. Rev. 42, 499–551.
Mathews, M. B. (1970). The interactions of proteoglycans and collagen-model systems. InChemistry and Molecular Biology of the Intercellular Matrix, ed. E. A. Balazs, pp. 1155–69. London & New York: Academic Press.
Mowry, R. W. &Scott, J. B. (1967). Observations on the basophilia of amyloids.Histochemie 10, 8–32.
Odhiambo, T. R. (1970). The architecture of the accessory reproductive glands of the male desert locust. III. Components of the muscular wall.Tissue & Cell 2, 233–48.
Pearse, A. G. E. (1968).Histochemistry: Theoretical, and Applied, 3rd Ed.,, p. 759. London: Churchill.
Quintarelli, G., Scott, J. E. &Dellovo, M. C. (1964a). The chemical and histochemical properties of Alcian Blue. II. Dye binding of tissue polyanions.Histochemie 4, 86–98.
Quintarelli, G., Scott, J. E. &Dellovo, M. C. (1964b). The chemical and histochemical properties of Alcian Blue. III. Chemical blocking and unblocking.Histochemie 4, 99–112.
Scott, J. E. (1967). On the mechanism of the methyl green-pyronin stain for, nucleic acids.Histochemie 9, 30–47.
Scott, J. E. (1970). Histochemistry of Alcian Blue. I.Metachromasia of Alcian Blue, Astrablau and other cationic phthalocyanin dyes.Histochemie 21, 277–85.
Scott, J. E. &Dorling, J. (1965). Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian Blue in salt solutions.Histochemie 5, 221–33.
Scott, J. E. &Dorling, J. (1969). Periodate oxidation of acid polysaccharides. III. A PAS method for chondroitin sulphates and other glycosamino-glycuronans.Histochemie 19, 295–301.
Scott, J. E., Dorling, J. &Stockwell, R. A. (1968). Reversal of protein blocking of basophilia in salt solutions: implications in the localisation of polyanions using Alcian Blue.J. Histochem. Cytochem. 16, 383–86.
Scott, J. E. &Harbinson, R. J. (1969). Periodate oxidation of acid polysaccharides. II. Rates of oxidation of uronic acids in, polyuronides and acid mucopolysaccharides.Histochemie 19, 155–61.
Scott, J. E. &Quintarelli, G. (1965). Staining properties of Alcian Blue. I. The mechanism of staining.J. Histochem. Cytochem. 13, 29–30.
Scott, J. E., Quintarelli, G. &Dellovo, M. C. (1964). The chemical and histochemical properties of Alcian Blue. I. The mechanism of Alcian Blue staining.Histochemie 4, 73–85.
Spicer, S. S. (1965). Diamine methods for differentiating mucosubstances histochemically.J. Histochem. Cytochem. 13, 211–34.
Spicer, S. S., Horn, R. G. &Leppi, T. J. (1967). Histochemistry of connective tissue mucopolysaccharides. InThe Connective Tissue, eds. B. M. Wagner & D. E. Smith, pp. 251–303. Baltimore: Williams & Wilkins.
Spiro, R. G. (1970a). The carbohydrate of collagens. InChemistry and Molecular Biology of the Intercellular Matrix, ed. E. A. Balazs, pp. 195–215. London & New York: Academic Press.
Spiro, R. G. (1970b). Biochemistry of basement membranes. InChemistry and Molecular Biology of the Intercellular Matrix, ed. E. A. Balazs, pp. 511–34. London & New York: Academic Press.
Stockwell, R. A. &Scott, J. E. (1965). Observations on the acid glycosaminoglycan (mucopolysaccharide) content of the matrix of aging cartilage.Ann. rheum. Dis. 24, 341–9.
Stoward, P. J. (1967) The histochemical properties of some periodate reactive mucosubstances of the pregnant Syrian hamster before and after methylation with methanolic thionyl chloride.Jl R. microsc. Soc. 87, 77–103.
Zugibe, F. T. (1970). Positive periodic acid-Schiff staining of acid mucopolysaccharides.Histochem J. 2, 191–7.
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Ashhurst, D.E., Costin, N.M. Insect mucosubstances. I. The mucosubstances of developing connective tissue in the locust,Locusta migratoria . Histochem J 3, 279–295 (1971). https://doi.org/10.1007/BF01005227
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DOI: https://doi.org/10.1007/BF01005227