The Histochemical Journal

, Volume 5, Issue 4, pp 343–349 | Cite as

The simultaneous demonstration of adrenergic fibres and enteric ganglion cells

  • M. Costa
  • J. B. Furness
Papers

Synopsis

A method for the demonstration of adrenergic nerves and enteric neurons at the same time has been developed by combining the fluorescence histochemical technique for catecholamines and the histochemical technique for NADH:Nitro BT oxidoreductase.

The method consists of a short incubation of the laminae from the wall of the intestine in an isotonic medium containing the substrate (NADH) and a tetrazolium salt (Nitro BT). After washing, the laminae are air dried, exposed to formaldehyde vapour and mounted.

The adrenergic nerves in the myenteric plexus appear brightly fluorescent on excitation with u.v. light, whereas the neurons are heavily stained by deposits of formazan. Not all the neurons of the plexus are stained, but their morphology is well preserved. Differences in staining of the neurons reflect differences in penetration of the tetrazolium salt in the tissue and into the cells. The adrenergic axons do not establish exclusive connexions with individual neurons and some isolated neurons are not associated with any adrenergic fibres.

Keywords

Formaldehyde NADH Catecholamine Ganglion Cell Formazan 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barrnett, R. J. (1959). The demonstration with the electron microscope of the end products of histochemical reactions in relation to the fine structure of cells.Exp. Cell Res. suppl. 7, 65–89.Google Scholar
  2. Björklund, A., Falck, B. &Owman, Ch. (1972). Fluorescence microscopic and microspectrofluorometric techniques for the cellular localization and characterization of biogenic amines. InThe Thyroid and Biogenic Amines (eds. J. E. Rall and I. J. Kopin), pp. 318–68. Amsterdam: North Holland.Google Scholar
  3. Costa, M. &Gabella, G. (1971). Adrenergic innervation of the alimentary canal.Z. Zellforsch. mikrosk. Anat. 122, 357–77.Google Scholar
  4. Furness, J. B. (1970). The origin and distribution of adrenergic fibres in the guinea-pig colon.Histochemie 27, 295–306.Google Scholar
  5. Furness, J. B. & Costa, M. (1973). The adrenergic innervation of the gastrointestinal tract.Ergebn. d. Physiol. in press.Google Scholar
  6. Gabella, G. (1969). Detection of nerve cells by a histochemical method.Experientia (Basel) 25, 218–19.Google Scholar
  7. Gabella, G. &Costa, M. (1967). Le fibre adrenergiche nel canale alimentare.Giorn. Accad. Med. Torino 130, 1–12.Google Scholar
  8. Jacobowitz, D. (1965). Histochemical studies of the autonomic innervation of the gut.J. Pharmac. exp. Ther. 149, 358–64.Google Scholar
  9. Lehninger, A. L. (1951). Phosphorylation coupled to oxidation of dihydrodiphosphopyridine nucleotide.J. biol. Chem. 190, 345–59.Google Scholar
  10. Norberg, K. A. (1964). Adrenergic innervation of the intestinal wall studied by fluorescence microscopy.Int. J. Neuropharmacol. 3, 379–82.Google Scholar
  11. Novikoff, A. B. (1960). Biochemical and staining reactions of cytoplasmic constituents. InDeveloping Cell Systems and their Control (ed. D. Rudnick), pp. 167–203. New York: The Ronald Press.Google Scholar
  12. Ohkawa, H. &Prosser, C. L. (1972a). Electrical activity in myenteric and submucous plexuses of cat intestine.Am. J. Physiol. 222, 1412–19.Google Scholar
  13. Ohkawa, H. &Prosser, C. L. (1972b). Functions of neurons in enteric plexuses of cat intestine.Am. J. Physiol. 222, 1420–6.Google Scholar
  14. Pearse, A. G. E. (1972).Histochemistry, Theoretical and Applied, Vol. 2. Edinburgh and London: Churchill, Livingstone.Google Scholar
  15. Rosenbaum, R. M. (1964). The intracellular localization of enzymes. InHandbuch der Histochemie (eds. W. Graumann and K. Neumann), Vol. 7, part 4, pp. 1–108. Stuttgart: Gustav Fisher Verlag.Google Scholar
  16. Wainio, W. W. (1970).The Mammalian Mitochondrial Respiratory Chain. New York, London: Academic Press.Google Scholar

Copyright information

© Chapman and Hall Ltd 1973

Authors and Affiliations

  • M. Costa
    • 1
  • J. B. Furness
    • 1
  1. 1.Department of ZoologyUniversity of MelbourneParkvilleAustralia

Personalised recommendations